Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica

Polyamines are required for cellular growth and differentiation. In mammals and fungi they are synthesized via a pathway involving ornithine decarboxylase (ODC), which transforms ornithine into putrescine. We have cloned and disrupted the gene coding for ODC in Yarrowia lipolytica to analyze the rol...

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Veröffentlicht in:	FEMS yeast research 2001-12, Vol.1 (3), p.195-204
Hauptverfasser:	Jiménez-Bremont, Juan Francisco, Ruiz-Herrera, José, Dominguez, Angel
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences auxotrophs Base Sequence Cloning, Molecular complementary DNA Culture Media dimorphism Fungal dimorphism Fungal Proteins - genetics Fungal Proteins - metabolism Gene Deletion Gene Expression Regulation, Fungal gene targeting genes mammals Molecular Sequence Data mycelium Northern blotting ornithine Ornithine decarboxylase Ornithine Decarboxylase - genetics Ornithine Decarboxylase - metabolism Polyamine polyamines Polyamines - metabolism putrescine rapid amplification of cDNA ends Sequence Analysis, DNA Yarrowia - enzymology Yarrowia - genetics Yarrowia - growth & development Yarrowia lipolytica yeasts YlODC gene
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