Biochemical evidence for a P2Y-like receptor in Tetrahymena thermophila
Extracellular nucleotides are ubiquitous signaling molecules. ATP signals through two receptor types: the ionotropic P2X receptors, and the metabotropic P2Y receptors. ATP acts as a chemorepellent in Tetrahymena thermophila, where it causes a distinct avoidance response. The intracellular mechanisms...
Gespeichert in:
| Veröffentlicht in: | Journal of Comparative Physiology 2003-10, Vol.189 (10), p.781-789 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 789 |
|---|---|
| container_issue | 10 |
| container_start_page | 781 |
| container_title | Journal of Comparative Physiology |
| container_volume | 189 |
| creator | Rosner, B N Bartholomew, J N Gaines, C D Riddle, M L Everett, H A Rulapaugh, K G Nickerson, L E Marshall, M R Kuruvilla, H G |
| description | Extracellular nucleotides are ubiquitous signaling molecules. ATP signals through two receptor types: the ionotropic P2X receptors, and the metabotropic P2Y receptors. ATP acts as a chemorepellent in Tetrahymena thermophila, where it causes a distinct avoidance response. The intracellular mechanisms by which ATP causes avoidance in this organism, however, are unknown. In this study, we use in vivo pharmacological assays along with enzyme immuno-assays to obtain information about the ATP chemorepellent pathway and its associated second messenger systems. Our data show strong similarities between the presumed ATP receptor of T. thermophila and members of the P2Y family of receptors. The ATP response of T. thermophila appears to be coupled to phospholipase C, a defining characteristic of the P2Y receptor family. In addition, the ATP chemoresponse appears to be linked to a G(i/o) protein, nitric oxide synthase, and adenylyl cyclase, all of which are characteristic of some P2Y receptors. This is an important first step in describing the pathways involved in ATP chemoresponse of this organism. |
| doi_str_mv | 10.1007/s00359-003-0453-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71288793</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2084367631</sourcerecordid><originalsourceid>FETCH-LOGICAL-c324t-3398c7acfef50985006997c5dee5745915fc2bcec8e3c4a549fc1421309d34d13</originalsourceid><addsrcrecordid>eNpdkM1LAzEQxYMoWqt_gBdZPHiLTr66m6MWrUJBD_XgKaTZWXbrfpnsCv3vTWlB8PIGhvceMz9CrhjcMYD0PgAIpWlUClIJmh2RCZOCUyYUOyYTEBJoqrQ8I-chbACAM85OyRkTswyY4BOyeKw6V2JTOVsn-FPl2DpMis4nNnnnn7SuvjDx6LAf4q5qkxUO3pbbBlubDCX6puvLqrYX5KSwdcDLw5ySj-en1fyFLt8Wr_OHJXWCy4EKoTOXWldgoUBnCmCmdepUjqhSqTRTheNrhy5D4aRVUheOSc4E6FzInIkpud339r77HjEMpqmCw7q2LXZjMCnjWZZqEY03_4ybbvRtvM3M4vsaVCQ1JWxvcr4LwWNhel811m8NA7NDbPaITVSzQ2yymLk-FI_rBvO_xIGp-AWBFXU4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>613690514</pqid></control><display><type>article</type><title>Biochemical evidence for a P2Y-like receptor in Tetrahymena thermophila</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Rosner, B N ; Bartholomew, J N ; Gaines, C D ; Riddle, M L ; Everett, H A ; Rulapaugh, K G ; Nickerson, L E ; Marshall, M R ; Kuruvilla, H G</creator><creatorcontrib>Rosner, B N ; Bartholomew, J N ; Gaines, C D ; Riddle, M L ; Everett, H A ; Rulapaugh, K G ; Nickerson, L E ; Marshall, M R ; Kuruvilla, H G</creatorcontrib><description>Extracellular nucleotides are ubiquitous signaling molecules. ATP signals through two receptor types: the ionotropic P2X receptors, and the metabotropic P2Y receptors. ATP acts as a chemorepellent in Tetrahymena thermophila, where it causes a distinct avoidance response. The intracellular mechanisms by which ATP causes avoidance in this organism, however, are unknown. In this study, we use in vivo pharmacological assays along with enzyme immuno-assays to obtain information about the ATP chemorepellent pathway and its associated second messenger systems. Our data show strong similarities between the presumed ATP receptor of T. thermophila and members of the P2Y family of receptors. The ATP response of T. thermophila appears to be coupled to phospholipase C, a defining characteristic of the P2Y receptor family. In addition, the ATP chemoresponse appears to be linked to a G(i/o) protein, nitric oxide synthase, and adenylyl cyclase, all of which are characteristic of some P2Y receptors. This is an important first step in describing the pathways involved in ATP chemoresponse of this organism.</description><identifier>ISSN: 0340-7594</identifier><identifier>EISSN: 1432-1351</identifier><identifier>DOI: 10.1007/s00359-003-0453-8</identifier><identifier>PMID: 13680132</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Adenosine Triphosphate - analogs & derivatives ; Adenosine Triphosphate - metabolism ; Adenosine Triphosphate - pharmacology ; Animals ; Behavior, Animal ; Chelating Agents - pharmacology ; Dose-Response Relationship, Drug ; Egtazic Acid - analogs & derivatives ; Egtazic Acid - pharmacology ; Enzyme Inhibitors - pharmacology ; Enzyme-Linked Immunosorbent Assay ; Escape Reaction - drug effects ; Guanosine Diphosphate - analogs & derivatives ; Guanosine Diphosphate - pharmacology ; Nitric oxide ; Pertussis Toxin - pharmacology ; Proteins ; Receptors, Purinergic P2 - chemistry ; Receptors, Purinergic P2 - metabolism ; Rodents ; Second Messenger Systems - drug effects ; Tetrahymena thermophila - cytology ; Tetrahymena thermophila - metabolism ; Thionucleotides - pharmacology</subject><ispartof>Journal of Comparative Physiology, 2003-10, Vol.189 (10), p.781-789</ispartof><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-3398c7acfef50985006997c5dee5745915fc2bcec8e3c4a549fc1421309d34d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13680132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosner, B N</creatorcontrib><creatorcontrib>Bartholomew, J N</creatorcontrib><creatorcontrib>Gaines, C D</creatorcontrib><creatorcontrib>Riddle, M L</creatorcontrib><creatorcontrib>Everett, H A</creatorcontrib><creatorcontrib>Rulapaugh, K G</creatorcontrib><creatorcontrib>Nickerson, L E</creatorcontrib><creatorcontrib>Marshall, M R</creatorcontrib><creatorcontrib>Kuruvilla, H G</creatorcontrib><title>Biochemical evidence for a P2Y-like receptor in Tetrahymena thermophila</title><title>Journal of Comparative Physiology</title><addtitle>J Comp Physiol A Neuroethol Sens Neural Behav Physiol</addtitle><description>Extracellular nucleotides are ubiquitous signaling molecules. ATP signals through two receptor types: the ionotropic P2X receptors, and the metabotropic P2Y receptors. ATP acts as a chemorepellent in Tetrahymena thermophila, where it causes a distinct avoidance response. The intracellular mechanisms by which ATP causes avoidance in this organism, however, are unknown. In this study, we use in vivo pharmacological assays along with enzyme immuno-assays to obtain information about the ATP chemorepellent pathway and its associated second messenger systems. Our data show strong similarities between the presumed ATP receptor of T. thermophila and members of the P2Y family of receptors. The ATP response of T. thermophila appears to be coupled to phospholipase C, a defining characteristic of the P2Y receptor family. In addition, the ATP chemoresponse appears to be linked to a G(i/o) protein, nitric oxide synthase, and adenylyl cyclase, all of which are characteristic of some P2Y receptors. This is an important first step in describing the pathways involved in ATP chemoresponse of this organism.</description><subject>Adenosine Triphosphate - analogs & derivatives</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Behavior, Animal</subject><subject>Chelating Agents - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Egtazic Acid - analogs & derivatives</subject><subject>Egtazic Acid - pharmacology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escape Reaction - drug effects</subject><subject>Guanosine Diphosphate - analogs & derivatives</subject><subject>Guanosine Diphosphate - pharmacology</subject><subject>Nitric oxide</subject><subject>Pertussis Toxin - pharmacology</subject><subject>Proteins</subject><subject>Receptors, Purinergic P2 - chemistry</subject><subject>Receptors, Purinergic P2 - metabolism</subject><subject>Rodents</subject><subject>Second Messenger Systems - drug effects</subject><subject>Tetrahymena thermophila - cytology</subject><subject>Tetrahymena thermophila - metabolism</subject><subject>Thionucleotides - pharmacology</subject><issn>0340-7594</issn><issn>1432-1351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkM1LAzEQxYMoWqt_gBdZPHiLTr66m6MWrUJBD_XgKaTZWXbrfpnsCv3vTWlB8PIGhvceMz9CrhjcMYD0PgAIpWlUClIJmh2RCZOCUyYUOyYTEBJoqrQ8I-chbACAM85OyRkTswyY4BOyeKw6V2JTOVsn-FPl2DpMis4nNnnnn7SuvjDx6LAf4q5qkxUO3pbbBlubDCX6puvLqrYX5KSwdcDLw5ySj-en1fyFLt8Wr_OHJXWCy4EKoTOXWldgoUBnCmCmdepUjqhSqTRTheNrhy5D4aRVUheOSc4E6FzInIkpud339r77HjEMpqmCw7q2LXZjMCnjWZZqEY03_4ybbvRtvM3M4vsaVCQ1JWxvcr4LwWNhel811m8NA7NDbPaITVSzQ2yymLk-FI_rBvO_xIGp-AWBFXU4</recordid><startdate>200310</startdate><enddate>200310</enddate><creator>Rosner, B N</creator><creator>Bartholomew, J N</creator><creator>Gaines, C D</creator><creator>Riddle, M L</creator><creator>Everett, H A</creator><creator>Rulapaugh, K G</creator><creator>Nickerson, L E</creator><creator>Marshall, M R</creator><creator>Kuruvilla, H G</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200310</creationdate><title>Biochemical evidence for a P2Y-like receptor in Tetrahymena thermophila</title><author>Rosner, B N ; Bartholomew, J N ; Gaines, C D ; Riddle, M L ; Everett, H A ; Rulapaugh, K G ; Nickerson, L E ; Marshall, M R ; Kuruvilla, H G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-3398c7acfef50985006997c5dee5745915fc2bcec8e3c4a549fc1421309d34d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adenosine Triphosphate - analogs & derivatives</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Behavior, Animal</topic><topic>Chelating Agents - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Egtazic Acid - analogs & derivatives</topic><topic>Egtazic Acid - pharmacology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Escape Reaction - drug effects</topic><topic>Guanosine Diphosphate - analogs & derivatives</topic><topic>Guanosine Diphosphate - pharmacology</topic><topic>Nitric oxide</topic><topic>Pertussis Toxin - pharmacology</topic><topic>Proteins</topic><topic>Receptors, Purinergic P2 - chemistry</topic><topic>Receptors, Purinergic P2 - metabolism</topic><topic>Rodents</topic><topic>Second Messenger Systems - drug effects</topic><topic>Tetrahymena thermophila - cytology</topic><topic>Tetrahymena thermophila - metabolism</topic><topic>Thionucleotides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosner, B N</creatorcontrib><creatorcontrib>Bartholomew, J N</creatorcontrib><creatorcontrib>Gaines, C D</creatorcontrib><creatorcontrib>Riddle, M L</creatorcontrib><creatorcontrib>Everett, H A</creatorcontrib><creatorcontrib>Rulapaugh, K G</creatorcontrib><creatorcontrib>Nickerson, L E</creatorcontrib><creatorcontrib>Marshall, M R</creatorcontrib><creatorcontrib>Kuruvilla, H G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Comparative Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosner, B N</au><au>Bartholomew, J N</au><au>Gaines, C D</au><au>Riddle, M L</au><au>Everett, H A</au><au>Rulapaugh, K G</au><au>Nickerson, L E</au><au>Marshall, M R</au><au>Kuruvilla, H G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biochemical evidence for a P2Y-like receptor in Tetrahymena thermophila</atitle><jtitle>Journal of Comparative Physiology</jtitle><addtitle>J Comp Physiol A Neuroethol Sens Neural Behav Physiol</addtitle><date>2003-10</date><risdate>2003</risdate><volume>189</volume><issue>10</issue><spage>781</spage><epage>789</epage><pages>781-789</pages><issn>0340-7594</issn><eissn>1432-1351</eissn><abstract>Extracellular nucleotides are ubiquitous signaling molecules. ATP signals through two receptor types: the ionotropic P2X receptors, and the metabotropic P2Y receptors. ATP acts as a chemorepellent in Tetrahymena thermophila, where it causes a distinct avoidance response. The intracellular mechanisms by which ATP causes avoidance in this organism, however, are unknown. In this study, we use in vivo pharmacological assays along with enzyme immuno-assays to obtain information about the ATP chemorepellent pathway and its associated second messenger systems. Our data show strong similarities between the presumed ATP receptor of T. thermophila and members of the P2Y family of receptors. The ATP response of T. thermophila appears to be coupled to phospholipase C, a defining characteristic of the P2Y receptor family. In addition, the ATP chemoresponse appears to be linked to a G(i/o) protein, nitric oxide synthase, and adenylyl cyclase, all of which are characteristic of some P2Y receptors. This is an important first step in describing the pathways involved in ATP chemoresponse of this organism.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>13680132</pmid><doi>10.1007/s00359-003-0453-8</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0340-7594 |
| ispartof | Journal of Comparative Physiology, 2003-10, Vol.189 (10), p.781-789 |
| issn | 0340-7594 1432-1351 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71288793 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - metabolism Adenosine Triphosphate - pharmacology Animals Behavior, Animal Chelating Agents - pharmacology Dose-Response Relationship, Drug Egtazic Acid - analogs & derivatives Egtazic Acid - pharmacology Enzyme Inhibitors - pharmacology Enzyme-Linked Immunosorbent Assay Escape Reaction - drug effects Guanosine Diphosphate - analogs & derivatives Guanosine Diphosphate - pharmacology Nitric oxide Pertussis Toxin - pharmacology Proteins Receptors, Purinergic P2 - chemistry Receptors, Purinergic P2 - metabolism Rodents Second Messenger Systems - drug effects Tetrahymena thermophila - cytology Tetrahymena thermophila - metabolism Thionucleotides - pharmacology |
| title | Biochemical evidence for a P2Y-like receptor in Tetrahymena thermophila |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A42%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biochemical%20evidence%20for%20a%20P2Y-like%20receptor%20in%20Tetrahymena%20thermophila&rft.jtitle=Journal%20of%20Comparative%20Physiology&rft.au=Rosner,%20B%20N&rft.date=2003-10&rft.volume=189&rft.issue=10&rft.spage=781&rft.epage=789&rft.pages=781-789&rft.issn=0340-7594&rft.eissn=1432-1351&rft_id=info:doi/10.1007/s00359-003-0453-8&rft_dat=%3Cproquest_cross%3E2084367631%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=613690514&rft_id=info:pmid/13680132&rfr_iscdi=true |