Hydrogen peroxide generation with immobilized glucose oxidase for textile bleaching

Glucose oxidase was covalently immobilized on commercially available alumina and glass supports, with a high level of protein recovery. The operational stability of the alumina carrier was an advantage over the glass support, though the rate of generation of hydrogen peroxide in the case of the latt...

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Veröffentlicht in:Journal of biotechnology 2002-01, Vol.93 (1), p.87-94
Hauptverfasser: Tzanov, Tzanko, Costa, Silgia A, Gübitz, Georg M, Cavaco-Paulo, Artur
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container_issue 1
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container_title Journal of biotechnology
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creator Tzanov, Tzanko
Costa, Silgia A
Gübitz, Georg M
Cavaco-Paulo, Artur
description Glucose oxidase was covalently immobilized on commercially available alumina and glass supports, with a high level of protein recovery. The operational stability of the alumina carrier was an advantage over the glass support, though the rate of generation of hydrogen peroxide in the case of the latter was higher. The immobilization technique provided repeated application of the enzyme even in low concentration, and the hydrogen peroxide generated in the enzymatic reaction was successively used for textile bleaching.
doi_str_mv 10.1016/S0168-1656(01)00386-8
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Alumina
Aluminum Oxide
Aspergillus niger - enzymology
Biological and medical sciences
Biotechnology
Cellulose
Coloring Agents - isolation & purification
Enzymes, Immobilized - metabolism
Fundamental and applied biological sciences. Psychology
Glass
Glucose
Glucose oxidase
Glucose Oxidase - metabolism
Gossypium
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Immobilization
Proteins
Textile bleaching
Textiles
title Hydrogen peroxide generation with immobilized glucose oxidase for textile bleaching
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