IL-1β is a Better Inducer of Apoptosis in Human Fetal Membranes than IL-6
The objective of this study was to compare two of the inflammatory cytokines (IL-1 and IL-6) elevated in both preterm labour and preterm premature rupture of the membranes (pPROM), with respect to their ability to induce fetal membrane apoptosis. Fetal membranes collected from women at term were pla...
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| Veröffentlicht in: | Placenta (Eastbourne) 2003-11, Vol.24 (10), p.922-928 |
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| creator | Fortunato, S.J. Menon, R. |
| description | The objective of this study was to compare two of the inflammatory cytokines (IL-1 and IL-6) elevated in both preterm labour and preterm premature rupture of the membranes (pPROM), with respect to their ability to induce fetal membrane apoptosis. Fetal membranes collected from women at term were placed in an organ explant system and stimulated with recombinant human IL-1β and IL-6. The expression patterns of pro-apoptotic genes (Fas, FasL, TRADD, FADD) and caspases 2, 3, 8, 9 were studied using PCR. Caspase activity and DNA fragmentation were studied using substrate assays and TUNEL respectively. Caspase 8 and 9 expressions were induced in IL-1β and IL-6 treated amniochorion. Caspase 2 expression was seen only in IL-1β stimulated tissues. When compared to control, IL-1β increased caspase 2, 3, 8 and 9 activities, whereas IL-6 treated membranes did not exhibit a significant change. DNA fragmentation was seen in greater numbers after IL-1β treatment than after IL-6 treatment. This study demonstrates that IL-1β is a better inducer of apoptosis in normal human fetal membranes than IL-6. |
| doi_str_mv | 10.1016/S0143-4004(03)00160-7 |
| format | Article |
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Fetal membranes collected from women at term were placed in an organ explant system and stimulated with recombinant human IL-1β and IL-6. The expression patterns of pro-apoptotic genes (Fas, FasL, TRADD, FADD) and caspases 2, 3, 8, 9 were studied using PCR. Caspase activity and DNA fragmentation were studied using substrate assays and TUNEL respectively. Caspase 8 and 9 expressions were induced in IL-1β and IL-6 treated amniochorion. Caspase 2 expression was seen only in IL-1β stimulated tissues. When compared to control, IL-1β increased caspase 2, 3, 8 and 9 activities, whereas IL-6 treated membranes did not exhibit a significant change. DNA fragmentation was seen in greater numbers after IL-1β treatment than after IL-6 treatment. This study demonstrates that IL-1β is a better inducer of apoptosis in normal human fetal membranes than IL-6.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/S0143-4004(03)00160-7</identifier><identifier>PMID: 14580374</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Apoptosis ; Biological and medical sciences ; Caspases - metabolism ; Extraembryonic Membranes - cytology ; Extraembryonic Membranes - drug effects ; Extraembryonic Membranes - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Hormone metabolism and regulation ; Humans ; Interleukin-1 - immunology ; Interleukin-1 - pharmacology ; Interleukin-1 - physiology ; Interleukin-6 - immunology ; Interleukin-6 - pharmacology ; Interleukin-6 - physiology ; Pregnancy ; Pregnancy. Parturition. Lactation ; Vertebrates: reproduction</subject><ispartof>Placenta (Eastbourne), 2003-11, Vol.24 (10), p.922-928</ispartof><rights>2003 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-6e14b77185ed716414609f382e3d2cfd1592a43778fd12db24c5b52925731e373</citedby><cites>FETCH-LOGICAL-c443t-6e14b77185ed716414609f382e3d2cfd1592a43778fd12db24c5b52925731e373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0143-4004(03)00160-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15197801$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14580374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fortunato, S.J.</creatorcontrib><creatorcontrib>Menon, R.</creatorcontrib><title>IL-1β is a Better Inducer of Apoptosis in Human Fetal Membranes than IL-6</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>The objective of this study was to compare two of the inflammatory cytokines (IL-1 and IL-6) elevated in both preterm labour and preterm premature rupture of the membranes (pPROM), with respect to their ability to induce fetal membrane apoptosis. Fetal membranes collected from women at term were placed in an organ explant system and stimulated with recombinant human IL-1β and IL-6. The expression patterns of pro-apoptotic genes (Fas, FasL, TRADD, FADD) and caspases 2, 3, 8, 9 were studied using PCR. Caspase activity and DNA fragmentation were studied using substrate assays and TUNEL respectively. Caspase 8 and 9 expressions were induced in IL-1β and IL-6 treated amniochorion. Caspase 2 expression was seen only in IL-1β stimulated tissues. When compared to control, IL-1β increased caspase 2, 3, 8 and 9 activities, whereas IL-6 treated membranes did not exhibit a significant change. DNA fragmentation was seen in greater numbers after IL-1β treatment than after IL-6 treatment. This study demonstrates that IL-1β is a better inducer of apoptosis in normal human fetal membranes than IL-6.</description><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Caspases - metabolism</subject><subject>Extraembryonic Membranes - cytology</subject><subject>Extraembryonic Membranes - drug effects</subject><subject>Extraembryonic Membranes - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Hormone metabolism and regulation</subject><subject>Humans</subject><subject>Interleukin-1 - immunology</subject><subject>Interleukin-1 - pharmacology</subject><subject>Interleukin-1 - physiology</subject><subject>Interleukin-6 - immunology</subject><subject>Interleukin-6 - pharmacology</subject><subject>Interleukin-6 - physiology</subject><subject>Pregnancy</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Vertebrates: reproduction</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u1DAQgC0EokvhEUC-gOghZcY_cXKqSkXbrRb1UDhbjjMRRvlZ7ASJ1-qD8Ex4uyt67GlGM9_86GPsLcIpApaf7gCVLBSA-gjyBHIJCvOMrVBLUUgE8Zyt_iNH7FVKPwGgVihesiNUugJp1IrdrDcF_r3nIXHHP9M8U-TrsV18jlPHz7fTdp5S7oaRXy-DG_klza7nX2loohsp8flHLuYt5Wv2onN9ojeHeMy-X375dnFdbG6v1hfnm8IrJeeiJFSNMVhpag2WClUJdScrQbIVvmtR18IpaUyVc9E2QnndaFELbSSSNPKYfdjv3cbp10JptkNInvo-vzMtyRqUSmstM6j3oI9TSpE6u41hcPGPRbA7ifZBot0ZsiDtg0S7O_DucGBpBmofpw7WMvD-ALjkXd9lET6kR05jbSrAzJ3tOco6fgeKNvlAo6c2RPKzbafwxCv_APy6in0</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Fortunato, S.J.</creator><creator>Menon, R.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>IL-1β is a Better Inducer of Apoptosis in Human Fetal Membranes than IL-6</title><author>Fortunato, S.J. ; Menon, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-6e14b77185ed716414609f382e3d2cfd1592a43778fd12db24c5b52925731e373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Caspases - metabolism</topic><topic>Extraembryonic Membranes - cytology</topic><topic>Extraembryonic Membranes - drug effects</topic><topic>Extraembryonic Membranes - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Hormone metabolism and regulation</topic><topic>Humans</topic><topic>Interleukin-1 - immunology</topic><topic>Interleukin-1 - pharmacology</topic><topic>Interleukin-1 - physiology</topic><topic>Interleukin-6 - immunology</topic><topic>Interleukin-6 - pharmacology</topic><topic>Interleukin-6 - physiology</topic><topic>Pregnancy</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fortunato, S.J.</creatorcontrib><creatorcontrib>Menon, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fortunato, S.J.</au><au>Menon, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IL-1β is a Better Inducer of Apoptosis in Human Fetal Membranes than IL-6</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>24</volume><issue>10</issue><spage>922</spage><epage>928</epage><pages>922-928</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>The objective of this study was to compare two of the inflammatory cytokines (IL-1 and IL-6) elevated in both preterm labour and preterm premature rupture of the membranes (pPROM), with respect to their ability to induce fetal membrane apoptosis. Fetal membranes collected from women at term were placed in an organ explant system and stimulated with recombinant human IL-1β and IL-6. The expression patterns of pro-apoptotic genes (Fas, FasL, TRADD, FADD) and caspases 2, 3, 8, 9 were studied using PCR. Caspase activity and DNA fragmentation were studied using substrate assays and TUNEL respectively. Caspase 8 and 9 expressions were induced in IL-1β and IL-6 treated amniochorion. Caspase 2 expression was seen only in IL-1β stimulated tissues. When compared to control, IL-1β increased caspase 2, 3, 8 and 9 activities, whereas IL-6 treated membranes did not exhibit a significant change. DNA fragmentation was seen in greater numbers after IL-1β treatment than after IL-6 treatment. This study demonstrates that IL-1β is a better inducer of apoptosis in normal human fetal membranes than IL-6.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>14580374</pmid><doi>10.1016/S0143-4004(03)00160-7</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Apoptosis Biological and medical sciences Caspases - metabolism Extraembryonic Membranes - cytology Extraembryonic Membranes - drug effects Extraembryonic Membranes - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Hormone metabolism and regulation Humans Interleukin-1 - immunology Interleukin-1 - pharmacology Interleukin-1 - physiology Interleukin-6 - immunology Interleukin-6 - pharmacology Interleukin-6 - physiology Pregnancy Pregnancy. Parturition. Lactation Vertebrates: reproduction |
| title | IL-1β is a Better Inducer of Apoptosis in Human Fetal Membranes than IL-6 |
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