Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803
The iron deficiency-dependent regulation of isiAB transcription in Synechocystis sp. PCC 6803 was analyzed by fusion of modified isiAB promoter fragments to gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be show...
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| Veröffentlicht in: | FEMS microbiology letters 2003-10, Vol.227 (2), p.255-262 |
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| creator | Kunert, Anja Vinnemeier, Josef Erdmann, Norbert Hagemann, Martin |
| description | The iron deficiency-dependent regulation of
isiAB transcription in
Synechocystis sp. PCC 6803 was analyzed by fusion of modified
isiAB promoter fragments to
gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic
fur mutant a corresponding incomplete repression of
isiAB transcription under iron-replete conditions confirmed the role of Fur in
isiAB regulation. However, a 90 bp region upstream of the putative −35 box of the
isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism. |
| doi_str_mv | 10.1016/S0378-1097(03)00689-X |
| format | Article |
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isiAB transcription in
Synechocystis sp. PCC 6803 was analyzed by fusion of modified
isiAB promoter fragments to
gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic
fur mutant a corresponding incomplete repression of
isiAB transcription under iron-replete conditions confirmed the role of Fur in
isiAB regulation. However, a 90 bp region upstream of the putative −35 box of the
isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(03)00689-X</identifier><identifier>PMID: 14592717</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Bacteriology ; Biological and medical sciences ; Cyanobacteria - drug effects ; Cyanobacteria - genetics ; Cyanobacteria - metabolism ; Cyanophyta ; Flavodoxin - biosynthesis ; Flavodoxin - genetics ; Fundamental and applied biological sciences. Psychology ; Fur protein ; Gene Expression Regulation, Bacterial ; Genes, Reporter ; gfp assay ; Iron - pharmacology ; Iron regulation ; isiA gene ; isiAB ; isiB gene ; Light-Harvesting Protein Complexes - biosynthesis ; Light-Harvesting Protein Complexes - genetics ; Microbiology ; Miscellaneous ; Operon - drug effects ; Operon - genetics ; Repressor Proteins - genetics ; Repressor Proteins - physiology ; Synechocystis</subject><ispartof>FEMS microbiology letters, 2003-10, Vol.227 (2), p.255-262</ispartof><rights>2003 Federation of European Microbiological Societies</rights><rights>2003 Federation of European Microbiological Societies 2003</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5135-9c809bb42f9e09436f43e844f287cc53435fefa7059ebd3ce3b4bbb8e4eab3993</citedby><cites>FETCH-LOGICAL-c5135-9c809bb42f9e09436f43e844f287cc53435fefa7059ebd3ce3b4bbb8e4eab3993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0378-1097%2803%2900689-X$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0378-1097%2803%2900689-X$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15217389$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14592717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kunert, Anja</creatorcontrib><creatorcontrib>Vinnemeier, Josef</creatorcontrib><creatorcontrib>Erdmann, Norbert</creatorcontrib><creatorcontrib>Hagemann, Martin</creatorcontrib><title>Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>The iron deficiency-dependent regulation of
isiAB transcription in
Synechocystis sp. PCC 6803 was analyzed by fusion of modified
isiAB promoter fragments to
gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic
fur mutant a corresponding incomplete repression of
isiAB transcription under iron-replete conditions confirmed the role of Fur in
isiAB regulation. However, a 90 bp region upstream of the putative −35 box of the
isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism.</description><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cyanobacteria - drug effects</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - metabolism</subject><subject>Cyanophyta</subject><subject>Flavodoxin - biosynthesis</subject><subject>Flavodoxin - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fur protein</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Reporter</subject><subject>gfp assay</subject><subject>Iron - pharmacology</subject><subject>Iron regulation</subject><subject>isiA gene</subject><subject>isiAB</subject><subject>isiB gene</subject><subject>Light-Harvesting Protein Complexes - biosynthesis</subject><subject>Light-Harvesting Protein Complexes - genetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Operon - drug effects</subject><subject>Operon - genetics</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - physiology</subject><subject>Synechocystis</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd2K1DAYhoso7rh6CUpOFD3omjRJmxwt6-CoMKK4CnsWksxXN9ImNWmV3oGXbTozuAjCehSSPM_3w1sUjwk-I5jULy8xbURJsGyeY_oC41rI8upOsSK8YWUta3G3WP1BTooHKX3DGLMK1_eLE8K4rBrSrIpfn2CIkJILHpkZbaaIXEI-jGi8BtRr51EP9lp7l3pkgx9j6Drnv-6_XQy-dH43WWe6fE3u4hUKA-RnlMUFsbP2wWg7QnRTjy5nn6sFO6cxt0nDGfq4XqNaYPqwuNfqLsGj43lafNm8_rx-W24_vHm3vtiWlhPKS2kFlsawqpWAJaN1yygIxtpKNNZyyihvodUN5hLMjlqghhljBDDQhkpJT4tnh7pDDN8nSKPqXbLQddpDmJJqCK0EqcWtIGkqwTBnGeQH0MaQUoRWDdH1Os6KYLVkpfZZqSUIhanaZ6Wusvfk2GAyPexurGM4GXh6BHSyumuj9talG45XGRLLSvLA_XQdzP_XXW3ebyvOs4sPbpiGf5vlX2a5zH1-UCDH9MNBVMk68BZ2LoId1S64Wzb_DYXY0LQ</recordid><startdate>20031024</startdate><enddate>20031024</enddate><creator>Kunert, Anja</creator><creator>Vinnemeier, Josef</creator><creator>Erdmann, Norbert</creator><creator>Hagemann, Martin</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20031024</creationdate><title>Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803</title><author>Kunert, Anja ; Vinnemeier, Josef ; Erdmann, Norbert ; Hagemann, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5135-9c809bb42f9e09436f43e844f287cc53435fefa7059ebd3ce3b4bbb8e4eab3993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cyanobacteria - drug effects</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - metabolism</topic><topic>Cyanophyta</topic><topic>Flavodoxin - biosynthesis</topic><topic>Flavodoxin - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fur protein</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Reporter</topic><topic>gfp assay</topic><topic>Iron - pharmacology</topic><topic>Iron regulation</topic><topic>isiA gene</topic><topic>isiAB</topic><topic>isiB gene</topic><topic>Light-Harvesting Protein Complexes - biosynthesis</topic><topic>Light-Harvesting Protein Complexes - genetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Operon - drug effects</topic><topic>Operon - genetics</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - physiology</topic><topic>Synechocystis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kunert, Anja</creatorcontrib><creatorcontrib>Vinnemeier, Josef</creatorcontrib><creatorcontrib>Erdmann, Norbert</creatorcontrib><creatorcontrib>Hagemann, Martin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kunert, Anja</au><au>Vinnemeier, Josef</au><au>Erdmann, Norbert</au><au>Hagemann, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2003-10-24</date><risdate>2003</risdate><volume>227</volume><issue>2</issue><spage>255</spage><epage>262</epage><pages>255-262</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>The iron deficiency-dependent regulation of
isiAB transcription in
Synechocystis sp. PCC 6803 was analyzed by fusion of modified
isiAB promoter fragments to
gfp and in vivo quantification of Gfp fluorescence. For the putative Fur box only a slight repressing impact on promoter activity could be shown. In a heteroallelic
fur mutant a corresponding incomplete repression of
isiAB transcription under iron-replete conditions confirmed the role of Fur in
isiAB regulation. However, a 90 bp region upstream of the putative −35 box of the
isiAB promoter was essential for full promoter activity under iron-deplete conditions. This pattern indicates a dual promoter regulation by both a repressing mechanism exhibited via the Fur system and an unknown activating mechanism.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>14592717</pmid><doi>10.1016/S0378-1097(03)00689-X</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2003-10, Vol.227 (2), p.255-262 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71328168 |
| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacterial Proteins - physiology Bacteriology Biological and medical sciences Cyanobacteria - drug effects Cyanobacteria - genetics Cyanobacteria - metabolism Cyanophyta Flavodoxin - biosynthesis Flavodoxin - genetics Fundamental and applied biological sciences. Psychology Fur protein Gene Expression Regulation, Bacterial Genes, Reporter gfp assay Iron - pharmacology Iron regulation isiA gene isiAB isiB gene Light-Harvesting Protein Complexes - biosynthesis Light-Harvesting Protein Complexes - genetics Microbiology Miscellaneous Operon - drug effects Operon - genetics Repressor Proteins - genetics Repressor Proteins - physiology Synechocystis |
| title | Repression by Fur is not the main mechanism controlling the iron-inducible isiAB operon in the cyanobacterium Synechocystis sp. PCC 6803 |
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