Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti
Blood feeding is an essential developmental process for many arthropods and plays a significant role in disease transmission. Understanding physiological responses in the midgut is important because it is the primary site of blood meal digestion and pathogenic infection. Processes that occur in the...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2003-11, Vol.33 (11), p.1105-1122 |
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| creator | Sanders, Heather R. Evans, Amy M. Ross, Linda S. Gill, Sarjeet S. |
| description | Blood feeding is an essential developmental process for many arthropods and plays a significant role in disease transmission. Understanding physiological responses in the midgut is important because it is the primary site of blood meal digestion and pathogenic infection. Processes that occur in the midgut in response to a blood meal have been studied but are poorly understood. Here, we use cDNA microarrays to examine midgut gene expression on a global level in response to blood feeding to assist in unraveling these processes. We have developed
Aedes aegypti microarrays consisting of clones obtained from an expressed sequence tag project. Individual clones were amplified by polymerase chain reaction and printed onto glass slides. These microarrays were used to study the effects of a blood meal on midgut gene expression over a 72-h time course. As a result, a number of genes involved in processes such as nutrient uptake and metabolism, cellular stress responses, ion balance, and PM formation, as well as a number of unknown genes were induced or repressed in response to a blood meal based on this microarray data. |
| doi_str_mv | 10.1016/S0965-1748(03)00124-3 |
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Aedes aegypti microarrays consisting of clones obtained from an expressed sequence tag project. Individual clones were amplified by polymerase chain reaction and printed onto glass slides. These microarrays were used to study the effects of a blood meal on midgut gene expression over a 72-h time course. As a result, a number of genes involved in processes such as nutrient uptake and metabolism, cellular stress responses, ion balance, and PM formation, as well as a number of unknown genes were induced or repressed in response to a blood meal based on this microarray data.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/S0965-1748(03)00124-3</identifier><identifier>PMID: 14563362</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aedes - genetics ; Aedes - metabolism ; Aedes - physiology ; Aedes aegypti ; Animals ; arthropods ; Biological Transport - genetics ; blood ; Blood - metabolism ; Blood meal ; clones ; complementary DNA ; digestion ; Digestive System - metabolism ; disease transmission ; disease vectors ; DNA Primers - genetics ; Expressed Sequence Tags ; Fatty Acids - metabolism ; Feeding Behavior ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation - physiology ; genes ; hematophagy ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; metabolism ; microarray technology ; Midgut ; nutrient uptake ; Oligonucleotide Array Sequence Analysis ; Osmotic Pressure ; Oxidative Stress - genetics ; polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Signal Transduction ; stress response ; Time Factors ; Transcription, Genetic</subject><ispartof>Insect biochemistry and molecular biology, 2003-11, Vol.33 (11), p.1105-1122</ispartof><rights>2003 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-c2259d8776ab5872582c18a958161309dc3d33622c9ecf1649099630f45f05fd3</citedby><cites>FETCH-LOGICAL-c383t-c2259d8776ab5872582c18a958161309dc3d33622c9ecf1649099630f45f05fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0965-1748(03)00124-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14563362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanders, Heather R.</creatorcontrib><creatorcontrib>Evans, Amy M.</creatorcontrib><creatorcontrib>Ross, Linda S.</creatorcontrib><creatorcontrib>Gill, Sarjeet S.</creatorcontrib><title>Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>Blood feeding is an essential developmental process for many arthropods and plays a significant role in disease transmission. Understanding physiological responses in the midgut is important because it is the primary site of blood meal digestion and pathogenic infection. Processes that occur in the midgut in response to a blood meal have been studied but are poorly understood. Here, we use cDNA microarrays to examine midgut gene expression on a global level in response to blood feeding to assist in unraveling these processes. We have developed
Aedes aegypti microarrays consisting of clones obtained from an expressed sequence tag project. Individual clones were amplified by polymerase chain reaction and printed onto glass slides. These microarrays were used to study the effects of a blood meal on midgut gene expression over a 72-h time course. As a result, a number of genes involved in processes such as nutrient uptake and metabolism, cellular stress responses, ion balance, and PM formation, as well as a number of unknown genes were induced or repressed in response to a blood meal based on this microarray data.</description><subject>Aedes - genetics</subject><subject>Aedes - metabolism</subject><subject>Aedes - physiology</subject><subject>Aedes aegypti</subject><subject>Animals</subject><subject>arthropods</subject><subject>Biological Transport - genetics</subject><subject>blood</subject><subject>Blood - metabolism</subject><subject>Blood meal</subject><subject>clones</subject><subject>complementary DNA</subject><subject>digestion</subject><subject>Digestive System - metabolism</subject><subject>disease transmission</subject><subject>disease vectors</subject><subject>DNA Primers - genetics</subject><subject>Expressed Sequence Tags</subject><subject>Fatty Acids - metabolism</subject><subject>Feeding Behavior</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - physiology</subject><subject>genes</subject><subject>hematophagy</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>metabolism</subject><subject>microarray technology</subject><subject>Midgut</subject><subject>nutrient uptake</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Osmotic Pressure</subject><subject>Oxidative Stress - genetics</subject><subject>polymerase chain reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>Signal Transduction</subject><subject>stress response</subject><subject>Time Factors</subject><subject>Transcription, Genetic</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1ERZfCRwByQiARGNvxvxMqFVCkShxKz8ZrT1KjJF7spGq_PUl3RY89jUbzezOj9wh5ReEjBSo_XYKRoqaq0e-AvwegrKn5E7KhWpkaWANPyeY_ckyel_IHAJpGqGfkmDZCci7Zhvz-0qcUqgFdX8UxzB5L1fVpu7T-2o3d0saxGmLo5qnqcMQKb3cZS4lpXCfTNVYhFnQFqxv0U8ofqlMMi8xhd7eb4gty1Lq-4MtDPSFX377-OjuvL35-_3F2elF7rvlUe8aECVop6bZCKyY081Q7IzSVlIMJnof1Y-YN-pbKxoAxkkPbiBZEG_gJebvfu8vp74xlskMsHvvejZjmYhVlhoKWj4JUa9MwrRZQ7EGfUykZW7vLcXD5zlKwawb2PgO7GmyB2_sMLF90rw8H5u2A4UF1MH0B3uyB1iXruhyLvbpkQAUAAynVuuLznsDFsZuI2RYfcfQYYl5MtiHFR574B1Pmnfc</recordid><startdate>200311</startdate><enddate>200311</enddate><creator>Sanders, Heather R.</creator><creator>Evans, Amy M.</creator><creator>Ross, Linda S.</creator><creator>Gill, Sarjeet S.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>200311</creationdate><title>Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti</title><author>Sanders, Heather R. ; Evans, Amy M. ; Ross, Linda S. ; Gill, Sarjeet S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-c2259d8776ab5872582c18a958161309dc3d33622c9ecf1649099630f45f05fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aedes - genetics</topic><topic>Aedes - metabolism</topic><topic>Aedes - physiology</topic><topic>Aedes aegypti</topic><topic>Animals</topic><topic>arthropods</topic><topic>Biological Transport - genetics</topic><topic>blood</topic><topic>Blood - metabolism</topic><topic>Blood meal</topic><topic>clones</topic><topic>complementary DNA</topic><topic>digestion</topic><topic>Digestive System - metabolism</topic><topic>disease transmission</topic><topic>disease vectors</topic><topic>DNA Primers - genetics</topic><topic>Expressed Sequence Tags</topic><topic>Fatty Acids - metabolism</topic><topic>Feeding Behavior</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - physiology</topic><topic>genes</topic><topic>hematophagy</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>metabolism</topic><topic>microarray technology</topic><topic>Midgut</topic><topic>nutrient uptake</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Osmotic Pressure</topic><topic>Oxidative Stress - genetics</topic><topic>polymerase chain reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>Signal Transduction</topic><topic>stress response</topic><topic>Time Factors</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanders, Heather R.</creatorcontrib><creatorcontrib>Evans, Amy M.</creatorcontrib><creatorcontrib>Ross, Linda S.</creatorcontrib><creatorcontrib>Gill, Sarjeet S.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanders, Heather R.</au><au>Evans, Amy M.</au><au>Ross, Linda S.</au><au>Gill, Sarjeet S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2003-11</date><risdate>2003</risdate><volume>33</volume><issue>11</issue><spage>1105</spage><epage>1122</epage><pages>1105-1122</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Blood feeding is an essential developmental process for many arthropods and plays a significant role in disease transmission. Understanding physiological responses in the midgut is important because it is the primary site of blood meal digestion and pathogenic infection. Processes that occur in the midgut in response to a blood meal have been studied but are poorly understood. Here, we use cDNA microarrays to examine midgut gene expression on a global level in response to blood feeding to assist in unraveling these processes. We have developed
Aedes aegypti microarrays consisting of clones obtained from an expressed sequence tag project. Individual clones were amplified by polymerase chain reaction and printed onto glass slides. These microarrays were used to study the effects of a blood meal on midgut gene expression over a 72-h time course. As a result, a number of genes involved in processes such as nutrient uptake and metabolism, cellular stress responses, ion balance, and PM formation, as well as a number of unknown genes were induced or repressed in response to a blood meal based on this microarray data.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>14563362</pmid><doi>10.1016/S0965-1748(03)00124-3</doi><tpages>18</tpages></addata></record> |
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| subjects | Aedes - genetics Aedes - metabolism Aedes - physiology Aedes aegypti Animals arthropods Biological Transport - genetics blood Blood - metabolism Blood meal clones complementary DNA digestion Digestive System - metabolism disease transmission disease vectors DNA Primers - genetics Expressed Sequence Tags Fatty Acids - metabolism Feeding Behavior Gene expression Gene Expression Profiling Gene Expression Regulation - physiology genes hematophagy Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism metabolism microarray technology Midgut nutrient uptake Oligonucleotide Array Sequence Analysis Osmotic Pressure Oxidative Stress - genetics polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods Signal Transduction stress response Time Factors Transcription, Genetic |
| title | Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti |
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