HIV-1 transmission and cytokine-induced expression of DC-SIGN in human monocyte-derived macrophages
Dendritic cell‐specific intercellular adhesion molecule‐3‐grabbing nonintegrin (DC‐SIGN) has been described as an attachment molecule for human immunodeficiency virus type 1 (HIV‐1) with the potential to mediate its transmission. We examined DC‐SIGN expression in monocyte‐derived macrophages (MDM) a...
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| Veröffentlicht in: | Journal of leukocyte biology 2003-11, Vol.74 (5), p.757-763 |
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| container_title | Journal of leukocyte biology |
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| creator | Chehimi, Jihed Luo, Qi Azzoni, Livio Shawver, Linda Ngoubilly, Noel June, Ray Jerandi, Ghassen Farabaugh, Matthew Montaner, Luis J. |
| description | Dendritic cell‐specific intercellular adhesion molecule‐3‐grabbing nonintegrin (DC‐SIGN) has been described as an attachment molecule for human immunodeficiency virus type 1 (HIV‐1) with the potential to mediate its transmission. We examined DC‐SIGN expression in monocyte‐derived macrophages (MDM) and its role in viral transmission when MDM were exposed to interleukin (IL)‐13, IL‐4, or interferon‐γ (IFN‐γ). We show that IL‐13 and IL‐4 increase transcripts, total protein, and cell‐surface expression of DC‐SIGN in all MDM tested, IFN‐γ results ranged from no change to up‐regulation of surface expression, and message and total protein were, respectively, induced in all and 86% of donors tested. Transmission experiments of HIV‐1 X4 between cytokine‐treated MDM to Sup‐T1 cells showed no association between total transmission and DC‐SIGN up‐regulation. IL‐4 but not IL‐13 resulted in a less than twofold increase in MDM viral transmission to CD4+ T cells in spite of a fourfold up‐regulation in DC‐SIGN expression by either cytokine. In contrast, IFN‐γ treatment induced a decrease in total transmission by at least two‐thirds, despite its induction of DC‐SIGN. Soluble mannan resulted in a greater inhibition of viral transmission to CD4+ T cells than neutralizing anti‐DC‐SIGN monoclonal antibody (67–75% vs. 39–48%), supporting the role of mannose‐binding receptors in viral transmission. Taken together, results show that DC‐SIGN regulation in MDM does not singly predict the transmission potential of this cell type. |
| doi_str_mv | 10.1189/jlb.0503231 |
| format | Article |
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We examined DC‐SIGN expression in monocyte‐derived macrophages (MDM) and its role in viral transmission when MDM were exposed to interleukin (IL)‐13, IL‐4, or interferon‐γ (IFN‐γ). We show that IL‐13 and IL‐4 increase transcripts, total protein, and cell‐surface expression of DC‐SIGN in all MDM tested, IFN‐γ results ranged from no change to up‐regulation of surface expression, and message and total protein were, respectively, induced in all and 86% of donors tested. Transmission experiments of HIV‐1 X4 between cytokine‐treated MDM to Sup‐T1 cells showed no association between total transmission and DC‐SIGN up‐regulation. IL‐4 but not IL‐13 resulted in a less than twofold increase in MDM viral transmission to CD4+ T cells in spite of a fourfold up‐regulation in DC‐SIGN expression by either cytokine. In contrast, IFN‐γ treatment induced a decrease in total transmission by at least two‐thirds, despite its induction of DC‐SIGN. Soluble mannan resulted in a greater inhibition of viral transmission to CD4+ T cells than neutralizing anti‐DC‐SIGN monoclonal antibody (67–75% vs. 39–48%), supporting the role of mannose‐binding receptors in viral transmission. Taken together, results show that DC‐SIGN regulation in MDM does not singly predict the transmission potential of this cell type.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1189/jlb.0503231</identifier><identifier>PMID: 12960240</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>Acquired Immunodeficiency Syndrome - immunology ; Acquired Immunodeficiency Syndrome - transmission ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - virology ; Cell Adhesion Molecules - drug effects ; Cell Adhesion Molecules - genetics ; Cell Line ; Cells, Cultured ; Cytokines - immunology ; DC-SIGN protein ; HIV-1 ; Human immunodeficiency virus 1 ; Humans ; IFN‐γ ; IL‐13 ; IL‐4 ; Interferon-gamma - pharmacology ; Interleukin-13 - pharmacology ; Interleukin-4 - pharmacology ; Lectins, C-Type - drug effects ; Lectins, C-Type - genetics ; Macrophages - immunology ; Macrophages - virology ; Monocytes - immunology ; Monocytes - virology ; Receptors, Cell Surface - drug effects ; Receptors, Cell Surface - genetics ; Up-Regulation</subject><ispartof>Journal of leukocyte biology, 2003-11, Vol.74 (5), p.757-763</ispartof><rights>2003 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3877-b15f24110ddce6251ca3724a242c3e74512f6823a889b0c37398ef014764c2823</citedby><cites>FETCH-LOGICAL-c3877-b15f24110ddce6251ca3724a242c3e74512f6823a889b0c37398ef014764c2823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1189%2Fjlb.0503231$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1189%2Fjlb.0503231$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12960240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chehimi, Jihed</creatorcontrib><creatorcontrib>Luo, Qi</creatorcontrib><creatorcontrib>Azzoni, Livio</creatorcontrib><creatorcontrib>Shawver, Linda</creatorcontrib><creatorcontrib>Ngoubilly, Noel</creatorcontrib><creatorcontrib>June, Ray</creatorcontrib><creatorcontrib>Jerandi, Ghassen</creatorcontrib><creatorcontrib>Farabaugh, Matthew</creatorcontrib><creatorcontrib>Montaner, Luis J.</creatorcontrib><title>HIV-1 transmission and cytokine-induced expression of DC-SIGN in human monocyte-derived macrophages</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>Dendritic cell‐specific intercellular adhesion molecule‐3‐grabbing nonintegrin (DC‐SIGN) has been described as an attachment molecule for human immunodeficiency virus type 1 (HIV‐1) with the potential to mediate its transmission. We examined DC‐SIGN expression in monocyte‐derived macrophages (MDM) and its role in viral transmission when MDM were exposed to interleukin (IL)‐13, IL‐4, or interferon‐γ (IFN‐γ). We show that IL‐13 and IL‐4 increase transcripts, total protein, and cell‐surface expression of DC‐SIGN in all MDM tested, IFN‐γ results ranged from no change to up‐regulation of surface expression, and message and total protein were, respectively, induced in all and 86% of donors tested. Transmission experiments of HIV‐1 X4 between cytokine‐treated MDM to Sup‐T1 cells showed no association between total transmission and DC‐SIGN up‐regulation. IL‐4 but not IL‐13 resulted in a less than twofold increase in MDM viral transmission to CD4+ T cells in spite of a fourfold up‐regulation in DC‐SIGN expression by either cytokine. In contrast, IFN‐γ treatment induced a decrease in total transmission by at least two‐thirds, despite its induction of DC‐SIGN. Soluble mannan resulted in a greater inhibition of viral transmission to CD4+ T cells than neutralizing anti‐DC‐SIGN monoclonal antibody (67–75% vs. 39–48%), supporting the role of mannose‐binding receptors in viral transmission. Taken together, results show that DC‐SIGN regulation in MDM does not singly predict the transmission potential of this cell type.</description><subject>Acquired Immunodeficiency Syndrome - immunology</subject><subject>Acquired Immunodeficiency Syndrome - transmission</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Cell Adhesion Molecules - drug effects</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Cytokines - immunology</subject><subject>DC-SIGN protein</subject><subject>HIV-1</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>IFN‐γ</subject><subject>IL‐13</subject><subject>IL‐4</subject><subject>Interferon-gamma - pharmacology</subject><subject>Interleukin-13 - pharmacology</subject><subject>Interleukin-4 - pharmacology</subject><subject>Lectins, C-Type - drug effects</subject><subject>Lectins, C-Type - genetics</subject><subject>Macrophages - immunology</subject><subject>Macrophages - virology</subject><subject>Monocytes - immunology</subject><subject>Monocytes - virology</subject><subject>Receptors, Cell Surface - drug effects</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Up-Regulation</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0LFv1DAUBnALUdFry8SOssBSufjZTuyMcEB71QmGAqvlc156LolztS-E--9xlZPYYPLwft-z3kfIK2BXALp-99BtrljJBBfwjCygFpqKSonnZMGUBFpKxk7JWUoPjGVUsRfkFHhdMS7Zgrib1Q8KxT7akHqfkh9CYUNTuMN--OkDUh-a0WFT4O9dxHk-tMXHJb1bXX8pfCi2Y29D0Q9hyBmkDUb_K_veujjstvYe0wU5aW2X8OXxPSffP3_6tryh66_Xq-X7NXVCK0U3ULZcArCmcVjxEpwVikvLJXcClSyBt5Xmwmpdb5gTStQaWwZSVdLxPDgnb-e9uzg8jpj2Jl_ksOtswGFMRoGohNT6vxBqqHUJkOHlDPMtKUVszS763saDAWaeyje5fHMsP-vXx7Xjpsfmrz22nQGbweQ7PPxrl7ldf2CqVDnyZo5s_f128hFN6m3X5R-4maZJSVOaJ_cH0PWZ8g</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Chehimi, Jihed</creator><creator>Luo, Qi</creator><creator>Azzoni, Livio</creator><creator>Shawver, Linda</creator><creator>Ngoubilly, Noel</creator><creator>June, Ray</creator><creator>Jerandi, Ghassen</creator><creator>Farabaugh, Matthew</creator><creator>Montaner, Luis J.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>HIV-1 transmission and cytokine-induced expression of DC-SIGN in human monocyte-derived macrophages</title><author>Chehimi, Jihed ; 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We examined DC‐SIGN expression in monocyte‐derived macrophages (MDM) and its role in viral transmission when MDM were exposed to interleukin (IL)‐13, IL‐4, or interferon‐γ (IFN‐γ). We show that IL‐13 and IL‐4 increase transcripts, total protein, and cell‐surface expression of DC‐SIGN in all MDM tested, IFN‐γ results ranged from no change to up‐regulation of surface expression, and message and total protein were, respectively, induced in all and 86% of donors tested. Transmission experiments of HIV‐1 X4 between cytokine‐treated MDM to Sup‐T1 cells showed no association between total transmission and DC‐SIGN up‐regulation. IL‐4 but not IL‐13 resulted in a less than twofold increase in MDM viral transmission to CD4+ T cells in spite of a fourfold up‐regulation in DC‐SIGN expression by either cytokine. In contrast, IFN‐γ treatment induced a decrease in total transmission by at least two‐thirds, despite its induction of DC‐SIGN. Soluble mannan resulted in a greater inhibition of viral transmission to CD4+ T cells than neutralizing anti‐DC‐SIGN monoclonal antibody (67–75% vs. 39–48%), supporting the role of mannose‐binding receptors in viral transmission. Taken together, results show that DC‐SIGN regulation in MDM does not singly predict the transmission potential of this cell type.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>12960240</pmid><doi>10.1189/jlb.0503231</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of leukocyte biology, 2003-11, Vol.74 (5), p.757-763 |
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| source | Wiley-Blackwell Journals; MEDLINE; Oxford Academic Journals (OUP); EZB Electronic Journals Library |
| subjects | Acquired Immunodeficiency Syndrome - immunology Acquired Immunodeficiency Syndrome - transmission CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - virology Cell Adhesion Molecules - drug effects Cell Adhesion Molecules - genetics Cell Line Cells, Cultured Cytokines - immunology DC-SIGN protein HIV-1 Human immunodeficiency virus 1 Humans IFN‐γ IL‐13 IL‐4 Interferon-gamma - pharmacology Interleukin-13 - pharmacology Interleukin-4 - pharmacology Lectins, C-Type - drug effects Lectins, C-Type - genetics Macrophages - immunology Macrophages - virology Monocytes - immunology Monocytes - virology Receptors, Cell Surface - drug effects Receptors, Cell Surface - genetics Up-Regulation |
| title | HIV-1 transmission and cytokine-induced expression of DC-SIGN in human monocyte-derived macrophages |
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