The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshift
We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of...
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| Veröffentlicht in: | Medical mycology (Oxford) 2001-10, Vol.39 (5), p.387-394 |
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| creator | GIL, M. L DELGADO, M. L GOZALBO, D |
| description | We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells. |
| doi_str_mv | 10.1080/714031054 |
| format | Article |
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L ; DELGADO, M. L ; GOZALBO, D</creator><creatorcontrib>GIL, M. L ; DELGADO, M. L ; GOZALBO, D</creatorcontrib><description>We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells.</description><identifier>ISSN: 1369-3786</identifier><identifier>EISSN: 1460-2709</identifier><identifier>DOI: 10.1080/714031054</identifier><identifier>PMID: 12054048</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antifungal agents ; Biological and medical sciences ; Candida albicans - enzymology ; Cell Wall - enzymology ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - analysis ; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism ; Medical sciences ; Microbiology ; Mycology ; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology ; Pharmacology. Drug treatments ; Temperature</subject><ispartof>Medical mycology (Oxford), 2001-10, Vol.39 (5), p.387-394</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c243t-17ff6bc7f4bbd06aba77f017f138197998408f07cf2a00eda166f9b8990177f43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14166472$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12054048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GIL, M. L</creatorcontrib><creatorcontrib>DELGADO, M. L</creatorcontrib><creatorcontrib>GOZALBO, D</creatorcontrib><title>The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshift</title><title>Medical mycology (Oxford)</title><addtitle>Med Mycol</addtitle><description>We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells.</description><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antifungal agents</subject><subject>Biological and medical sciences</subject><subject>Candida albicans - enzymology</subject><subject>Cell Wall - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - analysis</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Temperature</subject><issn>1369-3786</issn><issn>1460-2709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkctu1TAQhi1ERUthwQsgb0BiEbBjN46X6IibVIlNu44mzrgx8omDx2l1nqSvW5ce0dWMZr7558bYOyk-S9GLL0ZqoaS40C_YmdSdaFoj7Mvqq842yvTdKXtN9EcIaWyrXrFT2VZY6P6M3V_NyHewTGECDnEMDhbiDmPkdxBjA0TJBSg48Zt4cJghTjgfJmxUs86J1rnm-L9QTje4ACEHV8JtKAceFpexRqh6PCOtaanpkjgVyLdQQlp4bc0L7teqXLaMfFtpDr68YSceIuHboz1n19-_Xe1-Npe_f_zafb1sXKtVaaTxvhud8XocJ9HBCMb4uqaXqpfWWNtr0XthnG9BCJxAdp23Y29thWqVOmcfn3TXnP5uSGXYB3pcHxZMGw1GKmEurKrgpyfQ5USU0Q9rDnvIh0GK4fELw_8vVPb9UXQb9zg9k8ezV-DDEQByEH2GxQV65nQdU5tWPQDJ5JHq</recordid><startdate>20011001</startdate><enddate>20011001</enddate><creator>GIL, M. L</creator><creator>DELGADO, M. 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Antiparasitic agents</topic><topic>Antifungal agents</topic><topic>Biological and medical sciences</topic><topic>Candida albicans - enzymology</topic><topic>Cell Wall - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - analysis</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GIL, M. L</creatorcontrib><creatorcontrib>DELGADO, M. L</creatorcontrib><creatorcontrib>GOZALBO, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Medical mycology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GIL, M. L</au><au>DELGADO, M. 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This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. albicans cells.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><pmid>12054048</pmid><doi>10.1080/714031054</doi><tpages>8</tpages></addata></record> |
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| ispartof | Medical mycology (Oxford), 2001-10, Vol.39 (5), p.387-394 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Taylor & Francis Journals Complete |
| subjects | Antibiotics. Antiinfectious agents. Antiparasitic agents Antifungal agents Biological and medical sciences Candida albicans - enzymology Cell Wall - enzymology Fundamental and applied biological sciences. Psychology Fungal Proteins - analysis Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism Medical sciences Microbiology Mycology Pathogenicity, host-agent relations, miscellaneous strains, epidemiology Pharmacology. Drug treatments Temperature |
| title | The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshift |
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