Effects of Maturase Binding and Mg2+ Concentration on Group II Intron RNA Folding Investigated by UV Cross-Linking

The Lactococcus lactis Ll.LtrB group II intron encodes a reverse transcriptase/maturase (LtrA protein) that promotes RNA splicing by stabilizing the catalytically active RNA structure. Here, we mapped 17 UV cross-links induced in both wild-type Ll.LtrB RNA and Ll.LtrB-Δ2486 RNA, which has a branch-p...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemistry (Easton) 2003-11, Vol.42 (43), p.12466-12480
Hauptverfasser:	Noah, James W, Lambowitz, Alan M
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA Primers Introns Kinetics Magnesium - metabolism Molecular Sequence Data Nucleic Acid Conformation Protein Binding Protein Structure, Tertiary RNA Splicing RNA, Bacterial - chemistry RNA, Bacterial - metabolism RNA-Directed DNA Polymerase - metabolism Ultraviolet Rays
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!