Molecular cloning of Odf3 encoding a novel coiled-coil protein of sperm tail outer dense fibers
The outer dense fibers (ODF) are the main cytoskeletal structures of the sperm tail found in animals with internal fecundation. They consist of at least 14 polypeptides from which only a few are identified due to difficulties in isolation of the protein components. Here we report the isolation and m...
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| Veröffentlicht in: | Molecular reproduction and development 2002-01, Vol.61 (1), p.102-112 |
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| creator | Petersen, Christoph Aumüller, Gerhard Bahrami, Masoud Hoyer-Fender, Sigrid |
| description | The outer dense fibers (ODF) are the main cytoskeletal structures of the sperm tail found in animals with internal fecundation. They consist of at least 14 polypeptides from which only a few are identified due to difficulties in isolation of the protein components. Here we report the isolation and molecular characterization of Odf3, encoding a novel protein of rat sperm ODF. Odf3 is transcribed in testes and more specifically in spermatids but it is also expressed in epididymides and brain suggesting a possible involvement in building of the cellular cytoskeleton. Odf3 encodes a putative protein of ∼110 kDa. Secondary structure predictions indicated that ODF3 is a coiled‐coil protein. The identification of coiled‐coil proteins as constituents of outer dense fibers reveals a model for ODF formation. Mol. Reprod. Dev. 61: 102–112, 2002. © 2002 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/mrd.1136 |
| format | Article |
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They consist of at least 14 polypeptides from which only a few are identified due to difficulties in isolation of the protein components. Here we report the isolation and molecular characterization of Odf3, encoding a novel protein of rat sperm ODF. Odf3 is transcribed in testes and more specifically in spermatids but it is also expressed in epididymides and brain suggesting a possible involvement in building of the cellular cytoskeleton. Odf3 encodes a putative protein of ∼110 kDa. Secondary structure predictions indicated that ODF3 is a coiled‐coil protein. The identification of coiled‐coil proteins as constituents of outer dense fibers reveals a model for ODF formation. Mol. Reprod. Dev. 61: 102–112, 2002. © 2002 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.1136</identifier><identifier>PMID: 11774381</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Blotting, Western ; Cloning, Molecular ; coiled-coil proteins ; cytoskeletal proteins ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Genetic engineering ; Genetic technics ; Heat-Shock Proteins ; In Situ Hybridization ; Male ; Methods. Procedures. Technologies ; Microscopy, Electron ; Molecular cloning ; Molecular Sequence Data ; Odf3 gene ; outer dense fibers ; Polymerase Chain Reaction ; Proteins - genetics ; Rats ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; sperm tail ; Sperm Tail - metabolism ; Sperm Tail - ultrastructure ; spermatogenesis ; Transcription, Genetic</subject><ispartof>Molecular reproduction and development, 2002-01, Vol.61 (1), p.102-112</ispartof><rights>Copyright © 2002 Wiley‐Liss, Inc.</rights><rights>2002 INIST-CNRS</rights><rights>Copyright 2002 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4826-79bdbf8632caedb8720dcd5a77ba2cee2857de1f642306a6886335c2074d583d3</citedby><cites>FETCH-LOGICAL-c4826-79bdbf8632caedb8720dcd5a77ba2cee2857de1f642306a6886335c2074d583d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.1136$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.1136$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13386033$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11774381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petersen, Christoph</creatorcontrib><creatorcontrib>Aumüller, Gerhard</creatorcontrib><creatorcontrib>Bahrami, Masoud</creatorcontrib><creatorcontrib>Hoyer-Fender, Sigrid</creatorcontrib><title>Molecular cloning of Odf3 encoding a novel coiled-coil protein of sperm tail outer dense fibers</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>The outer dense fibers (ODF) are the main cytoskeletal structures of the sperm tail found in animals with internal fecundation. They consist of at least 14 polypeptides from which only a few are identified due to difficulties in isolation of the protein components. Here we report the isolation and molecular characterization of Odf3, encoding a novel protein of rat sperm ODF. Odf3 is transcribed in testes and more specifically in spermatids but it is also expressed in epididymides and brain suggesting a possible involvement in building of the cellular cytoskeleton. Odf3 encodes a putative protein of ∼110 kDa. Secondary structure predictions indicated that ODF3 is a coiled‐coil protein. The identification of coiled‐coil proteins as constituents of outer dense fibers reveals a model for ODF formation. Mol. Reprod. Dev. 61: 102–112, 2002. © 2002 Wiley‐Liss, Inc.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>coiled-coil proteins</subject><subject>cytoskeletal proteins</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Heat-Shock Proteins</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Methods. Procedures. Technologies</subject><subject>Microscopy, Electron</subject><subject>Molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>Odf3 gene</subject><subject>outer dense fibers</subject><subject>Polymerase Chain Reaction</subject><subject>Proteins - genetics</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>sperm tail</subject><subject>Sperm Tail - metabolism</subject><subject>Sperm Tail - ultrastructure</subject><subject>spermatogenesis</subject><subject>Transcription, Genetic</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MtO3TAQBmCrKiq3Sn2CyptWbAK-JLazrE6BXg4gIXrZWY49QaFOfLATWt6-jk5UVhWrGY0-zYx-hN5QckwJYSd9dMeUcvEC7VFSq4LJuno59yUpyor93EX7Kd0RQupakVdol1IpS67oHtIXwYOdvInY-jB0wy0OLb5yLccw2ODmgcFDeACPbeg8uGIueBPDCN0w47SB2OPR5GmYRojYwZAAt10DMR2indb4BK-XeoC-nZ3erD4V66vzz6sP68KWiolC1o1rWiU4swZcoyQjzrrKSNkYZgGYqqQD2oqScSKMUJnyyjIiS1cp7vgBer_dmx-7nyCNuu-SBe_NAGFKWuZ0hBTiWUjzO2VF6wyPttDGkFKEVm9i15v4qCnRc-o6p67n1DN9u-ycmh7cE1xizuDdAkyyxrfRDLZLT45zJQjn2RVb9zsn_fjfg_ri-uNyePFdGuHPP2_iLy0kl5X-cXmuBWNfv6_Ktf7C_wKXsqbq</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Petersen, Christoph</creator><creator>Aumüller, Gerhard</creator><creator>Bahrami, Masoud</creator><creator>Hoyer-Fender, Sigrid</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200201</creationdate><title>Molecular cloning of Odf3 encoding a novel coiled-coil protein of sperm tail outer dense fibers</title><author>Petersen, Christoph ; Aumüller, Gerhard ; Bahrami, Masoud ; Hoyer-Fender, Sigrid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4826-79bdbf8632caedb8720dcd5a77ba2cee2857de1f642306a6886335c2074d583d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>coiled-coil proteins</topic><topic>cytoskeletal proteins</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Heat-Shock Proteins</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Methods. Procedures. Technologies</topic><topic>Microscopy, Electron</topic><topic>Molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>Odf3 gene</topic><topic>outer dense fibers</topic><topic>Polymerase Chain Reaction</topic><topic>Proteins - genetics</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>sperm tail</topic><topic>Sperm Tail - metabolism</topic><topic>Sperm Tail - ultrastructure</topic><topic>spermatogenesis</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petersen, Christoph</creatorcontrib><creatorcontrib>Aumüller, Gerhard</creatorcontrib><creatorcontrib>Bahrami, Masoud</creatorcontrib><creatorcontrib>Hoyer-Fender, Sigrid</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petersen, Christoph</au><au>Aumüller, Gerhard</au><au>Bahrami, Masoud</au><au>Hoyer-Fender, Sigrid</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of Odf3 encoding a novel coiled-coil protein of sperm tail outer dense fibers</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2002-01</date><risdate>2002</risdate><volume>61</volume><issue>1</issue><spage>102</spage><epage>112</epage><pages>102-112</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>The outer dense fibers (ODF) are the main cytoskeletal structures of the sperm tail found in animals with internal fecundation. They consist of at least 14 polypeptides from which only a few are identified due to difficulties in isolation of the protein components. Here we report the isolation and molecular characterization of Odf3, encoding a novel protein of rat sperm ODF. Odf3 is transcribed in testes and more specifically in spermatids but it is also expressed in epididymides and brain suggesting a possible involvement in building of the cellular cytoskeleton. Odf3 encodes a putative protein of ∼110 kDa. Secondary structure predictions indicated that ODF3 is a coiled‐coil protein. 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| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Biotechnology Blotting, Western Cloning, Molecular coiled-coil proteins cytoskeletal proteins DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Developmental Genetic engineering Genetic technics Heat-Shock Proteins In Situ Hybridization Male Methods. Procedures. Technologies Microscopy, Electron Molecular cloning Molecular Sequence Data Odf3 gene outer dense fibers Polymerase Chain Reaction Proteins - genetics Rats RNA, Messenger - genetics RNA, Messenger - metabolism sperm tail Sperm Tail - metabolism Sperm Tail - ultrastructure spermatogenesis Transcription, Genetic |
| title | Molecular cloning of Odf3 encoding a novel coiled-coil protein of sperm tail outer dense fibers |
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