Problems Associated with the Identification of Proteins in Homologous Families: The Wool Keratin Family as a Case Study

The keratin proteins from wool can be divided into two classes: the intermediate filament proteins (IFPs) and the matrix proteins. Using peptide mass spectral fingerprinting it was possible to match spots to the known theoretical sequences of some IFPs in web-based databases, as enzyme digestion gen...

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Veröffentlicht in:	Analytical biochemistry 2002-01, Vol.300 (2), p.221-229
Hauptverfasser:	Plowman, Jeffrey E., Bryson, Warren G., Flanagan, Leanne M., Jordan, T.William
Format:	Artikel
Sprache:	eng
Schlagworte:	2-D electrophoresis Amino Acid Sequence Animals Electrophoresis, Gel, Two-Dimensional keratins Keratins - chemistry Keratins - classification Molecular Sequence Data Molecular Weight Multigene Family Peptide Fragments - chemistry Peptide Mapping peptide mass fingerprinting proteomics Sequence Homology, Amino Acid Sheep Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Wool - chemistry wool proteins
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