Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection
To investigate the clonality of ectopic gland cells in peritoneal endometriosis. Prospective study. University hospital. Seventeen women with surgically diagnosed endometriosis. Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery. Clonality analysis us...
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| Veröffentlicht in: | Fertility and sterility 2003-11, Vol.80 (5), p.1144-1150 |
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| container_title | Fertility and sterility |
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| creator | Nabeshima, Hiroshi Murakami, Takashi Yoshinaga, Kosuke Sato, Kazuyo Terada, Yukihiro Okamura, Kunihiro |
| description | To investigate the clonality of ectopic gland cells in peritoneal endometriosis.
Prospective study.
University hospital.
Seventeen women with surgically diagnosed endometriosis.
Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
Clonality analysis used the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay after digestion of the DNA with methylation-sensitive endonuclease.
Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis. |
| doi_str_mv | 10.1016/S0015-0282(03)01181-6 |
| format | Article |
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Prospective study.
University hospital.
Seventeen women with surgically diagnosed endometriosis.
Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
Clonality analysis used the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay after digestion of the DNA with methylation-sensitive endonuclease.
Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/S0015-0282(03)01181-6</identifier><identifier>PMID: 14607565</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>androgen receptor gene polymorphism ; Biological and medical sciences ; clonality ; Clone Cells - pathology ; DNA Methylation ; Endometriosis ; Endometriosis - pathology ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Heterozygote ; Homozygote ; Humans ; Hysteroscopy ; Laser Therapy ; Medical sciences ; Microdissection ; Non tumoral diseases ; Peritoneal Diseases - pathology ; Phosphoglycerate Kinase - genetics ; phosphoglycerate kinase gene polymorphism ; Polymorphism, Genetic ; Prospective Studies</subject><ispartof>Fertility and sterility, 2003-11, Vol.80 (5), p.1144-1150</ispartof><rights>2003 American Society for Reproductive Medicine</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-dcf475306942e7857f26c2ed46a46dbb565e72cad316c9060d45f445672262a63</citedby><cites>FETCH-LOGICAL-c504t-dcf475306942e7857f26c2ed46a46dbb565e72cad316c9060d45f445672262a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0015-0282(03)01181-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,778,782,787,788,3539,23913,23914,25123,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15265606$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14607565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nabeshima, Hiroshi</creatorcontrib><creatorcontrib>Murakami, Takashi</creatorcontrib><creatorcontrib>Yoshinaga, Kosuke</creatorcontrib><creatorcontrib>Sato, Kazuyo</creatorcontrib><creatorcontrib>Terada, Yukihiro</creatorcontrib><creatorcontrib>Okamura, Kunihiro</creatorcontrib><title>Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>To investigate the clonality of ectopic gland cells in peritoneal endometriosis.
Prospective study.
University hospital.
Seventeen women with surgically diagnosed endometriosis.
Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
Clonality analysis used the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay after digestion of the DNA with methylation-sensitive endonuclease.
Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.</description><subject>androgen receptor gene polymorphism</subject><subject>Biological and medical sciences</subject><subject>clonality</subject><subject>Clone Cells - pathology</subject><subject>DNA Methylation</subject><subject>Endometriosis</subject><subject>Endometriosis - pathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Heterozygote</subject><subject>Homozygote</subject><subject>Humans</subject><subject>Hysteroscopy</subject><subject>Laser Therapy</subject><subject>Medical sciences</subject><subject>Microdissection</subject><subject>Non tumoral diseases</subject><subject>Peritoneal Diseases - pathology</subject><subject>Phosphoglycerate Kinase - genetics</subject><subject>phosphoglycerate kinase gene polymorphism</subject><subject>Polymorphism, Genetic</subject><subject>Prospective Studies</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhJ4B8oYJDytixJ7snVFV8SZV6AM6W154UoyRePFmk_fd1uit65DTS6HlnXj1CvFZwqUDhh-8Ayjag1_odtO9BqbVq8IlYKWuxsWjbp2L1DzkTL5h_AwCqTj8XZ8ogdBVaiXA1-eHAiWXu5fyLZBhy3aT5sCwozHmXgrwb_BRZpknuqKQ5T-QHSVPMI80l5SW-5zTdycEzFTmmUHJMzDWf8vRSPOv9wPTqNM_Fz8-fflx_bW5uv3y7vrppggUzNzH0prMt4MZo6ta26zUGTdGgNxi329qXOh18bBWGDSBEY3tjLHZao_bYnouL491dyX_2xLMbEwcaannKe3adaq3doKmgPYK1JnOh3u1KGn05OAVusese7LpFnYPWPdh1y4M3pwf77UjxMXXSWYG3J8Bz8ENf_BQSP3JWo0VYDn08clR1_E1UHIdEU6CYSnXmYk7_qXIPoAyXLQ</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Nabeshima, Hiroshi</creator><creator>Murakami, Takashi</creator><creator>Yoshinaga, Kosuke</creator><creator>Sato, Kazuyo</creator><creator>Terada, Yukihiro</creator><creator>Okamura, Kunihiro</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection</title><author>Nabeshima, Hiroshi ; Murakami, Takashi ; Yoshinaga, Kosuke ; Sato, Kazuyo ; Terada, Yukihiro ; Okamura, Kunihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-dcf475306942e7857f26c2ed46a46dbb565e72cad316c9060d45f445672262a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>androgen receptor gene polymorphism</topic><topic>Biological and medical sciences</topic><topic>clonality</topic><topic>Clone Cells - pathology</topic><topic>DNA Methylation</topic><topic>Endometriosis</topic><topic>Endometriosis - pathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Heterozygote</topic><topic>Homozygote</topic><topic>Humans</topic><topic>Hysteroscopy</topic><topic>Laser Therapy</topic><topic>Medical sciences</topic><topic>Microdissection</topic><topic>Non tumoral diseases</topic><topic>Peritoneal Diseases - pathology</topic><topic>Phosphoglycerate Kinase - genetics</topic><topic>phosphoglycerate kinase gene polymorphism</topic><topic>Polymorphism, Genetic</topic><topic>Prospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nabeshima, Hiroshi</creatorcontrib><creatorcontrib>Murakami, Takashi</creatorcontrib><creatorcontrib>Yoshinaga, Kosuke</creatorcontrib><creatorcontrib>Sato, Kazuyo</creatorcontrib><creatorcontrib>Terada, Yukihiro</creatorcontrib><creatorcontrib>Okamura, Kunihiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nabeshima, Hiroshi</au><au>Murakami, Takashi</au><au>Yoshinaga, Kosuke</au><au>Sato, Kazuyo</au><au>Terada, Yukihiro</au><au>Okamura, Kunihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>80</volume><issue>5</issue><spage>1144</spage><epage>1150</epage><pages>1144-1150</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>To investigate the clonality of ectopic gland cells in peritoneal endometriosis.
Prospective study.
University hospital.
Seventeen women with surgically diagnosed endometriosis.
Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
Clonality analysis used the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay after digestion of the DNA with methylation-sensitive endonuclease.
Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>14607565</pmid><doi>10.1016/S0015-0282(03)01181-6</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | androgen receptor gene polymorphism Biological and medical sciences clonality Clone Cells - pathology DNA Methylation Endometriosis Endometriosis - pathology Female Female genital diseases Gynecology. Andrology. Obstetrics Heterozygote Homozygote Humans Hysteroscopy Laser Therapy Medical sciences Microdissection Non tumoral diseases Peritoneal Diseases - pathology Phosphoglycerate Kinase - genetics phosphoglycerate kinase gene polymorphism Polymorphism, Genetic Prospective Studies |
| title | Analysis of the clonality of ectopic glands in peritoneal endometriosis using laser microdissection |
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