Regulation of expression of two novel flower‐specific genes from tomato (Solanum lycopersicum) by gibberellin
Two flower‐specific cDNAs have been isolated after differential screening of an anther cDNA library. This library was constructed 48 h after GA3 treatment of buds of the GA‐deficient gib‐1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression o...
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| Veröffentlicht in: | Journal of experimental botany 2002-01, Vol.53 (366), p.51-59 |
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| container_title | Journal of experimental botany |
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| creator | Van den Heuvel, Koen J. Van Lipzig, Rosalinde H. Barendse, Gerard W. Wullems, George J. |
| description | Two flower‐specific cDNAs have been isolated after differential screening of an anther cDNA library. This library was constructed 48 h after GA3 treatment of buds of the GA‐deficient gib‐1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression of both genes is regulated by gibberellins (GAs). Application of GA3 to developmentally arrested gib‐1 flower buds induced new expression of tgas100 mRNA 48 h post‐treatment, while an increased accumulation of tgas105 mRNA was found after 8 h. In situ analyses showed the spatial distribution of the expression of both genes within the tomato flower. One of the deduced polypeptides (TGAS105) displays similarities to cysteine‐rich extensin‐like proteins, while the other (TGAS100) shows significant homology with a stamen‐specific gene of Antirrhinum majus. Based on the deduced protein sequences, the possible function of the encoded proteins is discussed. |
| doi_str_mv | 10.1093/jexbot/53.366.51 |
| format | Article |
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This library was constructed 48 h after GA3 treatment of buds of the GA‐deficient gib‐1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression of both genes is regulated by gibberellins (GAs). Application of GA3 to developmentally arrested gib‐1 flower buds induced new expression of tgas100 mRNA 48 h post‐treatment, while an increased accumulation of tgas105 mRNA was found after 8 h. In situ analyses showed the spatial distribution of the expression of both genes within the tomato flower. One of the deduced polypeptides (TGAS105) displays similarities to cysteine‐rich extensin‐like proteins, while the other (TGAS100) shows significant homology with a stamen‐specific gene of Antirrhinum majus. Based on the deduced protein sequences, the possible function of the encoded proteins is discussed.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jexbot/53.366.51</identifier><identifier>PMID: 11741041</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Agronomy. Soil science and plant productions ; Amino Acid Sequence ; Anthers ; Antirrhinum majus ; Biological and medical sciences ; Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation ; Blotting, Northern ; Cell and Molecular Biology, Biochemistry and Molecular Physiology ; Complementary DNA ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; Economic plant physiology ; Extensin‐like ; Flower buds ; Flowering ; Flowering, floral biology, reproduction patterns ; flower‐specific gene expression ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Plant - drug effects ; gib-1 gene ; gibberellin ; Gibberellins ; Gibberellins - pharmacology ; Growth and development ; In Situ Hybridization ; Lycopersicon esculentum ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - growth & development ; Messenger RNA ; Molecular Sequence Data ; Mutation ; Plant physiology and development ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Stems - genetics ; Plant Stems - growth & development ; Plants ; Proteins ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Solanum lycopersicum ; tgas100 gene ; Tissue Distribution ; Vegetative and sexual reproduction, floral biology, fructification</subject><ispartof>Journal of experimental botany, 2002-01, Vol.53 (366), p.51-59</ispartof><rights>Society for Experimental Biology 2002</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-3dcb3b76f2733823cb56114e39b377f3e35f7cfebe035d07ae578d80fcd0c5b23</citedby><cites>FETCH-LOGICAL-c524t-3dcb3b76f2733823cb56114e39b377f3e35f7cfebe035d07ae578d80fcd0c5b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23697266$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23697266$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13531956$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11741041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van den Heuvel, Koen J.</creatorcontrib><creatorcontrib>Van Lipzig, Rosalinde H.</creatorcontrib><creatorcontrib>Barendse, Gerard W.</creatorcontrib><creatorcontrib>Wullems, George J.</creatorcontrib><title>Regulation of expression of two novel flower‐specific genes from tomato (Solanum lycopersicum) by gibberellin</title><title>Journal of experimental botany</title><addtitle>J. Exp. Bot</addtitle><description>Two flower‐specific cDNAs have been isolated after differential screening of an anther cDNA library. This library was constructed 48 h after GA3 treatment of buds of the GA‐deficient gib‐1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression of both genes is regulated by gibberellins (GAs). Application of GA3 to developmentally arrested gib‐1 flower buds induced new expression of tgas100 mRNA 48 h post‐treatment, while an increased accumulation of tgas105 mRNA was found after 8 h. In situ analyses showed the spatial distribution of the expression of both genes within the tomato flower. One of the deduced polypeptides (TGAS105) displays similarities to cysteine‐rich extensin‐like proteins, while the other (TGAS100) shows significant homology with a stamen‐specific gene of Antirrhinum majus. Based on the deduced protein sequences, the possible function of the encoded proteins is discussed.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>Anthers</subject><subject>Antirrhinum majus</subject><subject>Biological and medical sciences</subject><subject>Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation</subject><subject>Blotting, Northern</subject><subject>Cell and Molecular Biology, Biochemistry and Molecular Physiology</subject><subject>Complementary DNA</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>Economic plant physiology</subject><subject>Extensin‐like</subject><subject>Flower buds</subject><subject>Flowering</subject><subject>Flowering, floral biology, reproduction patterns</subject><subject>flower‐specific gene expression</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>gib-1 gene</subject><subject>gibberellin</subject><subject>Gibberellins</subject><subject>Gibberellins - pharmacology</subject><subject>Growth and development</subject><subject>In Situ Hybridization</subject><subject>Lycopersicon esculentum</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Lycopersicon esculentum - growth & development</subject><subject>Messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Stems - genetics</subject><subject>Plant Stems - growth & development</subject><subject>Plants</subject><subject>Proteins</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Solanum lycopersicum</subject><subject>tgas100 gene</subject><subject>Tissue Distribution</subject><subject>Vegetative and sexual reproduction, floral biology, fructification</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxSMEotvCnQvIFyo4ZGtn4jg5ovKnoAoEBQn1YtnOeOUliYOd0N0bH4HPyCchq0TtkdNo9H7vaTQvSZ4wuma0grMt7rQfzjisoSjWnN1LViwvaJrlwO4nK0qzLKUVF0fJcYxbSimnnD9MjhgTOaM5WyX-C27GRg3Od8Rbgrs-YIzLNtx40vlf2BDb-BsMf3__iT0aZ50hG-wwEht8SwbfqsGTF1e-Ud3YkmZvfI8hOjO2L4nek43TGgM2jeseJQ-saiI-XuZJ8u3tm6_nF-nlp3fvz19dpoZn-ZBCbTRoUdhMAJQZGM0LxnKESoMQFhC4FcaiRgq8pkIhF2VdUmtqarjO4CQ5nXP74H-OGAfZumimE1SHfoxSMABelfl_QVZmPK_YAaQzaIKPMaCVfXCtCnvJqDy0Iec2JAc5tSE5myzPluxRt1jfGZb3T8DzBVDRqMYG1RkX7zjgwCpeTNzTmdvGwYdbPYOiEllx0NNZd3HA3a2uwg9ZCBBcXny_luJz8frq-sPHKfYf1a2vuw</recordid><startdate>20020101</startdate><enddate>20020101</enddate><creator>Van den Heuvel, Koen J.</creator><creator>Van Lipzig, Rosalinde H.</creator><creator>Barendse, Gerard W.</creator><creator>Wullems, George J.</creator><general>Oxford University Press</general><general>OXFORD UNIVERSITY PRESS</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020101</creationdate><title>Regulation of expression of two novel flower‐specific genes from tomato (Solanum lycopersicum) by gibberellin</title><author>Van den Heuvel, Koen J. ; Van Lipzig, Rosalinde H. ; Barendse, Gerard W. ; Wullems, George J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-3dcb3b76f2733823cb56114e39b377f3e35f7cfebe035d07ae578d80fcd0c5b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>Anthers</topic><topic>Antirrhinum majus</topic><topic>Biological and medical sciences</topic><topic>Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation</topic><topic>Blotting, Northern</topic><topic>Cell and Molecular Biology, Biochemistry and Molecular Physiology</topic><topic>Complementary DNA</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Economic plant physiology</topic><topic>Extensin‐like</topic><topic>Flower buds</topic><topic>Flowering</topic><topic>Flowering, floral biology, reproduction patterns</topic><topic>flower‐specific gene expression</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>gib-1 gene</topic><topic>gibberellin</topic><topic>Gibberellins</topic><topic>Gibberellins - pharmacology</topic><topic>Growth and development</topic><topic>In Situ Hybridization</topic><topic>Lycopersicon esculentum</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Lycopersicon esculentum - growth & development</topic><topic>Messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Stems - genetics</topic><topic>Plant Stems - growth & development</topic><topic>Plants</topic><topic>Proteins</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Solanum lycopersicum</topic><topic>tgas100 gene</topic><topic>Tissue Distribution</topic><topic>Vegetative and sexual reproduction, floral biology, fructification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van den Heuvel, Koen J.</creatorcontrib><creatorcontrib>Van Lipzig, Rosalinde H.</creatorcontrib><creatorcontrib>Barendse, Gerard W.</creatorcontrib><creatorcontrib>Wullems, George J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van den Heuvel, Koen J.</au><au>Van Lipzig, Rosalinde H.</au><au>Barendse, Gerard W.</au><au>Wullems, George J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of expression of two novel flower‐specific genes from tomato (Solanum lycopersicum) by gibberellin</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J. Exp. Bot</addtitle><date>2002-01-01</date><risdate>2002</risdate><volume>53</volume><issue>366</issue><spage>51</spage><epage>59</epage><pages>51-59</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>Two flower‐specific cDNAs have been isolated after differential screening of an anther cDNA library. This library was constructed 48 h after GA3 treatment of buds of the GA‐deficient gib‐1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression of both genes is regulated by gibberellins (GAs). Application of GA3 to developmentally arrested gib‐1 flower buds induced new expression of tgas100 mRNA 48 h post‐treatment, while an increased accumulation of tgas105 mRNA was found after 8 h. In situ analyses showed the spatial distribution of the expression of both genes within the tomato flower. One of the deduced polypeptides (TGAS105) displays similarities to cysteine‐rich extensin‐like proteins, while the other (TGAS100) shows significant homology with a stamen‐specific gene of Antirrhinum majus. Based on the deduced protein sequences, the possible function of the encoded proteins is discussed.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11741041</pmid><doi>10.1093/jexbot/53.366.51</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agronomy. Soil science and plant productions Amino Acid Sequence Anthers Antirrhinum majus Biological and medical sciences Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation Blotting, Northern Cell and Molecular Biology, Biochemistry and Molecular Physiology Complementary DNA DNA, Complementary - chemistry DNA, Complementary - genetics Economic plant physiology Extensin‐like Flower buds Flowering Flowering, floral biology, reproduction patterns flower‐specific gene expression Fundamental and applied biological sciences. Psychology Gene expression regulation Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Plant - drug effects gib-1 gene gibberellin Gibberellins Gibberellins - pharmacology Growth and development In Situ Hybridization Lycopersicon esculentum Lycopersicon esculentum - genetics Lycopersicon esculentum - growth & development Messenger RNA Molecular Sequence Data Mutation Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Plant Stems - genetics Plant Stems - growth & development Plants Proteins RNA RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Solanum lycopersicum tgas100 gene Tissue Distribution Vegetative and sexual reproduction, floral biology, fructification |
| title | Regulation of expression of two novel flower‐specific genes from tomato (Solanum lycopersicum) by gibberellin |
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