A massively parallel PicoTiterPlate™ based platform for discrete picoliter-scale polymerase chain reactions

We demonstrate successful, simultaneous polymerase chain reaction (PCR) amplification of up to 300 000 discrete reactions in a novel platform, the PicoTiterPlate™. In addition to elevated throughput, the PicoTiterPlate™ based amplifications (PTPCR) can be performed in extremely small volumes: indivi...

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Veröffentlicht in:	Electrophoresis 2003-11, Vol.24 (21), p.3769-3777
Hauptverfasser:	Leamon, John H., Lee, William L., Tartaro, Karrie R., Lanza, Janna R., Sarkis, Gary J., deWinter, Alex D., Berka, Jan, Lohman, Kenton L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Base Sequence DNA - chemistry DNA - genetics DNA Primers Fiber Optic Technology Massively parallel Microscopy, Electron, Scanning Miniaturization - instrumentation Miniaturization - methods Nucleic Acid Hybridization - methods Picoliter Polymerase chain reaction Polymerase Chain Reaction - methods Sensitivity and Specificity
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