Immunostaining of small-cell carcinoma: mistaken identity?
Sir-We can say with certainty that the cells in question were small-cell carcinoma cells and not red blood cells. First, the renal glomeruli in this case contained only a few red cells, as shown by standard haematoxylin and eosin stains, but did contain many nucleated cells similar to those in the p...
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| Veröffentlicht in: | The Lancet (British edition) 2003-11, Vol.362 (9395), p.1583-1583 |
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| description | Sir-We can say with certainty that the cells in question were small-cell carcinoma cells and not red blood cells. First, the renal glomeruli in this case contained only a few red cells, as shown by standard haematoxylin and eosin stains, but did contain many nucleated cells similar to those in the primary lung cancer. Second, the appropriate blocking procedures were used to prepare the immunoperoxidase preparations. Third, the cytoplasm of the cells in the glomeruli stained positive for cytokeratin, which is not a component of red cells and is not found in the tumour-cell nuclei. Fourth, the cells in the glomeruli also stained positive for TTF-1 when alkaline phosphatase was used in place of peroxidase. Fifth, staining for haemoglobin was negative. |
| doi_str_mv | 10.1016/S0140-6736(03)14754-X |
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First, the renal glomeruli in this case contained only a few red cells, as shown by standard haematoxylin and eosin stains, but did contain many nucleated cells similar to those in the primary lung cancer. Second, the appropriate blocking procedures were used to prepare the immunoperoxidase preparations. Third, the cytoplasm of the cells in the glomeruli stained positive for cytokeratin, which is not a component of red cells and is not found in the tumour-cell nuclei. Fourth, the cells in the glomeruli also stained positive for TTF-1 when alkaline phosphatase was used in place of peroxidase. Fifth, staining for haemoglobin was negative.</description><identifier>ISSN: 0140-6736</identifier><identifier>EISSN: 1474-547X</identifier><identifier>DOI: 10.1016/S0140-6736(03)14754-X</identifier><identifier>PMID: 14615122</identifier><identifier>CODEN: LANCAO</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Alkaline Phosphatase ; Biomarkers, Tumor - analysis ; Cancer ; Carcinoma, Small Cell - chemistry ; Carcinoma, Small Cell - diagnosis ; Carcinoma, Small Cell - secondary ; Diagnostic Errors ; Erythrocytes - chemistry ; Erythrocytes - enzymology ; Homeodomain Proteins - analysis ; Horseradish Peroxidase ; Humans ; Immunoenzyme Techniques - methods ; Immunohistochemistry ; Kidney Glomerulus - chemistry ; Kidney Neoplasms - chemistry ; Kidney Neoplasms - diagnosis ; Kidney Neoplasms - secondary ; Lung cancer ; Lung Neoplasms - chemistry ; Lung Neoplasms - diagnosis ; Lung Neoplasms - pathology ; Neoplastic Cells, Circulating - chemistry ; Nuclear Proteins - analysis ; Photomicrography ; Thyroid Nuclear Factor 1 ; Transcription factors ; Transcription Factors - analysis</subject><ispartof>The Lancet (British edition), 2003-11, Vol.362 (9395), p.1583-1583</ispartof><rights>2003 Elsevier Ltd</rights><rights>Copyright Lancet Ltd. Nov 8, 2003</rights><rights>Copyright Elsevier Limited Nov 8, 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c411t-bff85abf5836373f76dea8a1872779622b8f8d2374e34742f78f877e79378adb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014067360314754X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14615122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmitt, Roland</creatorcontrib><creatorcontrib>Weichert, Wilko</creatorcontrib><title>Immunostaining of small-cell carcinoma: mistaken identity?</title><title>The Lancet (British edition)</title><addtitle>Lancet</addtitle><description>Sir-We can say with certainty that the cells in question were small-cell carcinoma cells and not red blood cells. First, the renal glomeruli in this case contained only a few red cells, as shown by standard haematoxylin and eosin stains, but did contain many nucleated cells similar to those in the primary lung cancer. Second, the appropriate blocking procedures were used to prepare the immunoperoxidase preparations. Third, the cytoplasm of the cells in the glomeruli stained positive for cytokeratin, which is not a component of red cells and is not found in the tumour-cell nuclei. Fourth, the cells in the glomeruli also stained positive for TTF-1 when alkaline phosphatase was used in place of peroxidase. Fifth, staining for haemoglobin was negative.</description><subject>Alkaline Phosphatase</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Cancer</subject><subject>Carcinoma, Small Cell - chemistry</subject><subject>Carcinoma, Small Cell - diagnosis</subject><subject>Carcinoma, Small Cell - secondary</subject><subject>Diagnostic Errors</subject><subject>Erythrocytes - chemistry</subject><subject>Erythrocytes - enzymology</subject><subject>Homeodomain Proteins - analysis</subject><subject>Horseradish Peroxidase</subject><subject>Humans</subject><subject>Immunoenzyme Techniques - methods</subject><subject>Immunohistochemistry</subject><subject>Kidney Glomerulus - chemistry</subject><subject>Kidney Neoplasms - chemistry</subject><subject>Kidney Neoplasms - diagnosis</subject><subject>Kidney Neoplasms - secondary</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - chemistry</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Lung Neoplasms - pathology</subject><subject>Neoplastic Cells, Circulating - chemistry</subject><subject>Nuclear Proteins - analysis</subject><subject>Photomicrography</subject><subject>Thyroid Nuclear Factor 1</subject><subject>Transcription factors</subject><subject>Transcription Factors - analysis</subject><issn>0140-6736</issn><issn>1474-547X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkF1r2zAUQMXoWNJsP2HFdFC2B2_6sq6cl1JK1wYCfWgHeROyLRWlttxK9iD_vsoHGxRKnyTBuVeHg9BXgn8STMSvO0w4zgUw8R2zH4RDwfPVBzRNN54XHFZHaPoPmaDjGNcYYy5w8QlNCBekIJRO0XzRdaPv46Cdd_4h620WO922eW3aNqt1qJ3vOz3POpeYR-Mz1xg_uGFz_hl9tLqN5svhnKE_v6_uL2_y5e314vJimdeckCGvrJWFrmwhmWDALIjGaKmJBApQCkoraWVDGXDDkju1kN4ABkoGUjcVm6Gz_d6n0D-PJg4quWz1tDf9GBUQBkJSksBvr8B1Pwaf3BTFoiwLyZPADJ2-RZGyxBwo5wkq9lAd-hiDseopuE6HjSJYbfurXX-1jaswU7v-apXmTg7Lx6ozzf-pQ_AEnO8Bk4r9dSaoWDvja9O4YOpBNb1754sXD16SuA</recordid><startdate>20031108</startdate><enddate>20031108</enddate><creator>Schmitt, Roland</creator><creator>Weichert, Wilko</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0TT</scope><scope>0TZ</scope><scope>0U~</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88C</scope><scope>88E</scope><scope>88G</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8C2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AN0</scope><scope>ASE</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FPQ</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K6X</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>KB~</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20031108</creationdate><title>Immunostaining of small-cell carcinoma: mistaken identity?</title><author>Schmitt, Roland ; Weichert, Wilko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-bff85abf5836373f76dea8a1872779622b8f8d2374e34742f78f877e79378adb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Alkaline Phosphatase</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Cancer</topic><topic>Carcinoma, Small Cell - chemistry</topic><topic>Carcinoma, Small Cell - diagnosis</topic><topic>Carcinoma, Small Cell - secondary</topic><topic>Diagnostic Errors</topic><topic>Erythrocytes - chemistry</topic><topic>Erythrocytes - enzymology</topic><topic>Homeodomain Proteins - analysis</topic><topic>Horseradish Peroxidase</topic><topic>Humans</topic><topic>Immunoenzyme Techniques - methods</topic><topic>Immunohistochemistry</topic><topic>Kidney Glomerulus - chemistry</topic><topic>Kidney Neoplasms - chemistry</topic><topic>Kidney Neoplasms - diagnosis</topic><topic>Kidney Neoplasms - secondary</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - chemistry</topic><topic>Lung Neoplasms - diagnosis</topic><topic>Lung Neoplasms - pathology</topic><topic>Neoplastic Cells, Circulating - chemistry</topic><topic>Nuclear Proteins - analysis</topic><topic>Photomicrography</topic><topic>Thyroid Nuclear Factor 1</topic><topic>Transcription factors</topic><topic>Transcription Factors - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmitt, Roland</creatorcontrib><creatorcontrib>Weichert, Wilko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>News PRO</collection><collection>Pharma and Biotech Premium PRO</collection><collection>Global News & ABI/Inform Professional</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Lancet Titles</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>British Nursing Index</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>British Nursing Index</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Newsstand Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>The Lancet (British edition)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmitt, Roland</au><au>Weichert, Wilko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunostaining of small-cell carcinoma: mistaken identity?</atitle><jtitle>The Lancet (British edition)</jtitle><addtitle>Lancet</addtitle><date>2003-11-08</date><risdate>2003</risdate><volume>362</volume><issue>9395</issue><spage>1583</spage><epage>1583</epage><pages>1583-1583</pages><issn>0140-6736</issn><eissn>1474-547X</eissn><coden>LANCAO</coden><abstract>Sir-We can say with certainty that the cells in question were small-cell carcinoma cells and not red blood cells. First, the renal glomeruli in this case contained only a few red cells, as shown by standard haematoxylin and eosin stains, but did contain many nucleated cells similar to those in the primary lung cancer. Second, the appropriate blocking procedures were used to prepare the immunoperoxidase preparations. Third, the cytoplasm of the cells in the glomeruli stained positive for cytokeratin, which is not a component of red cells and is not found in the tumour-cell nuclei. Fourth, the cells in the glomeruli also stained positive for TTF-1 when alkaline phosphatase was used in place of peroxidase. Fifth, staining for haemoglobin was negative.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>14615122</pmid><doi>10.1016/S0140-6736(03)14754-X</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaline Phosphatase Biomarkers, Tumor - analysis Cancer Carcinoma, Small Cell - chemistry Carcinoma, Small Cell - diagnosis Carcinoma, Small Cell - secondary Diagnostic Errors Erythrocytes - chemistry Erythrocytes - enzymology Homeodomain Proteins - analysis Horseradish Peroxidase Humans Immunoenzyme Techniques - methods Immunohistochemistry Kidney Glomerulus - chemistry Kidney Neoplasms - chemistry Kidney Neoplasms - diagnosis Kidney Neoplasms - secondary Lung cancer Lung Neoplasms - chemistry Lung Neoplasms - diagnosis Lung Neoplasms - pathology Neoplastic Cells, Circulating - chemistry Nuclear Proteins - analysis Photomicrography Thyroid Nuclear Factor 1 Transcription factors Transcription Factors - analysis |
| title | Immunostaining of small-cell carcinoma: mistaken identity? |
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