Defects in cholangiocyte fibrocystin expression and ciliary structure in the PCK rat
Recent studies have showed that proteins associated with polycystic kidney disease (PKD) are expressed in cilia, linking this organelle and cyst formation in the kidney, but involvement of cilia in PKD-related biliary cystogenesis has not been shown. We investigated: (1) the expression of fibrocysti...
Gespeichert in:
| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 2003-11, Vol.125 (5), p.1303-1310 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1310 |
|---|---|
| container_issue | 5 |
| container_start_page | 1303 |
| container_title | Gastroenterology (New York, N.Y. 1943) |
| container_volume | 125 |
| creator | Masyuk, Tatyana V Huang, Bing Q Ward, Christopher J Masyuk, Anatoliy I Yuan, David Splinter, Patrick L Punyashthiti, Rachaneekorn Ritman, Eric L Torres, Vicente E Harris, Peter C Larusso, Nicholas F |
| description | Recent studies have showed that proteins associated with polycystic kidney disease (PKD) are expressed in cilia, linking this organelle and cyst formation in the kidney, but involvement of cilia in PKD-related biliary cystogenesis has not been shown. We investigated: (1) the expression of fibrocystin (a product of
PKHD1, the autosomal-recessive PKD [ARPKD] gene) in cholangiocyte cilia; (2) biliary cyst formation in an orthologous rat model, PCK; and (3) the effect of
Pkhd1 mutation on ciliary structure.
Biliary cystogenesis was assessed by microcomputed tomography. Fibrocystin expression in cholangiocytes of isolated intrahepatic bile ducts (IBDUs) and liver cysts was analyzed by confocal and immunoelectron microscopy, and ciliary structure and length by scanning and transmission electron microscopy. Small interfering RNAs (siRNA) were used to examine the effect of fibrocystin loss on ciliary structure.
The biliary tree in the PCK rat was distorted markedly, showing multiple bile duct dilatation and focal budding. In normal IBDUs, each cholangiocyte had a single cilium that expressed fibrocystin. In contrast, cilia in the PCK rat were abnormal with bulbous extensions and diminished length, and were devoid of fibrocystin. In cholangiocytes of normal IBDUs, specific siRNA reduced
Pkhd1 messenger RNA by 80%, the length of cilia by 41%, and fibrocystin ciliary expression to an undetectable level.
Our results indicate that fibrocystin is expressed in cholangiocyte cilia and that disruption of
Pkhd1 by a germ line mutation in the PCK rat or by siRNA in IBDUs results in abnormalities in ciliary morphology and possibly biliary cystogenesis. |
| doi_str_mv | 10.1016/j.gastro.2003.09.001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71341102</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0016508503013696</els_id><sourcerecordid>71341102</sourcerecordid><originalsourceid>FETCH-LOGICAL-e292t-16d386e6f7689d3f72659f7654633eaae191366f435aa9663eb13491628ab8af3</originalsourceid><addsrcrecordid>eNpFkctOwzAQRS0EoqXwBwhlA7sEPxIn3iCh8hSVYFHWluOMW1dpUmwH0b_HVYtYzVhzxqN7L0KXBGcEE367yhbKB9dnFGOWYZFhTI7QmBS0SmNLj9E4Fp4WuCpG6Mz7FcZYsIqcohHJC1HRnI_R_AEM6OAT2yV62beqW9hebwMkxtYudj7ECfxsHHhv-y5RXZNo21rltkk8P-gwONhthyUkH9O3xKlwjk6Maj1cHOoEfT49zqcv6ez9-XV6P0uBChpSwhtWceCm5JVomCkpL0R8FDlnDJQCIgjj3OSsUEpwzqAmLBeE00rVlTJsgm72_25c_zWAD3JtvYY2qoB-8LKMOCGYRvDqAA71Ghq5cXYdBcg_HyJwfQCU16o1TnXa-n8umspxUUbubs9BlPVtwUmvLXQaGuuijbLprSRY7gKSK7kPSO4CkljIGAf7BYHFgyw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71341102</pqid></control><display><type>article</type><title>Defects in cholangiocyte fibrocystin expression and ciliary structure in the PCK rat</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><source>Alma/SFX Local Collection</source><creator>Masyuk, Tatyana V ; Huang, Bing Q ; Ward, Christopher J ; Masyuk, Anatoliy I ; Yuan, David ; Splinter, Patrick L ; Punyashthiti, Rachaneekorn ; Ritman, Eric L ; Torres, Vicente E ; Harris, Peter C ; Larusso, Nicholas F</creator><creatorcontrib>Masyuk, Tatyana V ; Huang, Bing Q ; Ward, Christopher J ; Masyuk, Anatoliy I ; Yuan, David ; Splinter, Patrick L ; Punyashthiti, Rachaneekorn ; Ritman, Eric L ; Torres, Vicente E ; Harris, Peter C ; Larusso, Nicholas F</creatorcontrib><description>Recent studies have showed that proteins associated with polycystic kidney disease (PKD) are expressed in cilia, linking this organelle and cyst formation in the kidney, but involvement of cilia in PKD-related biliary cystogenesis has not been shown. We investigated: (1) the expression of fibrocystin (a product of
PKHD1, the autosomal-recessive PKD [ARPKD] gene) in cholangiocyte cilia; (2) biliary cyst formation in an orthologous rat model, PCK; and (3) the effect of
Pkhd1 mutation on ciliary structure.
Biliary cystogenesis was assessed by microcomputed tomography. Fibrocystin expression in cholangiocytes of isolated intrahepatic bile ducts (IBDUs) and liver cysts was analyzed by confocal and immunoelectron microscopy, and ciliary structure and length by scanning and transmission electron microscopy. Small interfering RNAs (siRNA) were used to examine the effect of fibrocystin loss on ciliary structure.
The biliary tree in the PCK rat was distorted markedly, showing multiple bile duct dilatation and focal budding. In normal IBDUs, each cholangiocyte had a single cilium that expressed fibrocystin. In contrast, cilia in the PCK rat were abnormal with bulbous extensions and diminished length, and were devoid of fibrocystin. In cholangiocytes of normal IBDUs, specific siRNA reduced
Pkhd1 messenger RNA by 80%, the length of cilia by 41%, and fibrocystin ciliary expression to an undetectable level.
Our results indicate that fibrocystin is expressed in cholangiocyte cilia and that disruption of
Pkhd1 by a germ line mutation in the PCK rat or by siRNA in IBDUs results in abnormalities in ciliary morphology and possibly biliary cystogenesis.</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1016/j.gastro.2003.09.001</identifier><identifier>PMID: 14598246</identifier><identifier>CODEN: GASTAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; ARPKD ; autosomal-recessive polycystic kidney disease ; Bile Ducts - metabolism ; Bile Ducts - pathology ; Bile Ducts, Intrahepatic - metabolism ; Biological and medical sciences ; Blotting, Western ; Cilia - metabolism ; Cilia - ultrastructure ; Disease Models, Animal ; FB2 ; Fluorescent Antibody Technique ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Silencing ; Germ-Line Mutation ; IBDU ; IEM ; immunoelectron microscopy ; isolated intrahepatic bile duct ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Medical sciences ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Microscopy, Immunoelectron ; Other diseases. Semiology ; PKD ; polycystic kidney disease ; Polycystic Kidney, Autosomal Recessive - genetics ; Polycystic Kidney, Autosomal Recessive - metabolism ; Polycystic Kidney, Autosomal Recessive - pathology ; primary monoclonal antibody ; Rats ; Rats, Mutant Strains ; Receptors, Cell Surface - deficiency ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; RNA, Small Interfering - metabolism ; scanning electron microscopy ; SEM ; siRNA ; small interfering RNA ; TEM ; transmission electron microscopy</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 2003-11, Vol.125 (5), p.1303-1310</ispartof><rights>2003 American Gastroenterological Association</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gastro.2003.09.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15286057$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14598246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Masyuk, Tatyana V</creatorcontrib><creatorcontrib>Huang, Bing Q</creatorcontrib><creatorcontrib>Ward, Christopher J</creatorcontrib><creatorcontrib>Masyuk, Anatoliy I</creatorcontrib><creatorcontrib>Yuan, David</creatorcontrib><creatorcontrib>Splinter, Patrick L</creatorcontrib><creatorcontrib>Punyashthiti, Rachaneekorn</creatorcontrib><creatorcontrib>Ritman, Eric L</creatorcontrib><creatorcontrib>Torres, Vicente E</creatorcontrib><creatorcontrib>Harris, Peter C</creatorcontrib><creatorcontrib>Larusso, Nicholas F</creatorcontrib><title>Defects in cholangiocyte fibrocystin expression and ciliary structure in the PCK rat</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>Recent studies have showed that proteins associated with polycystic kidney disease (PKD) are expressed in cilia, linking this organelle and cyst formation in the kidney, but involvement of cilia in PKD-related biliary cystogenesis has not been shown. We investigated: (1) the expression of fibrocystin (a product of
PKHD1, the autosomal-recessive PKD [ARPKD] gene) in cholangiocyte cilia; (2) biliary cyst formation in an orthologous rat model, PCK; and (3) the effect of
Pkhd1 mutation on ciliary structure.
Biliary cystogenesis was assessed by microcomputed tomography. Fibrocystin expression in cholangiocytes of isolated intrahepatic bile ducts (IBDUs) and liver cysts was analyzed by confocal and immunoelectron microscopy, and ciliary structure and length by scanning and transmission electron microscopy. Small interfering RNAs (siRNA) were used to examine the effect of fibrocystin loss on ciliary structure.
The biliary tree in the PCK rat was distorted markedly, showing multiple bile duct dilatation and focal budding. In normal IBDUs, each cholangiocyte had a single cilium that expressed fibrocystin. In contrast, cilia in the PCK rat were abnormal with bulbous extensions and diminished length, and were devoid of fibrocystin. In cholangiocytes of normal IBDUs, specific siRNA reduced
Pkhd1 messenger RNA by 80%, the length of cilia by 41%, and fibrocystin ciliary expression to an undetectable level.
Our results indicate that fibrocystin is expressed in cholangiocyte cilia and that disruption of
Pkhd1 by a germ line mutation in the PCK rat or by siRNA in IBDUs results in abnormalities in ciliary morphology and possibly biliary cystogenesis.</description><subject>Animals</subject><subject>ARPKD</subject><subject>autosomal-recessive polycystic kidney disease</subject><subject>Bile Ducts - metabolism</subject><subject>Bile Ducts - pathology</subject><subject>Bile Ducts, Intrahepatic - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cilia - metabolism</subject><subject>Cilia - ultrastructure</subject><subject>Disease Models, Animal</subject><subject>FB2</subject><subject>Fluorescent Antibody Technique</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Silencing</subject><subject>Germ-Line Mutation</subject><subject>IBDU</subject><subject>IEM</subject><subject>immunoelectron microscopy</subject><subject>isolated intrahepatic bile duct</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Immunoelectron</subject><subject>Other diseases. Semiology</subject><subject>PKD</subject><subject>polycystic kidney disease</subject><subject>Polycystic Kidney, Autosomal Recessive - genetics</subject><subject>Polycystic Kidney, Autosomal Recessive - metabolism</subject><subject>Polycystic Kidney, Autosomal Recessive - pathology</subject><subject>primary monoclonal antibody</subject><subject>Rats</subject><subject>Rats, Mutant Strains</subject><subject>Receptors, Cell Surface - deficiency</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>RNA, Small Interfering - metabolism</subject><subject>scanning electron microscopy</subject><subject>SEM</subject><subject>siRNA</subject><subject>small interfering RNA</subject><subject>TEM</subject><subject>transmission electron microscopy</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkctOwzAQRS0EoqXwBwhlA7sEPxIn3iCh8hSVYFHWluOMW1dpUmwH0b_HVYtYzVhzxqN7L0KXBGcEE367yhbKB9dnFGOWYZFhTI7QmBS0SmNLj9E4Fp4WuCpG6Mz7FcZYsIqcohHJC1HRnI_R_AEM6OAT2yV62beqW9hebwMkxtYudj7ECfxsHHhv-y5RXZNo21rltkk8P-gwONhthyUkH9O3xKlwjk6Maj1cHOoEfT49zqcv6ez9-XV6P0uBChpSwhtWceCm5JVomCkpL0R8FDlnDJQCIgjj3OSsUEpwzqAmLBeE00rVlTJsgm72_25c_zWAD3JtvYY2qoB-8LKMOCGYRvDqAA71Ghq5cXYdBcg_HyJwfQCU16o1TnXa-n8umspxUUbubs9BlPVtwUmvLXQaGuuijbLprSRY7gKSK7kPSO4CkljIGAf7BYHFgyw</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Masyuk, Tatyana V</creator><creator>Huang, Bing Q</creator><creator>Ward, Christopher J</creator><creator>Masyuk, Anatoliy I</creator><creator>Yuan, David</creator><creator>Splinter, Patrick L</creator><creator>Punyashthiti, Rachaneekorn</creator><creator>Ritman, Eric L</creator><creator>Torres, Vicente E</creator><creator>Harris, Peter C</creator><creator>Larusso, Nicholas F</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Defects in cholangiocyte fibrocystin expression and ciliary structure in the PCK rat</title><author>Masyuk, Tatyana V ; Huang, Bing Q ; Ward, Christopher J ; Masyuk, Anatoliy I ; Yuan, David ; Splinter, Patrick L ; Punyashthiti, Rachaneekorn ; Ritman, Eric L ; Torres, Vicente E ; Harris, Peter C ; Larusso, Nicholas F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e292t-16d386e6f7689d3f72659f7654633eaae191366f435aa9663eb13491628ab8af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>ARPKD</topic><topic>autosomal-recessive polycystic kidney disease</topic><topic>Bile Ducts - metabolism</topic><topic>Bile Ducts - pathology</topic><topic>Bile Ducts, Intrahepatic - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cilia - metabolism</topic><topic>Cilia - ultrastructure</topic><topic>Disease Models, Animal</topic><topic>FB2</topic><topic>Fluorescent Antibody Technique</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Silencing</topic><topic>Germ-Line Mutation</topic><topic>IBDU</topic><topic>IEM</topic><topic>immunoelectron microscopy</topic><topic>isolated intrahepatic bile duct</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Immunoelectron</topic><topic>Other diseases. Semiology</topic><topic>PKD</topic><topic>polycystic kidney disease</topic><topic>Polycystic Kidney, Autosomal Recessive - genetics</topic><topic>Polycystic Kidney, Autosomal Recessive - metabolism</topic><topic>Polycystic Kidney, Autosomal Recessive - pathology</topic><topic>primary monoclonal antibody</topic><topic>Rats</topic><topic>Rats, Mutant Strains</topic><topic>Receptors, Cell Surface - deficiency</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>RNA, Small Interfering - metabolism</topic><topic>scanning electron microscopy</topic><topic>SEM</topic><topic>siRNA</topic><topic>small interfering RNA</topic><topic>TEM</topic><topic>transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masyuk, Tatyana V</creatorcontrib><creatorcontrib>Huang, Bing Q</creatorcontrib><creatorcontrib>Ward, Christopher J</creatorcontrib><creatorcontrib>Masyuk, Anatoliy I</creatorcontrib><creatorcontrib>Yuan, David</creatorcontrib><creatorcontrib>Splinter, Patrick L</creatorcontrib><creatorcontrib>Punyashthiti, Rachaneekorn</creatorcontrib><creatorcontrib>Ritman, Eric L</creatorcontrib><creatorcontrib>Torres, Vicente E</creatorcontrib><creatorcontrib>Harris, Peter C</creatorcontrib><creatorcontrib>Larusso, Nicholas F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masyuk, Tatyana V</au><au>Huang, Bing Q</au><au>Ward, Christopher J</au><au>Masyuk, Anatoliy I</au><au>Yuan, David</au><au>Splinter, Patrick L</au><au>Punyashthiti, Rachaneekorn</au><au>Ritman, Eric L</au><au>Torres, Vicente E</au><au>Harris, Peter C</au><au>Larusso, Nicholas F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defects in cholangiocyte fibrocystin expression and ciliary structure in the PCK rat</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>125</volume><issue>5</issue><spage>1303</spage><epage>1310</epage><pages>1303-1310</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>Recent studies have showed that proteins associated with polycystic kidney disease (PKD) are expressed in cilia, linking this organelle and cyst formation in the kidney, but involvement of cilia in PKD-related biliary cystogenesis has not been shown. We investigated: (1) the expression of fibrocystin (a product of
PKHD1, the autosomal-recessive PKD [ARPKD] gene) in cholangiocyte cilia; (2) biliary cyst formation in an orthologous rat model, PCK; and (3) the effect of
Pkhd1 mutation on ciliary structure.
Biliary cystogenesis was assessed by microcomputed tomography. Fibrocystin expression in cholangiocytes of isolated intrahepatic bile ducts (IBDUs) and liver cysts was analyzed by confocal and immunoelectron microscopy, and ciliary structure and length by scanning and transmission electron microscopy. Small interfering RNAs (siRNA) were used to examine the effect of fibrocystin loss on ciliary structure.
The biliary tree in the PCK rat was distorted markedly, showing multiple bile duct dilatation and focal budding. In normal IBDUs, each cholangiocyte had a single cilium that expressed fibrocystin. In contrast, cilia in the PCK rat were abnormal with bulbous extensions and diminished length, and were devoid of fibrocystin. In cholangiocytes of normal IBDUs, specific siRNA reduced
Pkhd1 messenger RNA by 80%, the length of cilia by 41%, and fibrocystin ciliary expression to an undetectable level.
Our results indicate that fibrocystin is expressed in cholangiocyte cilia and that disruption of
Pkhd1 by a germ line mutation in the PCK rat or by siRNA in IBDUs results in abnormalities in ciliary morphology and possibly biliary cystogenesis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>14598246</pmid><doi>10.1016/j.gastro.2003.09.001</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0016-5085 |
| ispartof | Gastroenterology (New York, N.Y. 1943), 2003-11, Vol.125 (5), p.1303-1310 |
| issn | 0016-5085 1528-0012 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71341102 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Alma/SFX Local Collection |
| subjects | Animals ARPKD autosomal-recessive polycystic kidney disease Bile Ducts - metabolism Bile Ducts - pathology Bile Ducts, Intrahepatic - metabolism Biological and medical sciences Blotting, Western Cilia - metabolism Cilia - ultrastructure Disease Models, Animal FB2 Fluorescent Antibody Technique Gastroenterology. Liver. Pancreas. Abdomen Gene Silencing Germ-Line Mutation IBDU IEM immunoelectron microscopy isolated intrahepatic bile duct Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences Microscopy, Electron Microscopy, Electron, Scanning Microscopy, Immunoelectron Other diseases. Semiology PKD polycystic kidney disease Polycystic Kidney, Autosomal Recessive - genetics Polycystic Kidney, Autosomal Recessive - metabolism Polycystic Kidney, Autosomal Recessive - pathology primary monoclonal antibody Rats Rats, Mutant Strains Receptors, Cell Surface - deficiency Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism RNA, Small Interfering - metabolism scanning electron microscopy SEM siRNA small interfering RNA TEM transmission electron microscopy |
| title | Defects in cholangiocyte fibrocystin expression and ciliary structure in the PCK rat |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T05%3A49%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Defects%20in%20cholangiocyte%20fibrocystin%20expression%20and%20ciliary%20structure%20in%20the%20PCK%20rat&rft.jtitle=Gastroenterology%20(New%20York,%20N.Y.%201943)&rft.au=Masyuk,%20Tatyana%20V&rft.date=2003-11-01&rft.volume=125&rft.issue=5&rft.spage=1303&rft.epage=1310&rft.pages=1303-1310&rft.issn=0016-5085&rft.eissn=1528-0012&rft.coden=GASTAB&rft_id=info:doi/10.1016/j.gastro.2003.09.001&rft_dat=%3Cproquest_pubme%3E71341102%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71341102&rft_id=info:pmid/14598246&rft_els_id=S0016508503013696&rfr_iscdi=true |