Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene
Biochemical and histological analyses were used to study the number and size of cells (cellularity) in tissues of fast-growing, genetically modified Arctic char (Salvelinus alpinus L.), overexpressing sockeye salmon (Oncorhynchus nerka) growth hormone gene (OnGH1). DNA contents of muscle, heart, and...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2001-03, Vol.3 (2), p.188-197 |
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| creator | Pitkänen, T I Xie, S Q Krasnov, A Mason, P S Mölsä, H Stickland, N C |
| description | Biochemical and histological analyses were used to study the number and size of cells (cellularity) in tissues of fast-growing, genetically modified Arctic char (Salvelinus alpinus L.), overexpressing sockeye salmon (Oncorhynchus nerka) growth hormone gene (OnGH1). DNA contents of muscle, heart, and liver were compared in transformed, sibling (age control) and 1 year older (size control) char. Total white muscle cross-sectional area, white muscle fiber number, and total nuclei number within the muscle tissue were determined from one complete half-section of each fish. The analyzed tissues responded differently to growth hormone overproduction. In muscle tissue of OnGH1-transformed char, the enhanced growth was clearly associated with proliferation of muscle cells (hyperplasia), whereas in heart tissue both cell proliferation and increase in cell size (hypertrophy) were enhanced. The relative DNA concentration in the liver of transformed char was significantly greater than that of control fish, suggesting reduction in size of hepatic cells. |
| doi_str_mv | 10.1007/s101260000060 |
| format | Article |
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DNA contents of muscle, heart, and liver were compared in transformed, sibling (age control) and 1 year older (size control) char. Total white muscle cross-sectional area, white muscle fiber number, and total nuclei number within the muscle tissue were determined from one complete half-section of each fish. The analyzed tissues responded differently to growth hormone overproduction. In muscle tissue of OnGH1-transformed char, the enhanced growth was clearly associated with proliferation of muscle cells (hyperplasia), whereas in heart tissue both cell proliferation and increase in cell size (hypertrophy) were enhanced. The relative DNA concentration in the liver of transformed char was significantly greater than that of control fish, suggesting reduction in size of hepatic cells.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s101260000060</identifier><identifier>PMID: 14961382</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Brackish ; Cell growth ; Cell proliferation ; Cell size ; Cells ; Control ; Deoxyribonucleic acid ; DNA ; Fish ; Freshwater ; Freshwater fishes ; Genetic modification ; Growth hormones ; Heart ; Hormones ; Hyperplasia ; Hypertrophy ; Liver ; Marine ; Nuclei ; Nucleus ; Oncorhynchus nerka ; OnGH1 gene ; Proliferation ; Recombinants ; Salmon ; Salvelinus alpinus ; Tissue ; Tissues ; transgenic fish</subject><ispartof>Marine biotechnology (New York, N.Y.), 2001-03, Vol.3 (2), p.188-197</ispartof><rights>Springer-Verlag New York Inc. 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-7896da4426492a54704f935db1675b80aff04521871f07d5af47442b31b852a93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14961382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pitkänen, T I</creatorcontrib><creatorcontrib>Xie, S Q</creatorcontrib><creatorcontrib>Krasnov, A</creatorcontrib><creatorcontrib>Mason, P S</creatorcontrib><creatorcontrib>Mölsä, H</creatorcontrib><creatorcontrib>Stickland, N C</creatorcontrib><title>Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol (NY)</addtitle><description>Biochemical and histological analyses were used to study the number and size of cells (cellularity) in tissues of fast-growing, genetically modified Arctic char (Salvelinus alpinus L.), overexpressing sockeye salmon (Oncorhynchus nerka) growth hormone gene (OnGH1). DNA contents of muscle, heart, and liver were compared in transformed, sibling (age control) and 1 year older (size control) char. Total white muscle cross-sectional area, white muscle fiber number, and total nuclei number within the muscle tissue were determined from one complete half-section of each fish. The analyzed tissues responded differently to growth hormone overproduction. In muscle tissue of OnGH1-transformed char, the enhanced growth was clearly associated with proliferation of muscle cells (hyperplasia), whereas in heart tissue both cell proliferation and increase in cell size (hypertrophy) were enhanced. The relative DNA concentration in the liver of transformed char was significantly greater than that of control fish, suggesting reduction in size of hepatic cells.</description><subject>Brackish</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cell size</subject><subject>Cells</subject><subject>Control</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fish</subject><subject>Freshwater</subject><subject>Freshwater fishes</subject><subject>Genetic modification</subject><subject>Growth hormones</subject><subject>Heart</subject><subject>Hormones</subject><subject>Hyperplasia</subject><subject>Hypertrophy</subject><subject>Liver</subject><subject>Marine</subject><subject>Nuclei</subject><subject>Nucleus</subject><subject>Oncorhynchus nerka</subject><subject>OnGH1 gene</subject><subject>Proliferation</subject><subject>Recombinants</subject><subject>Salmon</subject><subject>Salvelinus 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| ispartof | Marine biotechnology (New York, N.Y.), 2001-03, Vol.3 (2), p.188-197 |
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| subjects | Brackish Cell growth Cell proliferation Cell size Cells Control Deoxyribonucleic acid DNA Fish Freshwater Freshwater fishes Genetic modification Growth hormones Heart Hormones Hyperplasia Hypertrophy Liver Marine Nuclei Nucleus Oncorhynchus nerka OnGH1 gene Proliferation Recombinants Salmon Salvelinus alpinus Tissue Tissues transgenic fish |
| title | Changes in tissue cellularity are associated with growth enhancement in genetically modified arctic char (Salvelinus alpinus L.) carrying recombinant growth hormone gene |
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