cDNA nucleotide sequence coding for stearoyl-CoA desaturase and its expression in the zebrafish (Danio rerio) embryo
A cDNA sequence of stearoyl‐CoA desaturase (SCD) was determined from zebrafish (Danio rerio) and compared to the corresponding genes in several teleosts. Zebrafish SCD cDNA has a size of 1,061 bp, encodes a polypeptide of 325 amino acids, and shares 88, 85, 84, and 83% similarities with tilapia (Ore...
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| Veröffentlicht in: | Molecular reproduction and development 2003-12, Vol.66 (4), p.325-333 |
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| creator | Hsieh, S.L. Liu, R.W. Wu, C.H. Cheng, W.T. Kuo, Ching-Ming |
| description | A cDNA sequence of stearoyl‐CoA desaturase (SCD) was determined from zebrafish (Danio rerio) and compared to the corresponding genes in several teleosts. Zebrafish SCD cDNA has a size of 1,061 bp, encodes a polypeptide of 325 amino acids, and shares 88, 85, 84, and 83% similarities with tilapia (Oreochromis mossambicus), grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio), and milkfish (Chanos chanos), respectively. This 1,061 bp sequence specifies a protein that, in common with other fatty acid desaturases, contains three histidine boxes, believed to be involved in catalysis. These observations suggested that SCD genes are highly conserved. In addition, an oligonucleotide probe complementary to zebrafish SCD mRNA was hybridized to mRNA of approximately 396 bases with Northern blot analysis. The Northern blot and RT‐PCR analyses showed that the SCD mRNA was expressed predominantly in the liver, intestine, gill, and muscle, while a lower level was found in the brain. Furthermore, we utilized whole‐mount in situ hybridization and real‐time quantitative RT‐PCR to identify expression of the zebrafish SCD gene at five different stages of development. This revealed that very high levels of transcripts were found in zebrafish at all stages during embryogenesis and early development. Mol. Reprod. Dev. 66: 325–333, 2003. © 2003 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/mrd.10358 |
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Zebrafish SCD cDNA has a size of 1,061 bp, encodes a polypeptide of 325 amino acids, and shares 88, 85, 84, and 83% similarities with tilapia (Oreochromis mossambicus), grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio), and milkfish (Chanos chanos), respectively. This 1,061 bp sequence specifies a protein that, in common with other fatty acid desaturases, contains three histidine boxes, believed to be involved in catalysis. These observations suggested that SCD genes are highly conserved. In addition, an oligonucleotide probe complementary to zebrafish SCD mRNA was hybridized to mRNA of approximately 396 bases with Northern blot analysis. The Northern blot and RT‐PCR analyses showed that the SCD mRNA was expressed predominantly in the liver, intestine, gill, and muscle, while a lower level was found in the brain. Furthermore, we utilized whole‐mount in situ hybridization and real‐time quantitative RT‐PCR to identify expression of the zebrafish SCD gene at five different stages of development. This revealed that very high levels of transcripts were found in zebrafish at all stages during embryogenesis and early development. Mol. Reprod. Dev. 66: 325–333, 2003. © 2003 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.10358</identifier><identifier>PMID: 14579408</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cell metabolism, cell oxidation ; Cell physiology ; Chanos chanos ; Cloning, Molecular ; Ctenopharyngodon idella ; Cyprinus carpio ; Danio rerio ; development ; DNA, Complementary - genetics ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Humans ; In Situ Hybridization ; Molecular and cellular biology ; Molecular Sequence Data ; Northern blot analysis ; Oreochromis mossambicus ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Alignment ; Stearoyl-CoA Desaturase - chemistry ; Stearoyl-CoA Desaturase - genetics ; Teleostei ; teleosts ; Zebrafish - embryology ; Zebrafish - genetics</subject><ispartof>Molecular reproduction and development, 2003-12, Vol.66 (4), p.325-333</ispartof><rights>Copyright © 2003 Wiley‐Liss, Inc.</rights><rights>Copyright 2003 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4208-9e15f75bc447ed22f7fc5224955c9239ca411517d0056183682d8badaedb58ff3</citedby><cites>FETCH-LOGICAL-c4208-9e15f75bc447ed22f7fc5224955c9239ca411517d0056183682d8badaedb58ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.10358$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.10358$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15491854$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14579408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsieh, S.L.</creatorcontrib><creatorcontrib>Liu, R.W.</creatorcontrib><creatorcontrib>Wu, C.H.</creatorcontrib><creatorcontrib>Cheng, W.T.</creatorcontrib><creatorcontrib>Kuo, Ching-Ming</creatorcontrib><title>cDNA nucleotide sequence coding for stearoyl-CoA desaturase and its expression in the zebrafish (Danio rerio) embryo</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>A cDNA sequence of stearoyl‐CoA desaturase (SCD) was determined from zebrafish (Danio rerio) and compared to the corresponding genes in several teleosts. Zebrafish SCD cDNA has a size of 1,061 bp, encodes a polypeptide of 325 amino acids, and shares 88, 85, 84, and 83% similarities with tilapia (Oreochromis mossambicus), grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio), and milkfish (Chanos chanos), respectively. This 1,061 bp sequence specifies a protein that, in common with other fatty acid desaturases, contains three histidine boxes, believed to be involved in catalysis. These observations suggested that SCD genes are highly conserved. In addition, an oligonucleotide probe complementary to zebrafish SCD mRNA was hybridized to mRNA of approximately 396 bases with Northern blot analysis. The Northern blot and RT‐PCR analyses showed that the SCD mRNA was expressed predominantly in the liver, intestine, gill, and muscle, while a lower level was found in the brain. Furthermore, we utilized whole‐mount in situ hybridization and real‐time quantitative RT‐PCR to identify expression of the zebrafish SCD gene at five different stages of development. This revealed that very high levels of transcripts were found in zebrafish at all stages during embryogenesis and early development. Mol. Reprod. Dev. 66: 325–333, 2003. © 2003 Wiley‐Liss, Inc.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell metabolism, cell oxidation</subject><subject>Cell physiology</subject><subject>Chanos chanos</subject><subject>Cloning, Molecular</subject><subject>Ctenopharyngodon idella</subject><subject>Cyprinus carpio</subject><subject>Danio rerio</subject><subject>development</subject><subject>DNA, Complementary - genetics</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Northern blot analysis</subject><subject>Oreochromis mossambicus</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Alignment</subject><subject>Stearoyl-CoA Desaturase - chemistry</subject><subject>Stearoyl-CoA Desaturase - genetics</subject><subject>Teleostei</subject><subject>teleosts</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UFvFCEUB_CJ0djaevALGC4aexgLDCxw3O7a2mRtk6qpN8LAw6IzsMJM7PrpnbpbezKeeIffey-8f1W9IPgtwZge99lNRcPlo2qfYCVrKhR_fFczXDNOv-xVz0r5hjFWSuKn1R5hXCiG5X412OXFHMXRdpCG4AAV-DFCtIBsciF-RT5lVAYwOW26epHmyEExw5hNAWSiQ2EoCG7XGUoJKaIQ0XAD6Be02fhQbtCbpYkhoQw5pCMEfZs36bB64k1X4PnuPag-n777tHhfry7PzhfzVW0ZxbJWQLgXvLWMCXCUeuEtp5Qpzq2ijbKGEcKJcBjzGZHNTFInW-MMuJZL75uD6vV27jqn6Vdl0H0oFrrOREhj0YJQxRVR_4VEUIUVFRM82kKbUykZvF7n0Ju80QTruyz0lIX-k8VkX-6Gjm0P7kHujj-BVztgijWdzybaUB4cZ4pIziZ3vHU_Qwebf2_UH66W96vrbUeYorv922Hydz0TjeD6-uJMn15fkdVHeqJXzW8UTq-X</recordid><startdate>200312</startdate><enddate>200312</enddate><creator>Hsieh, S.L.</creator><creator>Liu, R.W.</creator><creator>Wu, C.H.</creator><creator>Cheng, W.T.</creator><creator>Kuo, Ching-Ming</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>200312</creationdate><title>cDNA nucleotide sequence coding for stearoyl-CoA desaturase and its expression in the zebrafish (Danio rerio) embryo</title><author>Hsieh, S.L. ; Liu, R.W. ; Wu, C.H. ; Cheng, W.T. ; Kuo, Ching-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4208-9e15f75bc447ed22f7fc5224955c9239ca411517d0056183682d8badaedb58ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cell metabolism, cell oxidation</topic><topic>Cell physiology</topic><topic>Chanos chanos</topic><topic>Cloning, Molecular</topic><topic>Ctenopharyngodon idella</topic><topic>Cyprinus carpio</topic><topic>Danio rerio</topic><topic>development</topic><topic>DNA, Complementary - genetics</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Northern blot analysis</topic><topic>Oreochromis mossambicus</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Alignment</topic><topic>Stearoyl-CoA Desaturase - chemistry</topic><topic>Stearoyl-CoA Desaturase - genetics</topic><topic>Teleostei</topic><topic>teleosts</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsieh, S.L.</creatorcontrib><creatorcontrib>Liu, R.W.</creatorcontrib><creatorcontrib>Wu, C.H.</creatorcontrib><creatorcontrib>Cheng, W.T.</creatorcontrib><creatorcontrib>Kuo, Ching-Ming</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsieh, S.L.</au><au>Liu, R.W.</au><au>Wu, C.H.</au><au>Cheng, W.T.</au><au>Kuo, Ching-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA nucleotide sequence coding for stearoyl-CoA desaturase and its expression in the zebrafish (Danio rerio) embryo</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2003-12</date><risdate>2003</risdate><volume>66</volume><issue>4</issue><spage>325</spage><epage>333</epage><pages>325-333</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>A cDNA sequence of stearoyl‐CoA desaturase (SCD) was determined from zebrafish (Danio rerio) and compared to the corresponding genes in several teleosts. Zebrafish SCD cDNA has a size of 1,061 bp, encodes a polypeptide of 325 amino acids, and shares 88, 85, 84, and 83% similarities with tilapia (Oreochromis mossambicus), grass carp (Ctenopharyngodon idella), common carp (Cyprinus carpio), and milkfish (Chanos chanos), respectively. This 1,061 bp sequence specifies a protein that, in common with other fatty acid desaturases, contains three histidine boxes, believed to be involved in catalysis. These observations suggested that SCD genes are highly conserved. In addition, an oligonucleotide probe complementary to zebrafish SCD mRNA was hybridized to mRNA of approximately 396 bases with Northern blot analysis. The Northern blot and RT‐PCR analyses showed that the SCD mRNA was expressed predominantly in the liver, intestine, gill, and muscle, while a lower level was found in the brain. Furthermore, we utilized whole‐mount in situ hybridization and real‐time quantitative RT‐PCR to identify expression of the zebrafish SCD gene at five different stages of development. This revealed that very high levels of transcripts were found in zebrafish at all stages during embryogenesis and early development. Mol. Reprod. Dev. 66: 325–333, 2003. © 2003 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>14579408</pmid><doi>10.1002/mrd.10358</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern Cell metabolism, cell oxidation Cell physiology Chanos chanos Cloning, Molecular Ctenopharyngodon idella Cyprinus carpio Danio rerio development DNA, Complementary - genetics Freshwater Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Humans In Situ Hybridization Molecular and cellular biology Molecular Sequence Data Northern blot analysis Oreochromis mossambicus Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Alignment Stearoyl-CoA Desaturase - chemistry Stearoyl-CoA Desaturase - genetics Teleostei teleosts Zebrafish - embryology Zebrafish - genetics |
| title | cDNA nucleotide sequence coding for stearoyl-CoA desaturase and its expression in the zebrafish (Danio rerio) embryo |
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