IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites
The requirement for IFN-γ and/or TNF-α as co-stimulants with Babesia bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of ex...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2002-01, Vol.84 (3), p.237-251 |
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| creator | Goff, W.L Johnson, W.C Parish, S.M Barrington, G.M Elsasser, T.H Davis, W.C Valdez, R.A |
| description | The requirement for IFN-γ and/or TNF-α as co-stimulants with
Babesia
bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified
B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN-γ and TNF-α unless the monocytes taken ex vivo were producing TNF-α endogenously. Under the latter condition, the NO production resulting from merozoite stimulation remained IFN-γ-dependent. There was no evidence for endogenous synthesis of TNF-α in monocyte-derived macrophages (MDM), and merozoites alone were incapable of inducing TNF-α mRNA in MDM. However, while merozoites plus IFN-γ induced TNF-α mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes. |
| doi_str_mv | 10.1016/S0165-2427(01)00413-5 |
| format | Article |
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Babesia
bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified
B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN-γ and TNF-α unless the monocytes taken ex vivo were producing TNF-α endogenously. Under the latter condition, the NO production resulting from merozoite stimulation remained IFN-γ-dependent. There was no evidence for endogenous synthesis of TNF-α in monocyte-derived macrophages (MDM), and merozoites alone were incapable of inducing TNF-α mRNA in MDM. However, while merozoites plus IFN-γ induced TNF-α mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/S0165-2427(01)00413-5</identifier><identifier>PMID: 11777537</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Babesia bovis ; Babesia bovis - growth & development ; Babesia bovis - immunology ; Babesia bovis - pathogenicity ; Bovine ; Cattle ; Cell Differentiation ; Cytokines ; g-Interferon ; Gene Expression - drug effects ; In Vitro Techniques ; iNOS/nitric oxide ; Interferon-gamma - genetics ; Interferon-gamma - metabolism ; Interferon-gamma - pharmacology ; Interleukin-10 - pharmacology ; Interleukin-4 - pharmacology ; Macrophages - drug effects ; Macrophages - immunology ; Macrophages - metabolism ; Macrophages - parasitology ; Monocyte/macrophage ; Monocytes - drug effects ; Monocytes - immunology ; Monocytes - metabolism ; Monocytes - parasitology ; nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - genetics ; Nitric Oxide Synthase - metabolism ; Nitric Oxide Synthase Type II ; Phagocytes - drug effects ; Phagocytes - immunology ; Phagocytes - metabolism ; Phagocytes - parasitology ; Recombinant Proteins ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Veterinary immunology and immunopathology, 2002-01, Vol.84 (3), p.237-251</ispartof><rights>2002 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-cd4bf331d06154df5486093366f4480cdb341ff469e464e2cc5c4c21c1d07a983</citedby><cites>FETCH-LOGICAL-c392t-cd4bf331d06154df5486093366f4480cdb341ff469e464e2cc5c4c21c1d07a983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242701004135$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11777537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goff, W.L</creatorcontrib><creatorcontrib>Johnson, W.C</creatorcontrib><creatorcontrib>Parish, S.M</creatorcontrib><creatorcontrib>Barrington, G.M</creatorcontrib><creatorcontrib>Elsasser, T.H</creatorcontrib><creatorcontrib>Davis, W.C</creatorcontrib><creatorcontrib>Valdez, R.A</creatorcontrib><title>IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>The requirement for IFN-γ and/or TNF-α as co-stimulants with
Babesia
bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified
B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN-γ and TNF-α unless the monocytes taken ex vivo were producing TNF-α endogenously. Under the latter condition, the NO production resulting from merozoite stimulation remained IFN-γ-dependent. There was no evidence for endogenous synthesis of TNF-α in monocyte-derived macrophages (MDM), and merozoites alone were incapable of inducing TNF-α mRNA in MDM. However, while merozoites plus IFN-γ induced TNF-α mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes.</description><subject>Animals</subject><subject>Babesia bovis</subject><subject>Babesia bovis - growth & development</subject><subject>Babesia bovis - immunology</subject><subject>Babesia bovis - pathogenicity</subject><subject>Bovine</subject><subject>Cattle</subject><subject>Cell Differentiation</subject><subject>Cytokines</subject><subject>g-Interferon</subject><subject>Gene Expression - drug effects</subject><subject>In Vitro Techniques</subject><subject>iNOS/nitric oxide</subject><subject>Interferon-gamma - genetics</subject><subject>Interferon-gamma - metabolism</subject><subject>Interferon-gamma - pharmacology</subject><subject>Interleukin-10 - pharmacology</subject><subject>Interleukin-4 - pharmacology</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - parasitology</subject><subject>Monocyte/macrophage</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - immunology</subject><subject>Monocytes - metabolism</subject><subject>Monocytes - parasitology</subject><subject>nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - genetics</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>Nitric Oxide Synthase Type II</subject><subject>Phagocytes - drug effects</subject><subject>Phagocytes - immunology</subject><subject>Phagocytes - metabolism</subject><subject>Phagocytes - parasitology</subject><subject>Recombinant Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi0EokPhEUBeIVgYfGM7PytUKgZGGpUFZW0l9jU1mtjBTqqWJ-E1EO_RZyKTGcGyG9uSz_dd2YeQ58DfAIfy7Zd5UayQRfWKw2vOJQimHpAV1JVghRLyIVn9Q07Ik5y_c85VU9ePyQlAVVVKVCvya7NlkrbB0vkAnPpw5Ts_-hhodHSzvmB3f9hyf3mxZne_mcUBg8Uw0uDH5A2NN94iHVK0k1lyLsWedvHaB6R9DDFMZodtosNV-y2a2xEzxZshZrR0jPR922H27RLItMcUf0Y_M0_JI9fuMj477qfk6_rD5fkntv38cXN-tmVGNMXIjJWdEwIsL0FJ65SsS94IUZZOypob2wkJzsmyQVlKLIxRRpoCzJyo2qYWp-TloXd-wY8J86h7nw3udm3AOGVdgZg7Ae4FoS5KEGLfqA6gSTHnhE4PyfdtutXA9d6dXtzpvRjNQS_utJpzL44Dpq5H-z91lDUD7w4Azv9x7THpbDwGg9YnNKO20d8z4i-CYqp4</recordid><startdate>20020115</startdate><enddate>20020115</enddate><creator>Goff, W.L</creator><creator>Johnson, W.C</creator><creator>Parish, S.M</creator><creator>Barrington, G.M</creator><creator>Elsasser, T.H</creator><creator>Davis, W.C</creator><creator>Valdez, R.A</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20020115</creationdate><title>IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites</title><author>Goff, W.L ; Johnson, W.C ; Parish, S.M ; Barrington, G.M ; Elsasser, T.H ; Davis, W.C ; Valdez, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-cd4bf331d06154df5486093366f4480cdb341ff469e464e2cc5c4c21c1d07a983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Babesia bovis</topic><topic>Babesia bovis - growth & development</topic><topic>Babesia bovis - immunology</topic><topic>Babesia bovis - pathogenicity</topic><topic>Bovine</topic><topic>Cattle</topic><topic>Cell Differentiation</topic><topic>Cytokines</topic><topic>g-Interferon</topic><topic>Gene Expression - drug effects</topic><topic>In Vitro Techniques</topic><topic>iNOS/nitric oxide</topic><topic>Interferon-gamma - genetics</topic><topic>Interferon-gamma - metabolism</topic><topic>Interferon-gamma - pharmacology</topic><topic>Interleukin-10 - pharmacology</topic><topic>Interleukin-4 - pharmacology</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - immunology</topic><topic>Macrophages - metabolism</topic><topic>Macrophages - parasitology</topic><topic>Monocyte/macrophage</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - immunology</topic><topic>Monocytes - metabolism</topic><topic>Monocytes - parasitology</topic><topic>nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - genetics</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>Nitric Oxide Synthase Type II</topic><topic>Phagocytes - drug effects</topic><topic>Phagocytes - immunology</topic><topic>Phagocytes - metabolism</topic><topic>Phagocytes - parasitology</topic><topic>Recombinant Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goff, W.L</creatorcontrib><creatorcontrib>Johnson, W.C</creatorcontrib><creatorcontrib>Parish, S.M</creatorcontrib><creatorcontrib>Barrington, G.M</creatorcontrib><creatorcontrib>Elsasser, T.H</creatorcontrib><creatorcontrib>Davis, W.C</creatorcontrib><creatorcontrib>Valdez, R.A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goff, W.L</au><au>Johnson, W.C</au><au>Parish, S.M</au><au>Barrington, G.M</au><au>Elsasser, T.H</au><au>Davis, W.C</au><au>Valdez, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2002-01-15</date><risdate>2002</risdate><volume>84</volume><issue>3</issue><spage>237</spage><epage>251</epage><pages>237-251</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>The requirement for IFN-γ and/or TNF-α as co-stimulants with
Babesia
bovis merozoites for nitric oxide (NO) production was examined, as well as the regulatory role of IL-4 and IL-10. Purified
B. bovis merozoites did not induce the production of NO in undifferentiated monocytes without addition of exogenous IFN-γ and TNF-α unless the monocytes taken ex vivo were producing TNF-α endogenously. Under the latter condition, the NO production resulting from merozoite stimulation remained IFN-γ-dependent. There was no evidence for endogenous synthesis of TNF-α in monocyte-derived macrophages (MDM), and merozoites alone were incapable of inducing TNF-α mRNA in MDM. However, while merozoites plus IFN-γ induced TNF-α mRNA expression in MDM, NO was not produced. Both IL-4 and IL-10 inhibited expression of iNOS and production of NO in merozoite-stimulated monocytes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>11777537</pmid><doi>10.1016/S0165-2427(01)00413-5</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 2002-01, Vol.84 (3), p.237-251 |
| issn | 0165-2427 1873-2534 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Babesia bovis Babesia bovis - growth & development Babesia bovis - immunology Babesia bovis - pathogenicity Bovine Cattle Cell Differentiation Cytokines g-Interferon Gene Expression - drug effects In Vitro Techniques iNOS/nitric oxide Interferon-gamma - genetics Interferon-gamma - metabolism Interferon-gamma - pharmacology Interleukin-10 - pharmacology Interleukin-4 - pharmacology Macrophages - drug effects Macrophages - immunology Macrophages - metabolism Macrophages - parasitology Monocyte/macrophage Monocytes - drug effects Monocytes - immunology Monocytes - metabolism Monocytes - parasitology nitric oxide Nitric Oxide - biosynthesis Nitric Oxide Synthase - genetics Nitric Oxide Synthase - metabolism Nitric Oxide Synthase Type II Phagocytes - drug effects Phagocytes - immunology Phagocytes - metabolism Phagocytes - parasitology Recombinant Proteins RNA, Messenger - genetics RNA, Messenger - metabolism Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism Tumor Necrosis Factor-alpha - pharmacology |
| title | IL-4 and IL-10 inhibition of IFN-γ- and TNF-α-dependent nitric oxide production from bovine mononuclear phagocytes exposed to Babesia bovis merozoites |
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