Emx2 Promotes Symmetric Cell Divisions and a Multipotential Fate in Precursors from the Cerebral Cortex
Distinct sets of precursor cells generate the mammalian cerebral cortex. During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral...
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| Veröffentlicht in: | Molecular and cellular neuroscience 2001-11, Vol.18 (5), p.485-502 |
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| container_title | Molecular and cellular neuroscience |
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| creator | Heins, Nico Cremisi, Federico Malatesta, Paolo Gangemi, Rosaria M.R. Corte, Giorgio Price, Jack Goudreau, Guy Gruss, Peter Götz, Magdalena |
| description | Distinct sets of precursor cells generate the mammalian cerebral cortex. During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral transduction of the transcription factor Emx2 in precursors from the cerebral cortex results in a significant increase of large clones that are generated mostly by symmetric cell divisions and contain multiple cell types, comprising neurons and glial cells. Thus, Emx2 is the first cell-intrinsic determinant able to instruct CNS precursors towards a multipotential fate. To evaluate the role of endogenous Emx2 in cortical precursors, we examined cell division in Emx2−/− mice. These analyses further supported the role of endogenous Emx2 in the regulation of symmetric cell divisions in the developing cortex. |
| doi_str_mv | 10.1006/mcne.2001.1046 |
| format | Article |
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During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral transduction of the transcription factor Emx2 in precursors from the cerebral cortex results in a significant increase of large clones that are generated mostly by symmetric cell divisions and contain multiple cell types, comprising neurons and glial cells. Thus, Emx2 is the first cell-intrinsic determinant able to instruct CNS precursors towards a multipotential fate. To evaluate the role of endogenous Emx2 in cortical precursors, we examined cell division in Emx2−/− mice. These analyses further supported the role of endogenous Emx2 in the regulation of symmetric cell divisions in the developing cortex.</description><identifier>ISSN: 1044-7431</identifier><identifier>EISSN: 1095-9327</identifier><identifier>DOI: 10.1006/mcne.2001.1046</identifier><identifier>PMID: 11922140</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Body Patterning - genetics ; Cell Death - genetics ; Cell Differentiation - genetics ; Cell Division - genetics ; Cell Lineage - genetics ; Cell Movement - genetics ; Cerebral Cortex - cytology ; Cerebral Cortex - embryology ; Cerebral Cortex - metabolism ; Clone Cells - cytology ; Clone Cells - metabolism ; Female ; Fetus ; Genes, Reporter - physiology ; Genetic Vectors - physiology ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Male ; Mice ; Mice, Knockout ; Mitosis - physiology ; Neuroglia - cytology ; Neuroglia - metabolism ; Neurons - cytology ; Neurons - metabolism ; Stem Cells - cytology ; Stem Cells - metabolism ; Transcription Factors - deficiency ; Transcription Factors - genetics ; Transduction, Genetic</subject><ispartof>Molecular and cellular neuroscience, 2001-11, Vol.18 (5), p.485-502</ispartof><rights>2001 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-e494516f06c4ca13690285f2b556b876ac40dbbabd7efa4af742c182a346dd613</citedby><cites>FETCH-LOGICAL-c406t-e494516f06c4ca13690285f2b556b876ac40dbbabd7efa4af742c182a346dd613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mcne.2001.1046$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11922140$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heins, Nico</creatorcontrib><creatorcontrib>Cremisi, Federico</creatorcontrib><creatorcontrib>Malatesta, Paolo</creatorcontrib><creatorcontrib>Gangemi, Rosaria M.R.</creatorcontrib><creatorcontrib>Corte, Giorgio</creatorcontrib><creatorcontrib>Price, Jack</creatorcontrib><creatorcontrib>Goudreau, Guy</creatorcontrib><creatorcontrib>Gruss, Peter</creatorcontrib><creatorcontrib>Götz, Magdalena</creatorcontrib><title>Emx2 Promotes Symmetric Cell Divisions and a Multipotential Fate in Precursors from the Cerebral Cortex</title><title>Molecular and cellular neuroscience</title><addtitle>Mol Cell Neurosci</addtitle><description>Distinct sets of precursor cells generate the mammalian cerebral cortex. During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral transduction of the transcription factor Emx2 in precursors from the cerebral cortex results in a significant increase of large clones that are generated mostly by symmetric cell divisions and contain multiple cell types, comprising neurons and glial cells. Thus, Emx2 is the first cell-intrinsic determinant able to instruct CNS precursors towards a multipotential fate. To evaluate the role of endogenous Emx2 in cortical precursors, we examined cell division in Emx2−/− mice. These analyses further supported the role of endogenous Emx2 in the regulation of symmetric cell divisions in the developing cortex.</description><subject>Animals</subject><subject>Body Patterning - genetics</subject><subject>Cell Death - genetics</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Division - genetics</subject><subject>Cell Lineage - genetics</subject><subject>Cell Movement - genetics</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - embryology</subject><subject>Cerebral Cortex - metabolism</subject><subject>Clone Cells - cytology</subject><subject>Clone Cells - metabolism</subject><subject>Female</subject><subject>Fetus</subject><subject>Genes, Reporter - physiology</subject><subject>Genetic Vectors - physiology</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mitosis - physiology</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - metabolism</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - metabolism</subject><subject>Transcription Factors - deficiency</subject><subject>Transcription Factors - genetics</subject><subject>Transduction, Genetic</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFLwzAUh4Mobk6vHiUnb51JmqbtUeamgqKgnkOavmqkbWaSju2_N2UDT57ee_D9fvA-hC4pmVNCxE2ne5gzQmg8uThCU0rKLClTlh-PO-dJzlM6QWfefxNCMlamp2hCackY5WSKPpfdluFXZzsbwOO3XddBcEbjBbQtvjMb443tPVZ9jRV-Htpg1pHsg1EtXqkA2PQxDnpw3jqPm9iEwxfEvIPKRWhhXYDtOTppVOvh4jBn6GO1fF88JE8v94-L26dEcyJCArzkGRUNEZprRVNRElZkDauyTFRFLlTE6qpSVZ1Do7hqcs40LZhKuahrQdMZut73rp39GcAH2Rmv4y-qBzt4mVNWZkXKIjjfg9pZ7x00cu1Mp9xOUiJHtXJUK0e1clQbA1eH5qHqoP7DDy4jUOwBiP9tDDjptYFeQ22inyBra_7r_gXH8Ih-</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>Heins, Nico</creator><creator>Cremisi, Federico</creator><creator>Malatesta, Paolo</creator><creator>Gangemi, Rosaria M.R.</creator><creator>Corte, Giorgio</creator><creator>Price, Jack</creator><creator>Goudreau, Guy</creator><creator>Gruss, Peter</creator><creator>Götz, Magdalena</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20011101</creationdate><title>Emx2 Promotes Symmetric Cell Divisions and a Multipotential Fate in Precursors from the Cerebral Cortex</title><author>Heins, Nico ; Cremisi, Federico ; Malatesta, Paolo ; Gangemi, Rosaria M.R. ; Corte, Giorgio ; Price, Jack ; Goudreau, Guy ; Gruss, Peter ; Götz, Magdalena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-e494516f06c4ca13690285f2b556b876ac40dbbabd7efa4af742c182a346dd613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Body Patterning - genetics</topic><topic>Cell Death - genetics</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Division - genetics</topic><topic>Cell Lineage - genetics</topic><topic>Cell Movement - genetics</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - embryology</topic><topic>Cerebral Cortex - metabolism</topic><topic>Clone Cells - cytology</topic><topic>Clone Cells - metabolism</topic><topic>Female</topic><topic>Fetus</topic><topic>Genes, Reporter - physiology</topic><topic>Genetic Vectors - physiology</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Mitosis - physiology</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - metabolism</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - metabolism</topic><topic>Transcription Factors - deficiency</topic><topic>Transcription Factors - genetics</topic><topic>Transduction, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heins, Nico</creatorcontrib><creatorcontrib>Cremisi, Federico</creatorcontrib><creatorcontrib>Malatesta, Paolo</creatorcontrib><creatorcontrib>Gangemi, Rosaria M.R.</creatorcontrib><creatorcontrib>Corte, Giorgio</creatorcontrib><creatorcontrib>Price, Jack</creatorcontrib><creatorcontrib>Goudreau, Guy</creatorcontrib><creatorcontrib>Gruss, Peter</creatorcontrib><creatorcontrib>Götz, Magdalena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heins, Nico</au><au>Cremisi, Federico</au><au>Malatesta, Paolo</au><au>Gangemi, Rosaria M.R.</au><au>Corte, Giorgio</au><au>Price, Jack</au><au>Goudreau, Guy</au><au>Gruss, Peter</au><au>Götz, Magdalena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emx2 Promotes Symmetric Cell Divisions and a Multipotential Fate in Precursors from the Cerebral Cortex</atitle><jtitle>Molecular and cellular neuroscience</jtitle><addtitle>Mol Cell Neurosci</addtitle><date>2001-11-01</date><risdate>2001</risdate><volume>18</volume><issue>5</issue><spage>485</spage><epage>502</epage><pages>485-502</pages><issn>1044-7431</issn><eissn>1095-9327</eissn><abstract>Distinct sets of precursor cells generate the mammalian cerebral cortex. During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral transduction of the transcription factor Emx2 in precursors from the cerebral cortex results in a significant increase of large clones that are generated mostly by symmetric cell divisions and contain multiple cell types, comprising neurons and glial cells. Thus, Emx2 is the first cell-intrinsic determinant able to instruct CNS precursors towards a multipotential fate. To evaluate the role of endogenous Emx2 in cortical precursors, we examined cell division in Emx2−/− mice. These analyses further supported the role of endogenous Emx2 in the regulation of symmetric cell divisions in the developing cortex.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>11922140</pmid><doi>10.1006/mcne.2001.1046</doi><tpages>18</tpages></addata></record> |
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| subjects | Animals Body Patterning - genetics Cell Death - genetics Cell Differentiation - genetics Cell Division - genetics Cell Lineage - genetics Cell Movement - genetics Cerebral Cortex - cytology Cerebral Cortex - embryology Cerebral Cortex - metabolism Clone Cells - cytology Clone Cells - metabolism Female Fetus Genes, Reporter - physiology Genetic Vectors - physiology Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Male Mice Mice, Knockout Mitosis - physiology Neuroglia - cytology Neuroglia - metabolism Neurons - cytology Neurons - metabolism Stem Cells - cytology Stem Cells - metabolism Transcription Factors - deficiency Transcription Factors - genetics Transduction, Genetic |
| title | Emx2 Promotes Symmetric Cell Divisions and a Multipotential Fate in Precursors from the Cerebral Cortex |
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