Affinity purification of PSD‐95‐containing postsynaptic complexes

A widely used method for the preparation of postsynaptic density (PSD) fractions consists of treatment of synaptosomal membranes with Triton X‐100 and further purification by density gradient centrifugation. In the present study, the purity of this preparation was assessed by electron microscopic an...

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Veröffentlicht in:	Journal of neurochemistry 2003-12, Vol.87 (5), p.1255-1261
Hauptverfasser:	Vinade, Lucia, Chang, Michael, Schlief, Michelle L., Petersen, Jennifer D., Reese, Thomas S., Tao‐Cheng, Jung‐Hwa, Dosemeci, Ayse
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing affinity purification anchoring proteins Animals Antibodies - chemistry Biological and medical sciences Brain Chemistry Cell membranes. Ionic channels. Membrane pores Cell structures and functions Disks Large Homolog 4 Protein Fundamental and applied biological sciences. Psychology glutamate receptors Immunoblotting Immunomagnetic Separation - methods Immunosorbent Techniques Intracellular Signaling Peptides and Proteins Macromolecular Substances Membrane Proteins Microscopy, Immunoelectron Molecular and cellular biology Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - immunology Nerve Tissue Proteins - isolation & purification Nerve Tissue Proteins - metabolism Octoxynol - chemistry postsynaptic density PSD‐95 Rats Rats, Sprague-Dawley Receptors, AMPA - chemistry Receptors, AMPA - metabolism Subcellular Fractions - chemistry
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