Selection of hapten-specific single-domain antibodies from a non-immunized llama ribosome display library

Picloram-specific variable fragments (V HHs) of heavy chain antibodies (HCAbs) were selected from a naı̈ve-llama library using ribosome display technology. A cDNA library of V HHs was constructed from lymphocytes of a non-immunized llama and engineered to allow in vitro transcription and translation...

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Veröffentlicht in:	Journal of immunological methods 2003-10, Vol.281 (1), p.161-175
Hauptverfasser:	Yau, Kerrm Y.F, Groves, Maria A.T, Li, Shenghua, Sheedy, Claudia, Lee, Hung, Tanha, Jamshid, MacKenzie, C.Roger, Jermutus, Lutz, Hall, J.Christopher
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity maturation Amino Acid Sequence Animals Antibody Affinity Biological and medical sciences Camelids, New World - immunology Complementarity Determining Regions Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Library Hapten Haptens - immunology Immunization Immunoglobulin Heavy Chains - immunology Immunoglobulin Variable Region - immunology Lama glama Molecular immunology Molecular Sequence Data Naı̈ve llama library Peptide Library Picloram - immunology Ribosome display Ribosomes - metabolism Single-domain antibody Surface Plasmon Resonance Techniques
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