Expression of a sodium bicarbonate cotransporter in human parotid salivary glands
The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na +–HCO 3 − cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in is...
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| Veröffentlicht in: | Archives of oral biology 2002, Vol.47 (1), p.1-9 |
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| creator | Park, K Hurley, P.T Roussa, E Cooper, G.J Smith, C.P Thévenod, F Steward, M.C Case, R.M |
| description | The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na
+–HCO
3
− cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO
3
− uptake via a Na
+-dependent, DIDS-sensitive mechanism. By reverse transcriptase–polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in
Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na
+- and HCO
3
−-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na
+–HCO
3
− cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion. |
| doi_str_mv | 10.1016/S0003-9969(01)00098-X |
| format | Article |
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+–HCO
3
− cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO
3
− uptake via a Na
+-dependent, DIDS-sensitive mechanism. By reverse transcriptase–polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in
Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na
+- and HCO
3
−-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na
+–HCO
3
− cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/S0003-9969(01)00098-X</identifier><identifier>PMID: 11743927</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology ; Adult ; Aged ; Amiloride - pharmacology ; Ammonium Chloride - pharmacology ; Animals ; Bicarbonate secretion ; Cell Membrane - metabolism ; Cloning, Molecular ; Cytophotometry ; Dentistry ; Diuretics - pharmacology ; Female ; Gene Expression ; Humans ; Hydrogen-Ion Concentration ; Immunohistochemistry ; Intracellular pH ; Male ; Membrane Potentials - physiology ; Microelectrodes ; Middle Aged ; NBC ; Oocytes - metabolism ; Parotid Gland - cytology ; Parotid Gland - metabolism ; Protein Isoforms - antagonists & inhibitors ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Sodium - metabolism ; Sodium - pharmacology ; Sodium-Bicarbonate Symporters - antagonists & inhibitors ; Sodium-Bicarbonate Symporters - genetics ; Sodium-Bicarbonate Symporters - metabolism ; Sodium-Hydrogen Exchangers - antagonists & inhibitors ; Sodium-Hydrogen Exchangers - metabolism ; Statistics as Topic ; Xenopus laevis</subject><ispartof>Archives of oral biology, 2002, Vol.47 (1), p.1-9</ispartof><rights>2002 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-84487ad950aee34bc880ab7328234accde81d964180569035f3cc1f633f9bf323</citedby><cites>FETCH-LOGICAL-c427t-84487ad950aee34bc880ab7328234accde81d964180569035f3cc1f633f9bf323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0003-9969(01)00098-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,4026,27930,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11743927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, K</creatorcontrib><creatorcontrib>Hurley, P.T</creatorcontrib><creatorcontrib>Roussa, E</creatorcontrib><creatorcontrib>Cooper, G.J</creatorcontrib><creatorcontrib>Smith, C.P</creatorcontrib><creatorcontrib>Thévenod, F</creatorcontrib><creatorcontrib>Steward, M.C</creatorcontrib><creatorcontrib>Case, R.M</creatorcontrib><title>Expression of a sodium bicarbonate cotransporter in human parotid salivary glands</title><title>Archives of oral biology</title><addtitle>Arch Oral Biol</addtitle><description>The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na
+–HCO
3
− cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO
3
− uptake via a Na
+-dependent, DIDS-sensitive mechanism. By reverse transcriptase–polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in
Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na
+- and HCO
3
−-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na
+–HCO
3
− cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion.</description><subject>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology</subject><subject>Adult</subject><subject>Aged</subject><subject>Amiloride - pharmacology</subject><subject>Ammonium Chloride - pharmacology</subject><subject>Animals</subject><subject>Bicarbonate secretion</subject><subject>Cell Membrane - metabolism</subject><subject>Cloning, Molecular</subject><subject>Cytophotometry</subject><subject>Dentistry</subject><subject>Diuretics - pharmacology</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunohistochemistry</subject><subject>Intracellular pH</subject><subject>Male</subject><subject>Membrane Potentials - physiology</subject><subject>Microelectrodes</subject><subject>Middle Aged</subject><subject>NBC</subject><subject>Oocytes - metabolism</subject><subject>Parotid Gland - cytology</subject><subject>Parotid Gland - metabolism</subject><subject>Protein Isoforms - antagonists & inhibitors</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - metabolism</subject><subject>Sodium - metabolism</subject><subject>Sodium - pharmacology</subject><subject>Sodium-Bicarbonate Symporters - antagonists & inhibitors</subject><subject>Sodium-Bicarbonate Symporters - genetics</subject><subject>Sodium-Bicarbonate Symporters - metabolism</subject><subject>Sodium-Hydrogen Exchangers - antagonists & inhibitors</subject><subject>Sodium-Hydrogen Exchangers - metabolism</subject><subject>Statistics as Topic</subject><subject>Xenopus laevis</subject><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rr6E5ScRA_VpOlHchJZ1g8QRFTYW0iTqUa2TU3aRf-92Q_06GkYeGbmnQehY0ouKKHF5TMhhCVCFOKM0PPYCJ7Md9CY8lIkNCfFLhr_IiN0EMJHbPOioPtoRGmZMZGWY_Q0--o8hGBdi12NFQ7O2KHBldXKV65VPWDteq_a0Dnfg8e2xe9Do1rcKe96a3BQC7tU_hu_LVRrwiHaq9UiwNG2TtDrzexlepc8PN7eT68fEp2lZZ_wLOOlMiInCoBlleacqKpkKU9ZprQ2wKkRRUZ5DC0Iy2umNa0LxmpR1SxlE3S62dt59zlA6GVjg4ZFDAFuCLKkjPH4ZgTzDai9C8FDLTtvmxhYUiJXLuXapVyJkoTKtUs5j3Mn2wND1YD5m9rKi8DVBoD45tKCl0FbaDUY60H30jj7z4kfJxmElA</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Park, K</creator><creator>Hurley, P.T</creator><creator>Roussa, E</creator><creator>Cooper, G.J</creator><creator>Smith, C.P</creator><creator>Thévenod, F</creator><creator>Steward, M.C</creator><creator>Case, R.M</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Expression of a sodium bicarbonate cotransporter in human parotid salivary glands</title><author>Park, K ; Hurley, P.T ; Roussa, E ; Cooper, G.J ; Smith, C.P ; Thévenod, F ; Steward, M.C ; Case, R.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-84487ad950aee34bc880ab7328234accde81d964180569035f3cc1f633f9bf323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology</topic><topic>Adult</topic><topic>Aged</topic><topic>Amiloride - pharmacology</topic><topic>Ammonium Chloride - pharmacology</topic><topic>Animals</topic><topic>Bicarbonate secretion</topic><topic>Cell Membrane - metabolism</topic><topic>Cloning, Molecular</topic><topic>Cytophotometry</topic><topic>Dentistry</topic><topic>Diuretics - pharmacology</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunohistochemistry</topic><topic>Intracellular pH</topic><topic>Male</topic><topic>Membrane Potentials - physiology</topic><topic>Microelectrodes</topic><topic>Middle Aged</topic><topic>NBC</topic><topic>Oocytes - metabolism</topic><topic>Parotid Gland - cytology</topic><topic>Parotid Gland - metabolism</topic><topic>Protein Isoforms - antagonists & inhibitors</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - metabolism</topic><topic>Sodium - metabolism</topic><topic>Sodium - pharmacology</topic><topic>Sodium-Bicarbonate Symporters - antagonists & inhibitors</topic><topic>Sodium-Bicarbonate Symporters - genetics</topic><topic>Sodium-Bicarbonate Symporters - metabolism</topic><topic>Sodium-Hydrogen Exchangers - antagonists & inhibitors</topic><topic>Sodium-Hydrogen Exchangers - metabolism</topic><topic>Statistics as Topic</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, K</creatorcontrib><creatorcontrib>Hurley, P.T</creatorcontrib><creatorcontrib>Roussa, E</creatorcontrib><creatorcontrib>Cooper, G.J</creatorcontrib><creatorcontrib>Smith, C.P</creatorcontrib><creatorcontrib>Thévenod, F</creatorcontrib><creatorcontrib>Steward, M.C</creatorcontrib><creatorcontrib>Case, R.M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, K</au><au>Hurley, P.T</au><au>Roussa, E</au><au>Cooper, G.J</au><au>Smith, C.P</au><au>Thévenod, F</au><au>Steward, M.C</au><au>Case, R.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a sodium bicarbonate cotransporter in human parotid salivary glands</atitle><jtitle>Archives of oral biology</jtitle><addtitle>Arch Oral Biol</addtitle><date>2002</date><risdate>2002</risdate><volume>47</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>The human parotid gland secretes much of the bicarbonate that enters the mouth. Prompted by studies of animal models, this study sought evidence for the expression of a functional Na
+–HCO
3
− cotransporter (NBC) in human parotid acinar cells. Microfluorometric measurements of intracellular pH in isolated acini showed that the recovery from an acid load was achieved in part by HCO
3
− uptake via a Na
+-dependent, DIDS-sensitive mechanism. By reverse transcriptase–polymerase chain reaction, a full-length NBC1 clone was obtained showing more than 99% homology with the human pancreatic isoform hpNBC1. Expressed in
Xenopus oocytes, the electrogenicity of the transporter was detected as an inwardly directed, Na
+- and HCO
3
−-dependent flux of negative charge. Immunohistochemistry using antibodies raised to NBC1 showed strong staining of the basolateral membrane of the acinar cells. Therefore, it was concluded that a functional electrogenic Na
+–HCO
3
− cotransporter is expressed in the human parotid gland, and that it contributes to pH regulation in the acinar cells and could play a significant part in salivary secretion.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11743927</pmid><doi>10.1016/S0003-9969(01)00098-X</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Archives of oral biology, 2002, Vol.47 (1), p.1-9 |
| issn | 0003-9969 1879-1506 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology Adult Aged Amiloride - pharmacology Ammonium Chloride - pharmacology Animals Bicarbonate secretion Cell Membrane - metabolism Cloning, Molecular Cytophotometry Dentistry Diuretics - pharmacology Female Gene Expression Humans Hydrogen-Ion Concentration Immunohistochemistry Intracellular pH Male Membrane Potentials - physiology Microelectrodes Middle Aged NBC Oocytes - metabolism Parotid Gland - cytology Parotid Gland - metabolism Protein Isoforms - antagonists & inhibitors Protein Isoforms - genetics Protein Isoforms - metabolism Sodium - metabolism Sodium - pharmacology Sodium-Bicarbonate Symporters - antagonists & inhibitors Sodium-Bicarbonate Symporters - genetics Sodium-Bicarbonate Symporters - metabolism Sodium-Hydrogen Exchangers - antagonists & inhibitors Sodium-Hydrogen Exchangers - metabolism Statistics as Topic Xenopus laevis |
| title | Expression of a sodium bicarbonate cotransporter in human parotid salivary glands |
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