Separation of γ-globulins from porcine plasma by reversed micellar extraction
Factors affecting the separation of γ-globulins from porcine plasma using reversed micelles were screened based on a fractional factorial design. The optimal processing conditions for obtaining the maximum yield of γ-globulins under various constraints of product purity were determined using respons...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2001, Vol.92 (3), p.214-220 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Yang, Tang Huang Yet, Mei Gang Hsu, Cheng Kuang Chiang, Been Huang |
| description | Factors affecting the separation of γ-globulins from porcine plasma using reversed micelles were screened based on a fractional factorial design. The optimal processing conditions for obtaining the maximum yield of γ-globulins under various constraints of product purity were determined using response surface methodology (RSM) and nonlinear programming. Results showed that the pH and sodium chloride concentration of the aqueous phase, and the concentration of surfactant (bis-(2-ethylhexyl) sulfosuccinate sodium salt, AOT) of the organic phase were the most important factors affecting the extraction performance. An eighty-five percent product purity and ninety-seven percent yield were obtained under the extraction conditions of 400 mM NaCl, 350 mM AOT, and pH 7.0. The extract exhibited immunological reactivity against anti-pig IgG. |
| doi_str_mv | 10.1016/S1389-1723(01)80252-8 |
| format | Article |
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The optimal processing conditions for obtaining the maximum yield of γ-globulins under various constraints of product purity were determined using response surface methodology (RSM) and nonlinear programming. Results showed that the pH and sodium chloride concentration of the aqueous phase, and the concentration of surfactant (bis-(2-ethylhexyl) sulfosuccinate sodium salt, AOT) of the organic phase were the most important factors affecting the extraction performance. An eighty-five percent product purity and ninety-seven percent yield were obtained under the extraction conditions of 400 mM NaCl, 350 mM AOT, and pH 7.0. 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The optimal processing conditions for obtaining the maximum yield of γ-globulins under various constraints of product purity were determined using response surface methodology (RSM) and nonlinear programming. Results showed that the pH and sodium chloride concentration of the aqueous phase, and the concentration of surfactant (bis-(2-ethylhexyl) sulfosuccinate sodium salt, AOT) of the organic phase were the most important factors affecting the extraction performance. An eighty-five percent product purity and ninety-seven percent yield were obtained under the extraction conditions of 400 mM NaCl, 350 mM AOT, and pH 7.0. The extract exhibited immunological reactivity against anti-pig IgG.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>BLOOD PLASMA</subject><subject>Concentration (process)</subject><subject>Constraint theory</subject><subject>EXTRACTION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. Pharmaceutical industry</subject><subject>IMMUNOGLOBULINS</subject><subject>Industrial applications and implications. 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Psychology</topic><topic>Health. Pharmaceutical industry</topic><topic>IMMUNOGLOBULINS</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Micelles</topic><topic>Nonlinear programming</topic><topic>Other active biomolecules</topic><topic>porcine plasma</topic><topic>Production of active biomolecules</topic><topic>reversed micelles</topic><topic>SEPARATING</topic><topic>separation</topic><topic>Surface active agents</topic><topic>SWINE</topic><topic>γ-globulins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Tang Huang</creatorcontrib><creatorcontrib>Yet, Mei Gang</creatorcontrib><creatorcontrib>Hsu, Cheng Kuang</creatorcontrib><creatorcontrib>Chiang, Been Huang</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Tang Huang</au><au>Yet, Mei Gang</au><au>Hsu, Cheng Kuang</au><au>Chiang, Been Huang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of γ-globulins from porcine plasma by reversed micellar extraction</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2001</date><risdate>2001</risdate><volume>92</volume><issue>3</issue><spage>214</spage><epage>220</epage><pages>214-220</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Factors affecting the separation of γ-globulins from porcine plasma using reversed micelles were screened based on a fractional factorial design. 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| ispartof | Journal of bioscience and bioengineering, 2001, Vol.92 (3), p.214-220 |
| issn | 1389-1723 1347-4421 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences Biotechnology BLOOD PLASMA Concentration (process) Constraint theory EXTRACTION Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry IMMUNOGLOBULINS Industrial applications and implications. Economical aspects Micelles Nonlinear programming Other active biomolecules porcine plasma Production of active biomolecules reversed micelles SEPARATING separation Surface active agents SWINE γ-globulins |
| title | Separation of γ-globulins from porcine plasma by reversed micellar extraction |
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