Anti-inflammatory effects of intramammary infusions of glycyrrhizin in lactating cows with mastitis caused by coagulase-negative staphylococci
To determine the anti-inflammatory effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with coagulase-negative staphylococci (CNS). 12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis....
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| Veröffentlicht in: | American journal of veterinary research 2003-10, Vol.64 (10), p.1213-1220 |
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| container_title | American journal of veterinary research |
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| creator | Kai, K Komine, K.I Asai, K.I Kuroishi, T Komine, Y Kozutsumi, T Itagaki, M Ohta, M Endo, Y Kumagai, K |
| description | To determine the anti-inflammatory effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with coagulase-negative staphylococci (CNS).
12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis.
Clinical signs, number of bacteria in milk, somatic cell count (SCC) in milk, concentrations of alpha-lactalbumin and lactoferrin in milk, and concentration of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials (6 cows). Glands of 2 healthy cows were infused with staphylococcal enterotoxin; milk leukocytes were then harvested and incubated with various doses of GL.
In cows infected with CNS that had a low bacterial concentration in milk, infusion of GL alone resulted in significant improvements in swelling, firmness of glands, and number of clots in milk, and it decreased the SCC, but not significantly. Percentage of neutrophils decreased significantly (to < 30%) by 2 days after infusion. Use of lactoferrin as a marker of inflammation in mammary glands revealed a decrease in concentrations, whereas use of alpha-lactalbumin as a marker of recovery for mammary glands revealed significant increases in concentrations in the GL-infused group. Accompanying these anti-inflammatory effects, a decrease in the concentration of histamine in milk was observed in the GL-infused group. Glycyrrhizin decreased histamine production by milk leukocytes in a concentration-dependent manner.
Infusion of GL may regulate intramammary inflammation through modulation of inflammatory mediators such as histamine. |
| doi_str_mv | 10.2460/ajvr.2003.64.1213 |
| format | Article |
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12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis.
Clinical signs, number of bacteria in milk, somatic cell count (SCC) in milk, concentrations of alpha-lactalbumin and lactoferrin in milk, and concentration of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials (6 cows). Glands of 2 healthy cows were infused with staphylococcal enterotoxin; milk leukocytes were then harvested and incubated with various doses of GL.
In cows infected with CNS that had a low bacterial concentration in milk, infusion of GL alone resulted in significant improvements in swelling, firmness of glands, and number of clots in milk, and it decreased the SCC, but not significantly. Percentage of neutrophils decreased significantly (to < 30%) by 2 days after infusion. Use of lactoferrin as a marker of inflammation in mammary glands revealed a decrease in concentrations, whereas use of alpha-lactalbumin as a marker of recovery for mammary glands revealed significant increases in concentrations in the GL-infused group. Accompanying these anti-inflammatory effects, a decrease in the concentration of histamine in milk was observed in the GL-infused group. Glycyrrhizin decreased histamine production by milk leukocytes in a concentration-dependent manner.
Infusion of GL may regulate intramammary inflammation through modulation of inflammatory mediators such as histamine.</description><identifier>ISSN: 0002-9645</identifier><identifier>EISSN: 1943-5681</identifier><identifier>DOI: 10.2460/ajvr.2003.64.1213</identifier><identifier>PMID: 14596456</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; anti-inflammatory activity ; Anti-Inflammatory Agents, Non-Steroidal - administration & dosage ; Anti-Inflammatory Agents, Non-Steroidal - therapeutic use ; antimicrobial agents ; bacterial contamination ; bacterial toxins ; bovine mastitis ; Cattle ; coagulase negative staphylococci ; dairy cows ; dosage ; drug evaluation ; drug therapy ; enterotoxins ; Female ; food contamination ; Glycyrrhizic Acid - administration & dosage ; Glycyrrhizic Acid - therapeutic use ; glycyrrhizin ; histamine ; Histamine - metabolism ; Holstein ; Infusions, Parenteral ; lactalbumin ; Lactalbumin - metabolism ; lactoferrin ; Lactoferrin - metabolism ; Leukocytes - drug effects ; Leukocytes - physiology ; mammary glands ; Mammary Glands, Animal ; Mastitis, Bovine - drug therapy ; Mastitis, Bovine - microbiology ; milk ; Milk - chemistry ; Milk - cytology ; Milk - microbiology ; neutrophils ; somatic cell count ; Staphylococcal Infections - drug therapy ; Staphylococcal Infections - veterinary ; swelling (physiological) ; Time Factors</subject><ispartof>American journal of veterinary research, 2003-10, Vol.64 (10), p.1213-1220</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-d2c850055c2ac1766f3cf8fe62098ba3543ec8eb26be9df4f7cb79e677c6f2863</citedby><cites>FETCH-LOGICAL-c366t-d2c850055c2ac1766f3cf8fe62098ba3543ec8eb26be9df4f7cb79e677c6f2863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14596456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kai, K</creatorcontrib><creatorcontrib>Komine, K.I</creatorcontrib><creatorcontrib>Asai, K.I</creatorcontrib><creatorcontrib>Kuroishi, T</creatorcontrib><creatorcontrib>Komine, Y</creatorcontrib><creatorcontrib>Kozutsumi, T</creatorcontrib><creatorcontrib>Itagaki, M</creatorcontrib><creatorcontrib>Ohta, M</creatorcontrib><creatorcontrib>Endo, Y</creatorcontrib><creatorcontrib>Kumagai, K</creatorcontrib><title>Anti-inflammatory effects of intramammary infusions of glycyrrhizin in lactating cows with mastitis caused by coagulase-negative staphylococci</title><title>American journal of veterinary research</title><addtitle>Am J Vet Res</addtitle><description>To determine the anti-inflammatory effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with coagulase-negative staphylococci (CNS).
12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis.
Clinical signs, number of bacteria in milk, somatic cell count (SCC) in milk, concentrations of alpha-lactalbumin and lactoferrin in milk, and concentration of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials (6 cows). Glands of 2 healthy cows were infused with staphylococcal enterotoxin; milk leukocytes were then harvested and incubated with various doses of GL.
In cows infected with CNS that had a low bacterial concentration in milk, infusion of GL alone resulted in significant improvements in swelling, firmness of glands, and number of clots in milk, and it decreased the SCC, but not significantly. Percentage of neutrophils decreased significantly (to < 30%) by 2 days after infusion. Use of lactoferrin as a marker of inflammation in mammary glands revealed a decrease in concentrations, whereas use of alpha-lactalbumin as a marker of recovery for mammary glands revealed significant increases in concentrations in the GL-infused group. Accompanying these anti-inflammatory effects, a decrease in the concentration of histamine in milk was observed in the GL-infused group. Glycyrrhizin decreased histamine production by milk leukocytes in a concentration-dependent manner.
Infusion of GL may regulate intramammary inflammation through modulation of inflammatory mediators such as histamine.</description><subject>Animals</subject><subject>anti-inflammatory activity</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - administration & dosage</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - therapeutic use</subject><subject>antimicrobial agents</subject><subject>bacterial contamination</subject><subject>bacterial toxins</subject><subject>bovine mastitis</subject><subject>Cattle</subject><subject>coagulase negative staphylococci</subject><subject>dairy cows</subject><subject>dosage</subject><subject>drug evaluation</subject><subject>drug therapy</subject><subject>enterotoxins</subject><subject>Female</subject><subject>food contamination</subject><subject>Glycyrrhizic Acid - administration & dosage</subject><subject>Glycyrrhizic Acid - therapeutic use</subject><subject>glycyrrhizin</subject><subject>histamine</subject><subject>Histamine - metabolism</subject><subject>Holstein</subject><subject>Infusions, Parenteral</subject><subject>lactalbumin</subject><subject>Lactalbumin - metabolism</subject><subject>lactoferrin</subject><subject>Lactoferrin - metabolism</subject><subject>Leukocytes - drug effects</subject><subject>Leukocytes - physiology</subject><subject>mammary glands</subject><subject>Mammary Glands, Animal</subject><subject>Mastitis, Bovine - drug therapy</subject><subject>Mastitis, Bovine - microbiology</subject><subject>milk</subject><subject>Milk - chemistry</subject><subject>Milk - cytology</subject><subject>Milk - microbiology</subject><subject>neutrophils</subject><subject>somatic cell count</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcal Infections - veterinary</subject><subject>swelling (physiological)</subject><subject>Time Factors</subject><issn>0002-9645</issn><issn>1943-5681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1u1DAUhS1URIfCA7BpvWKXwf9xllXVAlIlFtC15dyxM66SeGo7HYWH4JlJmJFYXemc75zFPQh9omTLhCJf7PNr2jJC-FaJLWWUv0Eb2gheSaXpBdoQQljVKCEv0fucnwmhTFP5Dl1SIVdZbdCf27GEKoy-t8NgS0wzdt47KBlHj8NYkh1WZ9EXaMohjv-crp9hTmkffodxcXBvodgSxg5DPGZ8DGWPB5tLKCFjsFN2O9zOi2m7qbfZVaPrFv7V4VzsYT_3ESJA-IDeettn9_F8r9DTw_2vu2_V44-v3-9uHyvgSpVqx0BLQqQEZoHWSnkOXnunGGl0a7kU3IF2LVOta3Ze-BraunGqrkF5phW_Qp9PvYcUXyaXixlCBtf3dnRxyqamnAumxQLSEwgp5pycN4cU1ncYSsw6gllHMOsIRgmzjrBkrs_lUzu43f_E-esLcHMCvI3Gdilk8_STEcoJaaTQUvO_I4aRqQ</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Kai, K</creator><creator>Komine, K.I</creator><creator>Asai, K.I</creator><creator>Kuroishi, T</creator><creator>Komine, Y</creator><creator>Kozutsumi, T</creator><creator>Itagaki, M</creator><creator>Ohta, M</creator><creator>Endo, Y</creator><creator>Kumagai, K</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Anti-inflammatory effects of intramammary infusions of glycyrrhizin in lactating cows with mastitis caused by coagulase-negative staphylococci</title><author>Kai, K ; Komine, K.I ; Asai, K.I ; Kuroishi, T ; Komine, Y ; Kozutsumi, T ; Itagaki, M ; Ohta, M ; Endo, Y ; Kumagai, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-d2c850055c2ac1766f3cf8fe62098ba3543ec8eb26be9df4f7cb79e677c6f2863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>anti-inflammatory activity</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - administration & dosage</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - therapeutic use</topic><topic>antimicrobial agents</topic><topic>bacterial contamination</topic><topic>bacterial toxins</topic><topic>bovine mastitis</topic><topic>Cattle</topic><topic>coagulase negative staphylococci</topic><topic>dairy cows</topic><topic>dosage</topic><topic>drug evaluation</topic><topic>drug therapy</topic><topic>enterotoxins</topic><topic>Female</topic><topic>food contamination</topic><topic>Glycyrrhizic Acid - administration & dosage</topic><topic>Glycyrrhizic Acid - therapeutic use</topic><topic>glycyrrhizin</topic><topic>histamine</topic><topic>Histamine - metabolism</topic><topic>Holstein</topic><topic>Infusions, Parenteral</topic><topic>lactalbumin</topic><topic>Lactalbumin - metabolism</topic><topic>lactoferrin</topic><topic>Lactoferrin - metabolism</topic><topic>Leukocytes - drug effects</topic><topic>Leukocytes - physiology</topic><topic>mammary glands</topic><topic>Mammary Glands, Animal</topic><topic>Mastitis, Bovine - drug therapy</topic><topic>Mastitis, Bovine - microbiology</topic><topic>milk</topic><topic>Milk - chemistry</topic><topic>Milk - cytology</topic><topic>Milk - microbiology</topic><topic>neutrophils</topic><topic>somatic cell count</topic><topic>Staphylococcal Infections - drug therapy</topic><topic>Staphylococcal Infections - veterinary</topic><topic>swelling (physiological)</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kai, K</creatorcontrib><creatorcontrib>Komine, K.I</creatorcontrib><creatorcontrib>Asai, K.I</creatorcontrib><creatorcontrib>Kuroishi, T</creatorcontrib><creatorcontrib>Komine, Y</creatorcontrib><creatorcontrib>Kozutsumi, T</creatorcontrib><creatorcontrib>Itagaki, M</creatorcontrib><creatorcontrib>Ohta, M</creatorcontrib><creatorcontrib>Endo, Y</creatorcontrib><creatorcontrib>Kumagai, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kai, K</au><au>Komine, K.I</au><au>Asai, K.I</au><au>Kuroishi, T</au><au>Komine, Y</au><au>Kozutsumi, T</au><au>Itagaki, M</au><au>Ohta, M</au><au>Endo, Y</au><au>Kumagai, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory effects of intramammary infusions of glycyrrhizin in lactating cows with mastitis caused by coagulase-negative staphylococci</atitle><jtitle>American journal of veterinary research</jtitle><addtitle>Am J Vet Res</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>64</volume><issue>10</issue><spage>1213</spage><epage>1220</epage><pages>1213-1220</pages><issn>0002-9645</issn><eissn>1943-5681</eissn><abstract>To determine the anti-inflammatory effects of glycyrrhizin (GL) in lactating cows with mastitis attributable to naturally occurring infection with coagulase-negative staphylococci (CNS).
12 lactating Holstein cows with mastitis attributable to infection with CNS and 2 healthy cows without mastitis.
Clinical signs, number of bacteria in milk, somatic cell count (SCC) in milk, concentrations of alpha-lactalbumin and lactoferrin in milk, and concentration of histamine in milk were investigated before and after intramammary infusion of GL (6 cows) or antimicrobials (6 cows). Glands of 2 healthy cows were infused with staphylococcal enterotoxin; milk leukocytes were then harvested and incubated with various doses of GL.
In cows infected with CNS that had a low bacterial concentration in milk, infusion of GL alone resulted in significant improvements in swelling, firmness of glands, and number of clots in milk, and it decreased the SCC, but not significantly. Percentage of neutrophils decreased significantly (to < 30%) by 2 days after infusion. Use of lactoferrin as a marker of inflammation in mammary glands revealed a decrease in concentrations, whereas use of alpha-lactalbumin as a marker of recovery for mammary glands revealed significant increases in concentrations in the GL-infused group. Accompanying these anti-inflammatory effects, a decrease in the concentration of histamine in milk was observed in the GL-infused group. Glycyrrhizin decreased histamine production by milk leukocytes in a concentration-dependent manner.
Infusion of GL may regulate intramammary inflammation through modulation of inflammatory mediators such as histamine.</abstract><cop>United States</cop><pmid>14596456</pmid><doi>10.2460/ajvr.2003.64.1213</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | American journal of veterinary research, 2003-10, Vol.64 (10), p.1213-1220 |
| issn | 0002-9645 1943-5681 |
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| subjects | Animals anti-inflammatory activity Anti-Inflammatory Agents, Non-Steroidal - administration & dosage Anti-Inflammatory Agents, Non-Steroidal - therapeutic use antimicrobial agents bacterial contamination bacterial toxins bovine mastitis Cattle coagulase negative staphylococci dairy cows dosage drug evaluation drug therapy enterotoxins Female food contamination Glycyrrhizic Acid - administration & dosage Glycyrrhizic Acid - therapeutic use glycyrrhizin histamine Histamine - metabolism Holstein Infusions, Parenteral lactalbumin Lactalbumin - metabolism lactoferrin Lactoferrin - metabolism Leukocytes - drug effects Leukocytes - physiology mammary glands Mammary Glands, Animal Mastitis, Bovine - drug therapy Mastitis, Bovine - microbiology milk Milk - chemistry Milk - cytology Milk - microbiology neutrophils somatic cell count Staphylococcal Infections - drug therapy Staphylococcal Infections - veterinary swelling (physiological) Time Factors |
| title | Anti-inflammatory effects of intramammary infusions of glycyrrhizin in lactating cows with mastitis caused by coagulase-negative staphylococci |
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