Transgenic medaka overexpressing a melanin-concentrating hormone exhibit lightened body color but no remarkable abnormality
Melanin-concentrating hormone (MCH) is a cyclic heptadecapeptide that concentrates melanin granules in the melanophores and lightens the body color of a fish. To investigate the utility of MCH as a reporter gene, a transgenic medaka strain overexpressing the MCH gene was established and its phenotyp...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2001-11, Vol.3 (6), p.536-543 |
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| creator | Kinoshita, M Morita, T Toyohara, H Hirata, T Sakaguchi, M Ono, M Inoue, K Wakamatsu, Y Ozato, K |
| description | Melanin-concentrating hormone (MCH) is a cyclic heptadecapeptide that concentrates melanin granules in the melanophores and lightens the body color of a fish. To investigate the utility of MCH as a reporter gene, a transgenic medaka strain overexpressing the MCH gene was established and its phenotypic features were examined. The salmon MCH gene driven by cytomegalovirus promoter was injected into 100 fertilized eggs of the HNI-1 medaka strain, which exhibits black body color. One F(0) female transmitted the transgene and a lightened body color phenotype to the F(1) generation. A homozygous transgenic strain was established by crossing F(2) fish homozygous for the transgene. Expression of the transgene was detected in several organs by Northern blotting. The melanin granules of transgenics were highly shrunk. Bioassay using scales confirmed the secretion of MCH into blood, and the MCH concentration was estimated between 0.5 and 5 microM. Development, growth, feeding behavior, and reproduction of transgenics did not differ significantly among transgenic and nontransgenic siblings. The result whereby enhanced MCH expression induced a change in body color, but no remarkable abnormality, suggests the usefulness of MCH as a novel reporter gene with unique features. |
| doi_str_mv | 10.1007/s10126-001-0061-Y |
| format | Article |
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To investigate the utility of MCH as a reporter gene, a transgenic medaka strain overexpressing the MCH gene was established and its phenotypic features were examined. The salmon MCH gene driven by cytomegalovirus promoter was injected into 100 fertilized eggs of the HNI-1 medaka strain, which exhibits black body color. One F(0) female transmitted the transgene and a lightened body color phenotype to the F(1) generation. A homozygous transgenic strain was established by crossing F(2) fish homozygous for the transgene. Expression of the transgene was detected in several organs by Northern blotting. The melanin granules of transgenics were highly shrunk. Bioassay using scales confirmed the secretion of MCH into blood, and the MCH concentration was estimated between 0.5 and 5 microM. Development, growth, feeding behavior, and reproduction of transgenics did not differ significantly among transgenic and nontransgenic siblings. The result whereby enhanced MCH expression induced a change in body color, but no remarkable abnormality, suggests the usefulness of MCH as a novel reporter gene with unique features.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-001-0061-Y</identifier><identifier>PMID: 14961326</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Bio-assays ; Bioassays ; Blood ; Blood levels ; Body organs ; Brackish ; Chromatophores ; Color ; Colour ; Eggs ; Feeding behavior ; Feeding habits ; Fish ; Freshwater ; Freshwater fishes ; Genetics ; Granular materials ; Hormones ; Marine ; Melanin ; Melanin-concentrating hormone ; melanophores ; Northern blotting ; Organs ; Oryzias latipes ; Phenotypes ; Promoters ; Reporter gene ; Salmon ; Scales ; Secretion ; Siblings ; transgenic fish</subject><ispartof>Marine biotechnology (New York, N.Y.), 2001-11, Vol.3 (6), p.536-543</ispartof><rights>Springer-Verlag New York Inc. 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-6449b5e25d267c2cbbc2bef3a43c5f05fd08f27b846adb6443ad44467d3b7f7a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14961326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kinoshita, M</creatorcontrib><creatorcontrib>Morita, T</creatorcontrib><creatorcontrib>Toyohara, H</creatorcontrib><creatorcontrib>Hirata, T</creatorcontrib><creatorcontrib>Sakaguchi, M</creatorcontrib><creatorcontrib>Ono, M</creatorcontrib><creatorcontrib>Inoue, K</creatorcontrib><creatorcontrib>Wakamatsu, Y</creatorcontrib><creatorcontrib>Ozato, K</creatorcontrib><title>Transgenic medaka overexpressing a melanin-concentrating hormone exhibit lightened body color but no remarkable abnormality</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol (NY)</addtitle><description>Melanin-concentrating hormone (MCH) is a cyclic heptadecapeptide that concentrates melanin granules in the melanophores and lightens the body color of a fish. To investigate the utility of MCH as a reporter gene, a transgenic medaka strain overexpressing the MCH gene was established and its phenotypic features were examined. The salmon MCH gene driven by cytomegalovirus promoter was injected into 100 fertilized eggs of the HNI-1 medaka strain, which exhibits black body color. One F(0) female transmitted the transgene and a lightened body color phenotype to the F(1) generation. A homozygous transgenic strain was established by crossing F(2) fish homozygous for the transgene. Expression of the transgene was detected in several organs by Northern blotting. The melanin granules of transgenics were highly shrunk. Bioassay using scales confirmed the secretion of MCH into blood, and the MCH concentration was estimated between 0.5 and 5 microM. Development, growth, feeding behavior, and reproduction of transgenics did not differ significantly among transgenic and nontransgenic siblings. The result whereby enhanced MCH expression induced a change in body color, but no remarkable abnormality, suggests the usefulness of MCH as a novel reporter gene with unique features.</description><subject>Bio-assays</subject><subject>Bioassays</subject><subject>Blood</subject><subject>Blood levels</subject><subject>Body organs</subject><subject>Brackish</subject><subject>Chromatophores</subject><subject>Color</subject><subject>Colour</subject><subject>Eggs</subject><subject>Feeding behavior</subject><subject>Feeding habits</subject><subject>Fish</subject><subject>Freshwater</subject><subject>Freshwater fishes</subject><subject>Genetics</subject><subject>Granular materials</subject><subject>Hormones</subject><subject>Marine</subject><subject>Melanin</subject><subject>Melanin-concentrating hormone</subject><subject>melanophores</subject><subject>Northern blotting</subject><subject>Organs</subject><subject>Oryzias 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(NY)</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>3</volume><issue>6</issue><spage>536</spage><epage>543</epage><pages>536-543</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Melanin-concentrating hormone (MCH) is a cyclic heptadecapeptide that concentrates melanin granules in the melanophores and lightens the body color of a fish. To investigate the utility of MCH as a reporter gene, a transgenic medaka strain overexpressing the MCH gene was established and its phenotypic features were examined. The salmon MCH gene driven by cytomegalovirus promoter was injected into 100 fertilized eggs of the HNI-1 medaka strain, which exhibits black body color. One F(0) female transmitted the transgene and a lightened body color phenotype to the F(1) generation. A homozygous transgenic strain was established by crossing F(2) fish homozygous for the transgene. Expression of the transgene was detected in several organs by Northern blotting. The melanin granules of transgenics were highly shrunk. Bioassay using scales confirmed the secretion of MCH into blood, and the MCH concentration was estimated between 0.5 and 5 microM. Development, growth, feeding behavior, and reproduction of transgenics did not differ significantly among transgenic and nontransgenic siblings. The result whereby enhanced MCH expression induced a change in body color, but no remarkable abnormality, suggests the usefulness of MCH as a novel reporter gene with unique features.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>14961326</pmid><doi>10.1007/s10126-001-0061-Y</doi><tpages>8</tpages></addata></record> |
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| ispartof | Marine biotechnology (New York, N.Y.), 2001-11, Vol.3 (6), p.536-543 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Bio-assays Bioassays Blood Blood levels Body organs Brackish Chromatophores Color Colour Eggs Feeding behavior Feeding habits Fish Freshwater Freshwater fishes Genetics Granular materials Hormones Marine Melanin Melanin-concentrating hormone melanophores Northern blotting Organs Oryzias latipes Phenotypes Promoters Reporter gene Salmon Scales Secretion Siblings transgenic fish |
| title | Transgenic medaka overexpressing a melanin-concentrating hormone exhibit lightened body color but no remarkable abnormality |
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