Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations
The level of transgene expression in crop x weed hybrids and the degree to which crop-specific genes are integrated into hybrid populations are important factors in assessing the potential ecological and agricultural risks of gene flow associated with genetic engineering. The average transgene zygos...
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| Veröffentlicht in: | Theoretical and applied genetics 2003-11, Vol.107 (8), p.1533-1540 |
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| creator | HALFHILL, M. D MILLWOOD, R. J WEISSINGER, A. K WARWICK, S. I STEWART, C. N |
| description | The level of transgene expression in crop x weed hybrids and the degree to which crop-specific genes are integrated into hybrid populations are important factors in assessing the potential ecological and agricultural risks of gene flow associated with genetic engineering. The average transgene zygosity and genetic structure of transgenic hybrid populations change with the progression of generations, and the green fluorescent protein (GFP) transgene is an ideal marker to quantify transgene expression in advancing populations. The homozygous T(1) single-locus insert GFP/ Bacillus thuringiensis (Bt) transgenic canola ( Brassica napus, cv Westar) with two copies of the transgene fluoresced twice as much as hemizygous individuals with only one copy of the transgene. These data indicate that the expression of the GFP gene was additive, and fluorescence could be used to determine zygosity status. Several hybrid generations (BC(1)F(1), BC(2)F(1)) were produced by backcrossing various GFP/Bt transgenic canola ( B. napus, cv Westar) and birdseed rape ( Brassica rapa) hybrid generations onto B. rapa. Intercrossed generations (BC(2)F(2) Bulk) were generated by crossing BC(2)F(1) individuals in the presence of a pollinating insect ( Musca domestica L.). The ploidy of plants in the BC(2)F(2) Bulk hybrid generation was identical to the weedy parental species, B. rapa. AFLP analysis was used to quantify the degree of B. napus introgression into multiple backcross hybrid generations with B. rapa. The F(1) hybrid generations contained 95-97% of the B. napus-specific AFLP markers, and each successive backcross generation demonstrated a reduction of markers resulting in the 15-29% presence in the BC(2)F(2) Bulk population. Average fluorescence of each successive hybrid generation was analyzed, and homozygous canola lines and hybrid populations that contained individuals homozygous for GFP (BC(2)F(2) Bulk) demonstrated significantly higher fluorescence than hemizygous hybrid generations (F(1), BC(1)F(1) and BC(2)F(1)). These data demonstrate that the formation of homozygous individuals within hybrid populations increases the average level of transgene expression as generations progress. This phenomenon must be considered in the development of risk-management strategies. |
| doi_str_mv | 10.1007/s00122-003-1397-7 |
| format | Article |
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D ; MILLWOOD, R. J ; WEISSINGER, A. K ; WARWICK, S. I ; STEWART, C. N</creator><creatorcontrib>HALFHILL, M. D ; MILLWOOD, R. J ; WEISSINGER, A. K ; WARWICK, S. I ; STEWART, C. N</creatorcontrib><description>The level of transgene expression in crop x weed hybrids and the degree to which crop-specific genes are integrated into hybrid populations are important factors in assessing the potential ecological and agricultural risks of gene flow associated with genetic engineering. The average transgene zygosity and genetic structure of transgenic hybrid populations change with the progression of generations, and the green fluorescent protein (GFP) transgene is an ideal marker to quantify transgene expression in advancing populations. The homozygous T(1) single-locus insert GFP/ Bacillus thuringiensis (Bt) transgenic canola ( Brassica napus, cv Westar) with two copies of the transgene fluoresced twice as much as hemizygous individuals with only one copy of the transgene. These data indicate that the expression of the GFP gene was additive, and fluorescence could be used to determine zygosity status. Several hybrid generations (BC(1)F(1), BC(2)F(1)) were produced by backcrossing various GFP/Bt transgenic canola ( B. napus, cv Westar) and birdseed rape ( Brassica rapa) hybrid generations onto B. rapa. Intercrossed generations (BC(2)F(2) Bulk) were generated by crossing BC(2)F(1) individuals in the presence of a pollinating insect ( Musca domestica L.). The ploidy of plants in the BC(2)F(2) Bulk hybrid generation was identical to the weedy parental species, B. rapa. AFLP analysis was used to quantify the degree of B. napus introgression into multiple backcross hybrid generations with B. rapa. The F(1) hybrid generations contained 95-97% of the B. napus-specific AFLP markers, and each successive backcross generation demonstrated a reduction of markers resulting in the 15-29% presence in the BC(2)F(2) Bulk population. Average fluorescence of each successive hybrid generation was analyzed, and homozygous canola lines and hybrid populations that contained individuals homozygous for GFP (BC(2)F(2) Bulk) demonstrated significantly higher fluorescence than hemizygous hybrid generations (F(1), BC(1)F(1) and BC(2)F(1)). These data demonstrate that the formation of homozygous individuals within hybrid populations increases the average level of transgene expression as generations progress. This phenomenon must be considered in the development of risk-management strategies.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-003-1397-7</identifier><identifier>PMID: 13679991</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Agricultural research ; Agronomy. Soil science and plant productions ; Bacillus thuringiensis ; Biological and medical sciences ; Brassica napus ; Brassica rapa ; Classical genetics, quantitative genetics, hybrids ; Crops, Agricultural - genetics ; Crosses, Genetic ; Fluorescent proteins ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genetic aspects ; Genetic transformation ; Genetically modified crops ; Genetics and breeding of economic plants ; Genetics of eukaryotes. Biological and molecular evolution ; Green Fluorescent Proteins ; Hybridization, Vegetable ; Interspecific and intergeneric hybridization, introgressions ; introgression ; Luminescent Proteins - genetics ; Plant breeding: fundamental aspects and methodology ; Plants, Genetically Modified ; Pteridophyta, spermatophyta ; risk management ; Spectrometry, Fluorescence ; Transgenes ; Vegetals ; Weeds</subject><ispartof>Theoretical and applied genetics, 2003-11, Vol.107 (8), p.1533-1540</ispartof><rights>2004 INIST-CNRS</rights><rights>COPYRIGHT 2003 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15297070$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13679991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HALFHILL, M. D</creatorcontrib><creatorcontrib>MILLWOOD, R. J</creatorcontrib><creatorcontrib>WEISSINGER, A. K</creatorcontrib><creatorcontrib>WARWICK, S. I</creatorcontrib><creatorcontrib>STEWART, C. N</creatorcontrib><title>Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>The level of transgene expression in crop x weed hybrids and the degree to which crop-specific genes are integrated into hybrid populations are important factors in assessing the potential ecological and agricultural risks of gene flow associated with genetic engineering. The average transgene zygosity and genetic structure of transgenic hybrid populations change with the progression of generations, and the green fluorescent protein (GFP) transgene is an ideal marker to quantify transgene expression in advancing populations. The homozygous T(1) single-locus insert GFP/ Bacillus thuringiensis (Bt) transgenic canola ( Brassica napus, cv Westar) with two copies of the transgene fluoresced twice as much as hemizygous individuals with only one copy of the transgene. These data indicate that the expression of the GFP gene was additive, and fluorescence could be used to determine zygosity status. Several hybrid generations (BC(1)F(1), BC(2)F(1)) were produced by backcrossing various GFP/Bt transgenic canola ( B. napus, cv Westar) and birdseed rape ( Brassica rapa) hybrid generations onto B. rapa. Intercrossed generations (BC(2)F(2) Bulk) were generated by crossing BC(2)F(1) individuals in the presence of a pollinating insect ( Musca domestica L.). The ploidy of plants in the BC(2)F(2) Bulk hybrid generation was identical to the weedy parental species, B. rapa. AFLP analysis was used to quantify the degree of B. napus introgression into multiple backcross hybrid generations with B. rapa. The F(1) hybrid generations contained 95-97% of the B. napus-specific AFLP markers, and each successive backcross generation demonstrated a reduction of markers resulting in the 15-29% presence in the BC(2)F(2) Bulk population. Average fluorescence of each successive hybrid generation was analyzed, and homozygous canola lines and hybrid populations that contained individuals homozygous for GFP (BC(2)F(2) Bulk) demonstrated significantly higher fluorescence than hemizygous hybrid generations (F(1), BC(1)F(1) and BC(2)F(1)). These data demonstrate that the formation of homozygous individuals within hybrid populations increases the average level of transgene expression as generations progress. This phenomenon must be considered in the development of risk-management strategies.</description><subject>Agricultural research</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Bacillus thuringiensis</subject><subject>Biological and medical sciences</subject><subject>Brassica napus</subject><subject>Brassica rapa</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Crops, Agricultural - genetics</subject><subject>Crosses, Genetic</subject><subject>Fluorescent proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Genetic transformation</subject><subject>Genetically modified crops</subject><subject>Genetics and breeding of economic plants</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Green Fluorescent Proteins</subject><subject>Hybridization, Vegetable</subject><subject>Interspecific and intergeneric hybridization, introgressions</subject><subject>introgression</subject><subject>Luminescent Proteins - genetics</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plants, Genetically Modified</subject><subject>Pteridophyta, spermatophyta</subject><subject>risk management</subject><subject>Spectrometry, Fluorescence</subject><subject>Transgenes</subject><subject>Vegetals</subject><subject>Weeds</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7jj6A7xILgoeopWkk0yOw-LHwoLgx7lJd6rbSE-6TdI6-wv822aZGTx6Kqh66qGol5DnHN5wAPM2A3AhGIBkXFrDzAOy4Y0UTIhGPCQbgAaYMkpckSc5_wAAoUA-JldcamOt5RvyZ-99KOEX0pJczCNGpHhcEuYc5khd9PS-V0JPQyxpHi-TEOlhnUpYJqS1iZEN0zrXaY-x0CXNBStykdb1Ps0LPdLfiJ5-v-tSOJmTK1WXn5JHg5syPjvXLfn2_t3X64_s9tOHm-v9LRsliMI0KOHNYDl2aE2n0ethUKbjO993OyGHnjvnvDHODKi04F3HYWcHrVE2Fqzcklcnb73w54q5tIdQT54mF3Fec2u4NEIo_V-QW26tsvfG1ydwdBO2IfZzLHgso1tzbm--fG73WjXVKWtMW_LiLF27A_p2SeHg0l17yaMCL8-Ay72bhvq-PuR_nBLWgAH5F9Z0nTE</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>HALFHILL, M. 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Biological and molecular evolution</topic><topic>Green Fluorescent Proteins</topic><topic>Hybridization, Vegetable</topic><topic>Interspecific and intergeneric hybridization, introgressions</topic><topic>introgression</topic><topic>Luminescent Proteins - genetics</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plants, Genetically Modified</topic><topic>Pteridophyta, spermatophyta</topic><topic>risk management</topic><topic>Spectrometry, Fluorescence</topic><topic>Transgenes</topic><topic>Vegetals</topic><topic>Weeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HALFHILL, M. D</creatorcontrib><creatorcontrib>MILLWOOD, R. J</creatorcontrib><creatorcontrib>WEISSINGER, A. K</creatorcontrib><creatorcontrib>WARWICK, S. I</creatorcontrib><creatorcontrib>STEWART, C. 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N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>107</volume><issue>8</issue><spage>1533</spage><epage>1540</epage><pages>1533-1540</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>The level of transgene expression in crop x weed hybrids and the degree to which crop-specific genes are integrated into hybrid populations are important factors in assessing the potential ecological and agricultural risks of gene flow associated with genetic engineering. The average transgene zygosity and genetic structure of transgenic hybrid populations change with the progression of generations, and the green fluorescent protein (GFP) transgene is an ideal marker to quantify transgene expression in advancing populations. The homozygous T(1) single-locus insert GFP/ Bacillus thuringiensis (Bt) transgenic canola ( Brassica napus, cv Westar) with two copies of the transgene fluoresced twice as much as hemizygous individuals with only one copy of the transgene. These data indicate that the expression of the GFP gene was additive, and fluorescence could be used to determine zygosity status. Several hybrid generations (BC(1)F(1), BC(2)F(1)) were produced by backcrossing various GFP/Bt transgenic canola ( B. napus, cv Westar) and birdseed rape ( Brassica rapa) hybrid generations onto B. rapa. Intercrossed generations (BC(2)F(2) Bulk) were generated by crossing BC(2)F(1) individuals in the presence of a pollinating insect ( Musca domestica L.). The ploidy of plants in the BC(2)F(2) Bulk hybrid generation was identical to the weedy parental species, B. rapa. AFLP analysis was used to quantify the degree of B. napus introgression into multiple backcross hybrid generations with B. rapa. The F(1) hybrid generations contained 95-97% of the B. napus-specific AFLP markers, and each successive backcross generation demonstrated a reduction of markers resulting in the 15-29% presence in the BC(2)F(2) Bulk population. Average fluorescence of each successive hybrid generation was analyzed, and homozygous canola lines and hybrid populations that contained individuals homozygous for GFP (BC(2)F(2) Bulk) demonstrated significantly higher fluorescence than hemizygous hybrid generations (F(1), BC(1)F(1) and BC(2)F(1)). These data demonstrate that the formation of homozygous individuals within hybrid populations increases the average level of transgene expression as generations progress. This phenomenon must be considered in the development of risk-management strategies.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>13679991</pmid><doi>10.1007/s00122-003-1397-7</doi><tpages>8</tpages></addata></record> |
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| subjects | Agricultural research Agronomy. Soil science and plant productions Bacillus thuringiensis Biological and medical sciences Brassica napus Brassica rapa Classical genetics, quantitative genetics, hybrids Crops, Agricultural - genetics Crosses, Genetic Fluorescent proteins Fundamental and applied biological sciences. Psychology Gene expression Genetic aspects Genetic transformation Genetically modified crops Genetics and breeding of economic plants Genetics of eukaryotes. Biological and molecular evolution Green Fluorescent Proteins Hybridization, Vegetable Interspecific and intergeneric hybridization, introgressions introgression Luminescent Proteins - genetics Plant breeding: fundamental aspects and methodology Plants, Genetically Modified Pteridophyta, spermatophyta risk management Spectrometry, Fluorescence Transgenes Vegetals Weeds |
| title | Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop x weed hybrid generations |
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