Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica
Polyamines are required for cellular growth and differentiation. In mammals and fungi they are synthesized via a pathway involving ornithine decarboxylase (ODC), which transforms ornithine into putrescine. We have cloned and disrupted the gene coding for ODC in Yarrowia lipolytica to analyze the rol...
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| Veröffentlicht in: | FEMS yeast research 2001-12, Vol.1 (3), p.195-204 |
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| creator | Jiménez-Bremont, Juan Francisco Ruiz-Herrera, José Dominguez, Angel |
| description | Polyamines are required for cellular growth and differentiation. In mammals and fungi they are synthesized via a pathway involving ornithine decarboxylase (ODC), which transforms ornithine into putrescine. We have cloned and disrupted the gene coding for ODC in Yarrowia lipolytica to analyze the role of polyamines in dimorphism of this fungus. Substrate- and cofactor-binding motifs, as well as two putative PEST boxes were identified in the amino acid sequence. A single transcript 1.7 kb in size was identified by Northern hybridization, and confirmed by rapid amplification of cDNA ends (RACE). Null mutants lacked ODC activity and behaved as polyamine auxotrophs. When low levels of polyamines were supplied to the null mutant, only yeast-like, but not mycelial growth was sustained. This phenomenon was confirmed by introduction of the YlODC gene under the control of an inducible promoter into the null mutant. |
| doi_str_mv | 10.1111/j.1567-1364.2001.tb00034.x |
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In mammals and fungi they are synthesized via a pathway involving ornithine decarboxylase (ODC), which transforms ornithine into putrescine. We have cloned and disrupted the gene coding for ODC in Yarrowia lipolytica to analyze the role of polyamines in dimorphism of this fungus. Substrate- and cofactor-binding motifs, as well as two putative PEST boxes were identified in the amino acid sequence. A single transcript 1.7 kb in size was identified by Northern hybridization, and confirmed by rapid amplification of cDNA ends (RACE). Null mutants lacked ODC activity and behaved as polyamine auxotrophs. When low levels of polyamines were supplied to the null mutant, only yeast-like, but not mycelial growth was sustained. This phenomenon was confirmed by introduction of the YlODC gene under the control of an inducible promoter into the null mutant.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1111/j.1567-1364.2001.tb00034.x</identifier><identifier>PMID: 12702344</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; auxotrophs ; Base Sequence ; Cloning, Molecular ; complementary DNA ; Culture Media ; dimorphism ; Fungal dimorphism ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Deletion ; Gene Expression Regulation, Fungal ; gene targeting ; genes ; mammals ; Molecular Sequence Data ; mycelium ; Northern blotting ; ornithine ; Ornithine decarboxylase ; Ornithine Decarboxylase - genetics ; Ornithine Decarboxylase - metabolism ; Polyamine ; polyamines ; Polyamines - metabolism ; putrescine ; rapid amplification of cDNA ends ; Sequence Analysis, DNA ; Yarrowia - enzymology ; Yarrowia - genetics ; Yarrowia - growth & development ; Yarrowia lipolytica ; yeasts ; YlODC gene</subject><ispartof>FEMS yeast research, 2001-12, Vol.1 (3), p.195-204</ispartof><rights>2001 Federation of European Microbiological Societies. 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All rights reserved. 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3185-73b01612a0ef32dbe640694d849debaba3c487b9819af37497c56ca2753733743</citedby><cites>FETCH-LOGICAL-c3185-73b01612a0ef32dbe640694d849debaba3c487b9819af37497c56ca2753733743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1567-1364.2001.tb00034.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1567-1364.2001.tb00034.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12702344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiménez-Bremont, Juan Francisco</creatorcontrib><creatorcontrib>Ruiz-Herrera, José</creatorcontrib><creatorcontrib>Dominguez, Angel</creatorcontrib><title>Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica</title><title>FEMS yeast research</title><addtitle>FEMS Yeast Res</addtitle><description>Polyamines are required for cellular growth and differentiation. In mammals and fungi they are synthesized via a pathway involving ornithine decarboxylase (ODC), which transforms ornithine into putrescine. We have cloned and disrupted the gene coding for ODC in Yarrowia lipolytica to analyze the role of polyamines in dimorphism of this fungus. Substrate- and cofactor-binding motifs, as well as two putative PEST boxes were identified in the amino acid sequence. A single transcript 1.7 kb in size was identified by Northern hybridization, and confirmed by rapid amplification of cDNA ends (RACE). Null mutants lacked ODC activity and behaved as polyamine auxotrophs. When low levels of polyamines were supplied to the null mutant, only yeast-like, but not mycelial growth was sustained. This phenomenon was confirmed by introduction of the YlODC gene under the control of an inducible promoter into the null mutant.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>auxotrophs</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>Culture Media</subject><subject>dimorphism</subject><subject>Fungal dimorphism</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Fungal</subject><subject>gene targeting</subject><subject>genes</subject><subject>mammals</subject><subject>Molecular Sequence Data</subject><subject>mycelium</subject><subject>Northern blotting</subject><subject>ornithine</subject><subject>Ornithine decarboxylase</subject><subject>Ornithine Decarboxylase - genetics</subject><subject>Ornithine Decarboxylase - metabolism</subject><subject>Polyamine</subject><subject>polyamines</subject><subject>Polyamines - metabolism</subject><subject>putrescine</subject><subject>rapid amplification of cDNA ends</subject><subject>Sequence Analysis, DNA</subject><subject>Yarrowia - enzymology</subject><subject>Yarrowia - genetics</subject><subject>Yarrowia - growth & development</subject><subject>Yarrowia lipolytica</subject><subject>yeasts</subject><subject>YlODC gene</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUtr3DAUhUVoaZJJ_0IquujOrl627C4KZfIqBAJNs5iVkO3roEG2HMluMv--cjykm5RSbaQrfefocg9CHylJaVyftynNcplQnouUEULTsSKEcJE-HaCjl6c3L-csP0THIWwjKgkp3qFDyiRhXIgjFM5M8NMwGtdj1-J76AFv7M3ZGnv4BdoGrKvg7DRCvHiYjIcO-nFGB2d3ujM9BNw6j7tdDdZoi5uos254xkyPN9p792g0tmZWjKbWJ-htG53h_X5fobuL85_rq-T65vL7-tt1UnNaZInkFaE5ZZpAy1lTQS5IXoqmEGUDla40r0Uhq7KgpW65FKWss7zWTGZc8ljzFfq0-A7ePUwQRtWZELu0ugc3BSUpl6wQ_wZpwYqcRNMV-rKAtXcheGjV4E2n_U5RouZs1FbNQ1dzAGrORu2zUU9RfLr_Zao6aP5I92FE4OsCPBoLu_-wVhebH7TMokG2GLhp-Is8eb2zD4uu1U7pe2-CurtlcfgRYFTEgf4G6LW4yQ</recordid><startdate>200112</startdate><enddate>200112</enddate><creator>Jiménez-Bremont, Juan Francisco</creator><creator>Ruiz-Herrera, José</creator><creator>Dominguez, Angel</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200112</creationdate><title>Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica</title><author>Jiménez-Bremont, Juan Francisco ; Ruiz-Herrera, José ; Dominguez, Angel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3185-73b01612a0ef32dbe640694d849debaba3c487b9819af37497c56ca2753733743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>auxotrophs</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>Culture Media</topic><topic>dimorphism</topic><topic>Fungal dimorphism</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Fungal</topic><topic>gene targeting</topic><topic>genes</topic><topic>mammals</topic><topic>Molecular Sequence Data</topic><topic>mycelium</topic><topic>Northern blotting</topic><topic>ornithine</topic><topic>Ornithine decarboxylase</topic><topic>Ornithine Decarboxylase - genetics</topic><topic>Ornithine Decarboxylase - metabolism</topic><topic>Polyamine</topic><topic>polyamines</topic><topic>Polyamines - metabolism</topic><topic>putrescine</topic><topic>rapid amplification of cDNA ends</topic><topic>Sequence Analysis, DNA</topic><topic>Yarrowia - enzymology</topic><topic>Yarrowia - genetics</topic><topic>Yarrowia - growth & development</topic><topic>Yarrowia lipolytica</topic><topic>yeasts</topic><topic>YlODC gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiménez-Bremont, Juan Francisco</creatorcontrib><creatorcontrib>Ruiz-Herrera, José</creatorcontrib><creatorcontrib>Dominguez, Angel</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiménez-Bremont, Juan Francisco</au><au>Ruiz-Herrera, José</au><au>Dominguez, Angel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2001-12</date><risdate>2001</risdate><volume>1</volume><issue>3</issue><spage>195</spage><epage>204</epage><pages>195-204</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>Polyamines are required for cellular growth and differentiation. In mammals and fungi they are synthesized via a pathway involving ornithine decarboxylase (ODC), which transforms ornithine into putrescine. We have cloned and disrupted the gene coding for ODC in Yarrowia lipolytica to analyze the role of polyamines in dimorphism of this fungus. Substrate- and cofactor-binding motifs, as well as two putative PEST boxes were identified in the amino acid sequence. A single transcript 1.7 kb in size was identified by Northern hybridization, and confirmed by rapid amplification of cDNA ends (RACE). Null mutants lacked ODC activity and behaved as polyamine auxotrophs. When low levels of polyamines were supplied to the null mutant, only yeast-like, but not mycelial growth was sustained. This phenomenon was confirmed by introduction of the YlODC gene under the control of an inducible promoter into the null mutant.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>12702344</pmid><doi>10.1111/j.1567-1364.2001.tb00034.x</doi><tpages>10</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences auxotrophs Base Sequence Cloning, Molecular complementary DNA Culture Media dimorphism Fungal dimorphism Fungal Proteins - genetics Fungal Proteins - metabolism Gene Deletion Gene Expression Regulation, Fungal gene targeting genes mammals Molecular Sequence Data mycelium Northern blotting ornithine Ornithine decarboxylase Ornithine Decarboxylase - genetics Ornithine Decarboxylase - metabolism Polyamine polyamines Polyamines - metabolism putrescine rapid amplification of cDNA ends Sequence Analysis, DNA Yarrowia - enzymology Yarrowia - genetics Yarrowia - growth & development Yarrowia lipolytica yeasts YlODC gene |
| title | Disruption of gene YlODC reveals absolute requirement of polyamines for mycelial development in Yarrowia lipolytica |
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