Regulation of the Rat SREBP-1c Promoter in Primary Rat Hepatocytes

We have cloned 5 kb of genomic DNA encompassing 1.72 kb of 5′-regulatory sequence and exons 1-c and 2 of the rat SREBP-1c gene. A 1.5-kb segment upstream from the transcription start site was ligated ahead of the luciferase reporter gene and tested for promoter activity by transient transfection ass...

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Veröffentlicht in:	Biochemical and biophysical research communications 2002-01, Vol.290 (1), p.256-262
Hauptverfasser:	Deng, Xiong, Cagen, Lauren M., Wilcox, Henry G., Park, Edwards A., Raghow, Rajendra, Elam, Marshall B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Bucladesine - metabolism CCAAT-Enhancer-Binding Proteins - biosynthesis CCAAT-Enhancer-Binding Proteins - genetics Cells, Cultured Cloning, Molecular Cyclic AMP - metabolism DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics Eicosapentaenoic Acid - metabolism Enzyme Inhibitors - pharmacology Exons gamma-Linolenic Acid - metabolism Gene Expression Regulation Genes, Reporter Glucose - pharmacology Hepatocytes - metabolism Insulin - metabolism Liver - metabolism Luciferases - metabolism Male Molecular Sequence Data Oleic Acid - metabolism Palmitic Acid - metabolism Plasmids - metabolism Promoter Regions, Genetic Rats Rats, Sprague-Dawley RNA - metabolism RNA, Messenger - metabolism SREBP-1c protein Sterol Regulatory Element Binding Protein 1 sterol regulatory element-binding protein 1c Time Factors Transcription Factors Transcription, Genetic Transfection
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