Role of Gregarine Parasite Ascogregarina culicis (Apicomplexa: Lecudinidae) in the Maintenance of Chikungunya Virus in Vector Mosquito
Ascogregarina culicis and Ascogregarina taiwanensis are common gregarine parasites of Aedes aegypti and Aedes albopictus mosquitoes, respectively. These mosquito species are also known to transmit dengue and Chikungunya viruses. The sporozoites of these parasites invade the midgut epithelial cells a...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 2003-09, Vol.50 (5), p.379-382 |
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| creator | MOURYA, D. T SINGH, D. K YADAV, P GOKHALE, M. D BARDE, P. V NARAYAN, N. B THAKARE, J. P MISHRA, A. C SHOUCHE, Y. S |
| description | Ascogregarina culicis and Ascogregarina taiwanensis are common gregarine parasites of Aedes aegypti and Aedes albopictus mosquitoes, respectively. These mosquito species are also known to transmit dengue and Chikungunya viruses. The sporozoites of these parasites invade the midgut epithelial cells and develop intracellularly and extracellularly in the gut to complete their life cycles. The midgut is also the primary site for virus replication in the vector mosquitoes. Therefore, studies were carried out with a view to determine the possible role of these gregarines in the vertical transmission of dengue and Chikungunya viruses from larval to adult stage. Experiments were performed by exposing first instar mosquito larvae to suspensions containing parasite oocysts and viruses. Since Ascogregarina sporozoites invade the midgut of first instar larvae, the vertical transmission was determined by feeding the uninfected first instar larvae on the freshly prepared homogenates from mosquitoes, which were dually infected with viruses and the parasite oocysts. Similarly, the role of protozoan parasites in the vertical transmission of viruses was determined by exposing fresh first instar larvae to the dried pellets of homogenates prepared from the mosquitoes dually infected with viruses and the parasite oocysts. Direct vertical transmission and the vertical transmission of CHIK virus through the oocyst of the parasites were observed in the case of Ae. aegypti mosquitoes. It is suggested that As. culicis may have an important role in the maintenance of CHIK virus during the inter-epidemic period. |
| doi_str_mv | 10.1111/j.1550-7408.2003.tb00153.x |
| format | Article |
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T ; SINGH, D. K ; YADAV, P ; GOKHALE, M. D ; BARDE, P. V ; NARAYAN, N. B ; THAKARE, J. P ; MISHRA, A. C ; SHOUCHE, Y. S</creator><creatorcontrib>MOURYA, D. T ; SINGH, D. K ; YADAV, P ; GOKHALE, M. D ; BARDE, P. V ; NARAYAN, N. B ; THAKARE, J. P ; MISHRA, A. C ; SHOUCHE, Y. S</creatorcontrib><description>Ascogregarina culicis and Ascogregarina taiwanensis are common gregarine parasites of Aedes aegypti and Aedes albopictus mosquitoes, respectively. These mosquito species are also known to transmit dengue and Chikungunya viruses. The sporozoites of these parasites invade the midgut epithelial cells and develop intracellularly and extracellularly in the gut to complete their life cycles. The midgut is also the primary site for virus replication in the vector mosquitoes. Therefore, studies were carried out with a view to determine the possible role of these gregarines in the vertical transmission of dengue and Chikungunya viruses from larval to adult stage. Experiments were performed by exposing first instar mosquito larvae to suspensions containing parasite oocysts and viruses. Since Ascogregarina sporozoites invade the midgut of first instar larvae, the vertical transmission was determined by feeding the uninfected first instar larvae on the freshly prepared homogenates from mosquitoes, which were dually infected with viruses and the parasite oocysts. Similarly, the role of protozoan parasites in the vertical transmission of viruses was determined by exposing fresh first instar larvae to the dried pellets of homogenates prepared from the mosquitoes dually infected with viruses and the parasite oocysts. Direct vertical transmission and the vertical transmission of CHIK virus through the oocyst of the parasites were observed in the case of Ae. aegypti mosquitoes. It is suggested that As. culicis may have an important role in the maintenance of CHIK virus during the inter-epidemic period.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.2003.tb00153.x</identifier><identifier>PMID: 14563178</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>A. albopictus ; Aedes - parasitology ; Aedes - virology ; Aedes aegypti ; Alphavirus Infections - transmission ; Animals ; Antigens, Viral - analysis ; Apicomplexa - virology ; Biological and medical sciences ; CHIK virus ; Chikungunya virus - genetics ; Chikungunya virus - growth & development ; dengue ; DNA, Viral - chemistry ; DNA, Viral - genetics ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; gregarine oocyst ; Infectious Disease Transmission, Vertical ; Insect Vectors - virology ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; Mice ; mosquito-man-mosquito virus cycle ; Polymerase Chain Reaction ; protozoan parasite ; Vectors. Intermediate hosts ; vertical transmission ; virus maintenance ; Virus Replication - physiology</subject><ispartof>The Journal of eukaryotic microbiology, 2003-09, Vol.50 (5), p.379-382</ispartof><rights>Society of Protozoologists</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b4879-febeb68beb135418f7306cd9c34af166ccf45563803c6c514a4f5dcd6335a67e3</citedby><cites>FETCH-LOGICAL-b4879-febeb68beb135418f7306cd9c34af166ccf45563803c6c514a4f5dcd6335a67e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1111/j.1550-7408.2003.tb00153.x$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1550-7408.2003.tb00153.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,26978,27924,27925,45574,45575,52363</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15160458$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14563178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOURYA, D. T</creatorcontrib><creatorcontrib>SINGH, D. K</creatorcontrib><creatorcontrib>YADAV, P</creatorcontrib><creatorcontrib>GOKHALE, M. D</creatorcontrib><creatorcontrib>BARDE, P. V</creatorcontrib><creatorcontrib>NARAYAN, N. B</creatorcontrib><creatorcontrib>THAKARE, J. P</creatorcontrib><creatorcontrib>MISHRA, A. C</creatorcontrib><creatorcontrib>SHOUCHE, Y. S</creatorcontrib><title>Role of Gregarine Parasite Ascogregarina culicis (Apicomplexa: Lecudinidae) in the Maintenance of Chikungunya Virus in Vector Mosquito</title><title>The Journal of eukaryotic microbiology</title><addtitle>J Eukaryot Microbiol</addtitle><description>Ascogregarina culicis and Ascogregarina taiwanensis are common gregarine parasites of Aedes aegypti and Aedes albopictus mosquitoes, respectively. These mosquito species are also known to transmit dengue and Chikungunya viruses. The sporozoites of these parasites invade the midgut epithelial cells and develop intracellularly and extracellularly in the gut to complete their life cycles. The midgut is also the primary site for virus replication in the vector mosquitoes. Therefore, studies were carried out with a view to determine the possible role of these gregarines in the vertical transmission of dengue and Chikungunya viruses from larval to adult stage. Experiments were performed by exposing first instar mosquito larvae to suspensions containing parasite oocysts and viruses. Since Ascogregarina sporozoites invade the midgut of first instar larvae, the vertical transmission was determined by feeding the uninfected first instar larvae on the freshly prepared homogenates from mosquitoes, which were dually infected with viruses and the parasite oocysts. Similarly, the role of protozoan parasites in the vertical transmission of viruses was determined by exposing fresh first instar larvae to the dried pellets of homogenates prepared from the mosquitoes dually infected with viruses and the parasite oocysts. Direct vertical transmission and the vertical transmission of CHIK virus through the oocyst of the parasites were observed in the case of Ae. aegypti mosquitoes. It is suggested that As. culicis may have an important role in the maintenance of CHIK virus during the inter-epidemic period.</description><subject>A. albopictus</subject><subject>Aedes - parasitology</subject><subject>Aedes - virology</subject><subject>Aedes aegypti</subject><subject>Alphavirus Infections - transmission</subject><subject>Animals</subject><subject>Antigens, Viral - analysis</subject><subject>Apicomplexa - virology</subject><subject>Biological and medical sciences</subject><subject>CHIK virus</subject><subject>Chikungunya virus - genetics</subject><subject>Chikungunya virus - growth & development</subject><subject>dengue</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - genetics</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gregarine oocyst</subject><subject>Infectious Disease Transmission, Vertical</subject><subject>Insect Vectors - virology</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>Mice</subject><subject>mosquito-man-mosquito virus cycle</subject><subject>Polymerase Chain Reaction</subject><subject>protozoan parasite</subject><subject>Vectors. Intermediate hosts</subject><subject>vertical transmission</subject><subject>virus maintenance</subject><subject>Virus Replication - physiology</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd9u0zAUxi0EYqPwCshCArGLBLv-k3QXSFU3OqYO0MQKd5bjnHTu0rizE9G-AM-NQ6Pter6wj-zf-Y7PdxB6R0lK4_q0TqkQJMk4ydMxISxtC0KoYOnuGTp-eHoeYyJlIsaMH6FXIawjJMeUvkRHlAvJaJYfo7_XrgbsKjz3sNLeNoB_aK-DbQFPg3Gr4Vpj09XW2IA_TrfWuM22hp0-xQswXWkbW2o4wbbB7S3gK22bFhrdmP_Ks1t71zWrrtlrvLS-Cz23BNM6j69cuO9s616jF5WuA7wZzhG6-XL-c3aRLL7Pv86mi6TgeTZJKiigkHncKBOc5lXGiDTlxDCuKyqlMRUXsbWcMCONoFzzSpSmlIwJLTNgI_ThoLv17r6D0KqNDQbqWjfguqAyOo51Ij5CpwfQeBeCh0ptvd1ov1eUqH4Kaq16q1VvteqnoIYpqF1MfjtU6YoNlI-pg-0ReD8AOhhdVz56ZcMjJ6gkXPTc5wP3x9awf8IX1OX5DcsmUSA5CNjQwu5BQPs7JTOWCfXr21xd8mu-XP4-UxeRzw98YZ1r4ClN_wNp18Zj</recordid><startdate>200309</startdate><enddate>200309</enddate><creator>MOURYA, D. T</creator><creator>SINGH, D. K</creator><creator>YADAV, P</creator><creator>GOKHALE, M. D</creator><creator>BARDE, P. V</creator><creator>NARAYAN, N. B</creator><creator>THAKARE, J. P</creator><creator>MISHRA, A. C</creator><creator>SHOUCHE, Y. S</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200309</creationdate><title>Role of Gregarine Parasite Ascogregarina culicis (Apicomplexa: Lecudinidae) in the Maintenance of Chikungunya Virus in Vector Mosquito</title><author>MOURYA, D. T ; SINGH, D. K ; YADAV, P ; GOKHALE, M. D ; BARDE, P. V ; NARAYAN, N. B ; THAKARE, J. P ; MISHRA, A. C ; SHOUCHE, Y. 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Psychology</topic><topic>gregarine oocyst</topic><topic>Infectious Disease Transmission, Vertical</topic><topic>Insect Vectors - virology</topic><topic>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</topic><topic>Mice</topic><topic>mosquito-man-mosquito virus cycle</topic><topic>Polymerase Chain Reaction</topic><topic>protozoan parasite</topic><topic>Vectors. Intermediate hosts</topic><topic>vertical transmission</topic><topic>virus maintenance</topic><topic>Virus Replication - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOURYA, D. T</creatorcontrib><creatorcontrib>SINGH, D. K</creatorcontrib><creatorcontrib>YADAV, P</creatorcontrib><creatorcontrib>GOKHALE, M. D</creatorcontrib><creatorcontrib>BARDE, P. V</creatorcontrib><creatorcontrib>NARAYAN, N. B</creatorcontrib><creatorcontrib>THAKARE, J. P</creatorcontrib><creatorcontrib>MISHRA, A. 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S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Gregarine Parasite Ascogregarina culicis (Apicomplexa: Lecudinidae) in the Maintenance of Chikungunya Virus in Vector Mosquito</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>2003-09</date><risdate>2003</risdate><volume>50</volume><issue>5</issue><spage>379</spage><epage>382</epage><pages>379-382</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>Ascogregarina culicis and Ascogregarina taiwanensis are common gregarine parasites of Aedes aegypti and Aedes albopictus mosquitoes, respectively. These mosquito species are also known to transmit dengue and Chikungunya viruses. The sporozoites of these parasites invade the midgut epithelial cells and develop intracellularly and extracellularly in the gut to complete their life cycles. The midgut is also the primary site for virus replication in the vector mosquitoes. Therefore, studies were carried out with a view to determine the possible role of these gregarines in the vertical transmission of dengue and Chikungunya viruses from larval to adult stage. Experiments were performed by exposing first instar mosquito larvae to suspensions containing parasite oocysts and viruses. Since Ascogregarina sporozoites invade the midgut of first instar larvae, the vertical transmission was determined by feeding the uninfected first instar larvae on the freshly prepared homogenates from mosquitoes, which were dually infected with viruses and the parasite oocysts. Similarly, the role of protozoan parasites in the vertical transmission of viruses was determined by exposing fresh first instar larvae to the dried pellets of homogenates prepared from the mosquitoes dually infected with viruses and the parasite oocysts. Direct vertical transmission and the vertical transmission of CHIK virus through the oocyst of the parasites were observed in the case of Ae. aegypti mosquitoes. It is suggested that As. culicis may have an important role in the maintenance of CHIK virus during the inter-epidemic period.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>14563178</pmid><doi>10.1111/j.1550-7408.2003.tb00153.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | The Journal of eukaryotic microbiology, 2003-09, Vol.50 (5), p.379-382 |
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| subjects | A. albopictus Aedes - parasitology Aedes - virology Aedes aegypti Alphavirus Infections - transmission Animals Antigens, Viral - analysis Apicomplexa - virology Biological and medical sciences CHIK virus Chikungunya virus - genetics Chikungunya virus - growth & development dengue DNA, Viral - chemistry DNA, Viral - genetics Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology gregarine oocyst Infectious Disease Transmission, Vertical Insect Vectors - virology Medically important nuisances and vectors, pests of stored products and materials: population survey and control Mice mosquito-man-mosquito virus cycle Polymerase Chain Reaction protozoan parasite Vectors. Intermediate hosts vertical transmission virus maintenance Virus Replication - physiology |
| title | Role of Gregarine Parasite Ascogregarina culicis (Apicomplexa: Lecudinidae) in the Maintenance of Chikungunya Virus in Vector Mosquito |
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