Regulatory effect of castration on endothelins, their receptors and endothelin‐converting enzyme in rat seminal vesicle
OBJECTIVE To investigate the effects of castration on the expression of endothelins (ETs), ET receptors and ET converting enzyme‐1 (ECE‐1) in the rat seminal vesicle (RSV). MATERIALS AND METHODS Sprague‐Dawley rats (3 months old) were surgically castrated or sham‐operated, and then killed 7 days aft...
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| Veröffentlicht in: | BJU international 2003-11, Vol.92 (7), p.803-809 |
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| creator | Takahashi, W. Yono, M. Wada, Y. Ikeda, K. Weiss, R.M. Latifpour, J. |
| description | OBJECTIVE
To investigate the effects of castration on the expression of endothelins (ETs), ET receptors and ET converting enzyme‐1 (ECE‐1) in the rat seminal vesicle (RSV).
MATERIALS AND METHODS
Sprague‐Dawley rats (3 months old) were surgically castrated or sham‐operated, and then killed 7 days after surgery. Biochemical and pharmacological properties and the location of ET receptors in the RSV were determined by a series of binding experiments with [125I]ET‐1, using membrane particulates and slide‐mounted frozen sections of RSV. Expression levels of ETA and ETB receptor subtypes, ET‐1, ET‐3 and ECE‐1 mRNAs were assessed by relative multiplex reverse‐transcription polymerase chain reaction (RT‐PCR).
RESULTS
The density of total ET receptors increased significantly in the seminal vesicle of the castrated rat. The predominance of the ETA receptor subtype in the RSV did not change with castration. Autoradiographic studies showed the presence of ET receptors on the smooth muscle and epithelium of the RSV. In addition, RT‐PCR showed an up‐regulation in the expression of ETA and ETB receptor subtypes, ET‐1 and ECE‐1 mRNAs in the seminal vesicle of the castrated rat. However, castration caused no significant change in the expression levels of ET‐3 mRNA.
CONCLUSION
These findings suggest a regulatory role for testosterone in the expression of the ET receptor system in the RSV. |
| doi_str_mv | 10.1046/j.1464-410X.2003.04466.x |
| format | Article |
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To investigate the effects of castration on the expression of endothelins (ETs), ET receptors and ET converting enzyme‐1 (ECE‐1) in the rat seminal vesicle (RSV).
MATERIALS AND METHODS
Sprague‐Dawley rats (3 months old) were surgically castrated or sham‐operated, and then killed 7 days after surgery. Biochemical and pharmacological properties and the location of ET receptors in the RSV were determined by a series of binding experiments with [125I]ET‐1, using membrane particulates and slide‐mounted frozen sections of RSV. Expression levels of ETA and ETB receptor subtypes, ET‐1, ET‐3 and ECE‐1 mRNAs were assessed by relative multiplex reverse‐transcription polymerase chain reaction (RT‐PCR).
RESULTS
The density of total ET receptors increased significantly in the seminal vesicle of the castrated rat. The predominance of the ETA receptor subtype in the RSV did not change with castration. Autoradiographic studies showed the presence of ET receptors on the smooth muscle and epithelium of the RSV. In addition, RT‐PCR showed an up‐regulation in the expression of ETA and ETB receptor subtypes, ET‐1 and ECE‐1 mRNAs in the seminal vesicle of the castrated rat. However, castration caused no significant change in the expression levels of ET‐3 mRNA.
CONCLUSION
These findings suggest a regulatory role for testosterone in the expression of the ET receptor system in the RSV.</description><identifier>ISSN: 1464-4096</identifier><identifier>EISSN: 1464-410X</identifier><identifier>DOI: 10.1046/j.1464-410X.2003.04466.x</identifier><identifier>PMID: 14616470</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Aspartic Acid Endopeptidases - metabolism ; Autoradiography - methods ; Biological and medical sciences ; Castration ; endothelin ; Endothelin-Converting Enzymes ; Endothelins - metabolism ; Fundamental and applied biological sciences. Psychology ; Male ; Mammalian male genital system ; Metalloendopeptidases ; Morphology. Physiology ; rat ; Rats ; Rats, Sprague-Dawley ; Receptors, Endothelin - metabolism ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RT‐PCR ; seminal vesicle ; Seminal Vesicles - metabolism ; Vertebrates: reproduction</subject><ispartof>BJU international, 2003-11, Vol.92 (7), p.803-809</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3956-9b855d8afd13f4cbf628445e921c051800c9387415ada49b8109a66fe26c5fb43</citedby><cites>FETCH-LOGICAL-c3956-9b855d8afd13f4cbf628445e921c051800c9387415ada49b8109a66fe26c5fb43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1464-410X.2003.04466.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1464-410X.2003.04466.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15281008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14616470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takahashi, W.</creatorcontrib><creatorcontrib>Yono, M.</creatorcontrib><creatorcontrib>Wada, Y.</creatorcontrib><creatorcontrib>Ikeda, K.</creatorcontrib><creatorcontrib>Weiss, R.M.</creatorcontrib><creatorcontrib>Latifpour, J.</creatorcontrib><title>Regulatory effect of castration on endothelins, their receptors and endothelin‐converting enzyme in rat seminal vesicle</title><title>BJU international</title><addtitle>BJU Int</addtitle><description>OBJECTIVE
To investigate the effects of castration on the expression of endothelins (ETs), ET receptors and ET converting enzyme‐1 (ECE‐1) in the rat seminal vesicle (RSV).
MATERIALS AND METHODS
Sprague‐Dawley rats (3 months old) were surgically castrated or sham‐operated, and then killed 7 days after surgery. Biochemical and pharmacological properties and the location of ET receptors in the RSV were determined by a series of binding experiments with [125I]ET‐1, using membrane particulates and slide‐mounted frozen sections of RSV. Expression levels of ETA and ETB receptor subtypes, ET‐1, ET‐3 and ECE‐1 mRNAs were assessed by relative multiplex reverse‐transcription polymerase chain reaction (RT‐PCR).
RESULTS
The density of total ET receptors increased significantly in the seminal vesicle of the castrated rat. The predominance of the ETA receptor subtype in the RSV did not change with castration. Autoradiographic studies showed the presence of ET receptors on the smooth muscle and epithelium of the RSV. In addition, RT‐PCR showed an up‐regulation in the expression of ETA and ETB receptor subtypes, ET‐1 and ECE‐1 mRNAs in the seminal vesicle of the castrated rat. However, castration caused no significant change in the expression levels of ET‐3 mRNA.
CONCLUSION
These findings suggest a regulatory role for testosterone in the expression of the ET receptor system in the RSV.</description><subject>Animals</subject><subject>Aspartic Acid Endopeptidases - metabolism</subject><subject>Autoradiography - methods</subject><subject>Biological and medical sciences</subject><subject>Castration</subject><subject>endothelin</subject><subject>Endothelin-Converting Enzymes</subject><subject>Endothelins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Metalloendopeptidases</subject><subject>Morphology. Physiology</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Endothelin - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RT‐PCR</subject><subject>seminal vesicle</subject><subject>Seminal Vesicles - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>1464-4096</issn><issn>1464-410X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM9O3DAQxq2qqFDaV0C-wKkb7MR2kgMHiqCAkJBQV-rN8jrjxSvHWewsEE59hD5jn6QOu_w5VrI0o_Hvm_n0IYQpyShh4nCRUSbYhFHyK8sJKTLCmBDZ4we08_rx8aUntdhGn2NcEJIGgn9C26lSwUqyg4YbmK-c6rswYDAGdI87g7WKfVC97TxOD3zT9bfgrI_fcGpswAE0LJMoYuWbd8Df33905-8h9NbP0_xpaAFbj9MyHKG1Xjl8D9FqB1_QllEuwtdN3UXTs9OfJ-eTq-sfFyfHVxNd1FxM6lnFeVMp09DCMD0zIq8Y41DnVBNOK0J0XVQlo1w1iiWakloJYSAXmpsZK3bRwXrvMnR3K4i9bG3U4Jzy0K2iLGlR8rKoElitQR26GAMYuQy2VWGQlMgxdrmQY6JyTFeOscvn2OVjku5tbqxmLTRvwk3OCdjfACpq5UxQXtv4xvE8-Sajh6M192AdDP9tQH6_nI5d8Q-EGaFt</recordid><startdate>200311</startdate><enddate>200311</enddate><creator>Takahashi, W.</creator><creator>Yono, M.</creator><creator>Wada, Y.</creator><creator>Ikeda, K.</creator><creator>Weiss, R.M.</creator><creator>Latifpour, J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200311</creationdate><title>Regulatory effect of castration on endothelins, their receptors and endothelin‐converting enzyme in rat seminal vesicle</title><author>Takahashi, W. ; Yono, M. ; Wada, Y. ; Ikeda, K. ; Weiss, R.M. ; Latifpour, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3956-9b855d8afd13f4cbf628445e921c051800c9387415ada49b8109a66fe26c5fb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Aspartic Acid Endopeptidases - metabolism</topic><topic>Autoradiography - methods</topic><topic>Biological and medical sciences</topic><topic>Castration</topic><topic>endothelin</topic><topic>Endothelin-Converting Enzymes</topic><topic>Endothelins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Metalloendopeptidases</topic><topic>Morphology. Physiology</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Endothelin - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RT‐PCR</topic><topic>seminal vesicle</topic><topic>Seminal Vesicles - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takahashi, W.</creatorcontrib><creatorcontrib>Yono, M.</creatorcontrib><creatorcontrib>Wada, Y.</creatorcontrib><creatorcontrib>Ikeda, K.</creatorcontrib><creatorcontrib>Weiss, R.M.</creatorcontrib><creatorcontrib>Latifpour, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>BJU international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takahashi, W.</au><au>Yono, M.</au><au>Wada, Y.</au><au>Ikeda, K.</au><au>Weiss, R.M.</au><au>Latifpour, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulatory effect of castration on endothelins, their receptors and endothelin‐converting enzyme in rat seminal vesicle</atitle><jtitle>BJU international</jtitle><addtitle>BJU Int</addtitle><date>2003-11</date><risdate>2003</risdate><volume>92</volume><issue>7</issue><spage>803</spage><epage>809</epage><pages>803-809</pages><issn>1464-4096</issn><eissn>1464-410X</eissn><abstract>OBJECTIVE
To investigate the effects of castration on the expression of endothelins (ETs), ET receptors and ET converting enzyme‐1 (ECE‐1) in the rat seminal vesicle (RSV).
MATERIALS AND METHODS
Sprague‐Dawley rats (3 months old) were surgically castrated or sham‐operated, and then killed 7 days after surgery. Biochemical and pharmacological properties and the location of ET receptors in the RSV were determined by a series of binding experiments with [125I]ET‐1, using membrane particulates and slide‐mounted frozen sections of RSV. Expression levels of ETA and ETB receptor subtypes, ET‐1, ET‐3 and ECE‐1 mRNAs were assessed by relative multiplex reverse‐transcription polymerase chain reaction (RT‐PCR).
RESULTS
The density of total ET receptors increased significantly in the seminal vesicle of the castrated rat. The predominance of the ETA receptor subtype in the RSV did not change with castration. Autoradiographic studies showed the presence of ET receptors on the smooth muscle and epithelium of the RSV. In addition, RT‐PCR showed an up‐regulation in the expression of ETA and ETB receptor subtypes, ET‐1 and ECE‐1 mRNAs in the seminal vesicle of the castrated rat. However, castration caused no significant change in the expression levels of ET‐3 mRNA.
CONCLUSION
These findings suggest a regulatory role for testosterone in the expression of the ET receptor system in the RSV.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>14616470</pmid><doi>10.1046/j.1464-410X.2003.04466.x</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Aspartic Acid Endopeptidases - metabolism Autoradiography - methods Biological and medical sciences Castration endothelin Endothelin-Converting Enzymes Endothelins - metabolism Fundamental and applied biological sciences. Psychology Male Mammalian male genital system Metalloendopeptidases Morphology. Physiology rat Rats Rats, Sprague-Dawley Receptors, Endothelin - metabolism Reverse Transcriptase Polymerase Chain Reaction - methods RT‐PCR seminal vesicle Seminal Vesicles - metabolism Vertebrates: reproduction |
| title | Regulatory effect of castration on endothelins, their receptors and endothelin‐converting enzyme in rat seminal vesicle |
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