Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss
A second TNF gene (TNF2) has been cloned and sequenced in rainbow trout. In common with the first TNF gene isolated (TNF1), this gene is more TNFα-like than TNFβ-like. The full length cDNA is 1519 bp, containing a 765 bp open reading frame. The gene has four exons, of 380, 49, 60 and 1030 bp, respec...
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| Veröffentlicht in: | Developmental and comparative immunology 2002-03, Vol.26 (2), p.161-172 |
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| container_title | Developmental and comparative immunology |
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| creator | Zou, J. Wang, T. Hirono, I. Aoki, T. Inagawa, H. Honda, T. Soma, G.-I. Ototake, M. Nakanishi, T. Ellis, A.E. Secombes, C.J. |
| description | A second TNF gene (TNF2) has been cloned and sequenced in rainbow trout. In common with the first TNF gene isolated (TNF1), this gene is more TNFα-like than TNFβ-like. The full length cDNA is 1519
bp, containing a 765
bp open reading frame. The gene has four exons, of 380, 49, 60 and 1030
bp, respectively. Analysis of the 5′ flanking regions of TNF1 and TNF2 reveals several interesting differences in identified transcriptional regulatory elements, with a CATAAA box present 26
bp upstream of the transcription start in both genes. Expression analysis in LPS stimulated macrophages has shown a much stronger expression of TNF2 relative to TNF1, with expression being detected earlier and lasting longer. |
| doi_str_mv | 10.1016/S0145-305X(01)00058-1 |
| format | Article |
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bp, containing a 765
bp open reading frame. The gene has four exons, of 380, 49, 60 and 1030
bp, respectively. Analysis of the 5′ flanking regions of TNF1 and TNF2 reveals several interesting differences in identified transcriptional regulatory elements, with a CATAAA box present 26
bp upstream of the transcription start in both genes. Expression analysis in LPS stimulated macrophages has shown a much stronger expression of TNF2 relative to TNF1, with expression being detected earlier and lasting longer.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/S0145-305X(01)00058-1</identifier><identifier>PMID: 11696381</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Southern - veterinary ; Brackish ; Cloning, Molecular ; Differential expression ; Freshwater ; Gene Expression Regulation - immunology ; Gene organisation ; Marine ; Molecular Sequence Data ; Oncorhynchus mykiss ; Oncorhynchus mykiss - genetics ; Oncorhynchus mykiss - immunology ; Rainbow trout ; Reverse Transcriptase Polymerase Chain Reaction - veterinary ; RNA - genetics ; RNA - metabolism ; Sequence Homology, Amino Acid ; TNF1 gene ; TNF2 gene ; TNFα ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumor Necrosis Factor-alpha - genetics</subject><ispartof>Developmental and comparative immunology, 2002-03, Vol.26 (2), p.161-172</ispartof><rights>2001 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-29c2b8f4cd18edc7de4fc70fd4a97235d51d2a51c095a27773e7bbf52840556a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0145305X01000581$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11696381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zou, J.</creatorcontrib><creatorcontrib>Wang, T.</creatorcontrib><creatorcontrib>Hirono, I.</creatorcontrib><creatorcontrib>Aoki, T.</creatorcontrib><creatorcontrib>Inagawa, H.</creatorcontrib><creatorcontrib>Honda, T.</creatorcontrib><creatorcontrib>Soma, G.-I.</creatorcontrib><creatorcontrib>Ototake, M.</creatorcontrib><creatorcontrib>Nakanishi, T.</creatorcontrib><creatorcontrib>Ellis, A.E.</creatorcontrib><creatorcontrib>Secombes, C.J.</creatorcontrib><title>Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>A second TNF gene (TNF2) has been cloned and sequenced in rainbow trout. In common with the first TNF gene isolated (TNF1), this gene is more TNFα-like than TNFβ-like. The full length cDNA is 1519
bp, containing a 765
bp open reading frame. The gene has four exons, of 380, 49, 60 and 1030
bp, respectively. Analysis of the 5′ flanking regions of TNF1 and TNF2 reveals several interesting differences in identified transcriptional regulatory elements, with a CATAAA box present 26
bp upstream of the transcription start in both genes. Expression analysis in LPS stimulated macrophages has shown a much stronger expression of TNF2 relative to TNF1, with expression being detected earlier and lasting longer.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Southern - veterinary</subject><subject>Brackish</subject><subject>Cloning, Molecular</subject><subject>Differential expression</subject><subject>Freshwater</subject><subject>Gene Expression Regulation - immunology</subject><subject>Gene organisation</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>Oncorhynchus mykiss - immunology</subject><subject>Rainbow trout</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - veterinary</subject><subject>RNA - genetics</subject><subject>RNA - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>TNF1 gene</subject><subject>TNF2 gene</subject><subject>TNFα</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1ERZfCRwD5hEAi4HHi2DkhVP60UqUeAImbcewxNWzsre207Lcn213BsafRSL_3RvMeIc-AvQEG_dsvDDrRtEx8f8ngFWNMqAYekBUoOTSMqeEhWf1DjsnjUn4tEFPAHpFjgH7oWwUr8uND8B4zxhrMmuKfTcZSQoo0eVpvE63zlDKNaHMqoVBvbF32nxix0BBpNiGO6ZbWnOb6ml5Gm_LVNtqrudBp-zuU8oQcebMu-PQwT8i3Tx-_np41F5efz0_fXzS2HXht-GD5qHxnHSh0VjrsvJXMu84MkrfCCXDcCLBsEIZLKVuU4-gFVx0TojftCXmx993kdD1jqXoKxeJ6bSKmuWgJLRewWN0HguJ91yuxgGIP7n4vGb3e5DCZvNXA9K4DfdeB3gWsGei7DjQsuueHA_M4ofuvOoS-AO_2AC553ATMutiA0aILGW3VLoV7TvwFY6eYGQ</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Zou, J.</creator><creator>Wang, T.</creator><creator>Hirono, I.</creator><creator>Aoki, T.</creator><creator>Inagawa, H.</creator><creator>Honda, T.</creator><creator>Soma, G.-I.</creator><creator>Ototake, M.</creator><creator>Nakanishi, T.</creator><creator>Ellis, A.E.</creator><creator>Secombes, C.J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020301</creationdate><title>Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss</title><author>Zou, J. ; Wang, T. ; Hirono, I. ; Aoki, T. ; Inagawa, H. ; Honda, T. ; Soma, G.-I. ; Ototake, M. ; Nakanishi, T. ; Ellis, A.E. ; Secombes, C.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-29c2b8f4cd18edc7de4fc70fd4a97235d51d2a51c095a27773e7bbf52840556a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Southern - veterinary</topic><topic>Brackish</topic><topic>Cloning, Molecular</topic><topic>Differential expression</topic><topic>Freshwater</topic><topic>Gene Expression Regulation - immunology</topic><topic>Gene organisation</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>Oncorhynchus mykiss - immunology</topic><topic>Rainbow trout</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - veterinary</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>TNF1 gene</topic><topic>TNF2 gene</topic><topic>TNFα</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zou, J.</creatorcontrib><creatorcontrib>Wang, T.</creatorcontrib><creatorcontrib>Hirono, I.</creatorcontrib><creatorcontrib>Aoki, T.</creatorcontrib><creatorcontrib>Inagawa, H.</creatorcontrib><creatorcontrib>Honda, T.</creatorcontrib><creatorcontrib>Soma, G.-I.</creatorcontrib><creatorcontrib>Ototake, M.</creatorcontrib><creatorcontrib>Nakanishi, T.</creatorcontrib><creatorcontrib>Ellis, A.E.</creatorcontrib><creatorcontrib>Secombes, C.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zou, J.</au><au>Wang, T.</au><au>Hirono, I.</au><au>Aoki, T.</au><au>Inagawa, H.</au><au>Honda, T.</au><au>Soma, G.-I.</au><au>Ototake, M.</au><au>Nakanishi, T.</au><au>Ellis, A.E.</au><au>Secombes, C.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>26</volume><issue>2</issue><spage>161</spage><epage>172</epage><pages>161-172</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>A second TNF gene (TNF2) has been cloned and sequenced in rainbow trout. In common with the first TNF gene isolated (TNF1), this gene is more TNFα-like than TNFβ-like. The full length cDNA is 1519
bp, containing a 765
bp open reading frame. The gene has four exons, of 380, 49, 60 and 1030
bp, respectively. Analysis of the 5′ flanking regions of TNF1 and TNF2 reveals several interesting differences in identified transcriptional regulatory elements, with a CATAAA box present 26
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| subjects | Amino Acid Sequence Animals Base Sequence Blotting, Southern - veterinary Brackish Cloning, Molecular Differential expression Freshwater Gene Expression Regulation - immunology Gene organisation Marine Molecular Sequence Data Oncorhynchus mykiss Oncorhynchus mykiss - genetics Oncorhynchus mykiss - immunology Rainbow trout Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA - genetics RNA - metabolism Sequence Homology, Amino Acid TNF1 gene TNF2 gene TNFα Tumor Necrosis Factor-alpha - biosynthesis Tumor Necrosis Factor-alpha - genetics |
| title | Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss |
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