Retention of virulence in viable but non-culturable halophilic Vibrio spp

The viable but non-culturable (VBNC) forms of two environmental strains of Vibrio alginolyticus 1 and Vibrio parahaemolyticus 66 and one strain of V. parahaemolyticus ATCC 43996 showing virulence characteristics (hemolysin production, adhesive and/or cytotoxic ability, in vivo enteropathogenicity) w...

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Veröffentlicht in:	International journal of food microbiology 2003-12, Vol.89 (1), p.31-39
Hauptverfasser:	Baffone, W., Citterio, B., Vittoria, E., Casaroli, A., Campana, R., Falzano, L., Donelli, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Colony Count, Microbial Culture Media - chemistry Environment Food industries Food microbiology Fundamental and applied biological sciences. Psychology Intestines - microbiology Male Mice Mice, Inbred BALB C Rats Retention Staining and Labeling VBNC Vibrio spp Vibrio - growth & development Vibrio - isolation & purification Vibrio - pathogenicity Vibrio alginolyticus Vibrio Infections - microbiology Vibrio parahaemolyticus Virulence
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Zusammenfassung:	The viable but non-culturable (VBNC) forms of two environmental strains of Vibrio alginolyticus 1 and Vibrio parahaemolyticus 66 and one strain of V. parahaemolyticus ATCC 43996 showing virulence characteristics (hemolysin production, adhesive and/or cytotoxic ability, in vivo enteropathogenicity) were obtained by culturing bacteria in a microcosm consisting of artificial sea water (ASW) and incubating at 5 °C with shaking. Every 2 days, culturability of the cells in the microcosm was monitored by spread plates on BHI agar and total count and the percentage of viable cells were determined by double staining with DAPI and CTC. When cell growth was not detectable (
ISSN:	0168-1605 1879-3460
DOI:	10.1016/S0168-1605(03)00102-8