Identification of Proteins in Single-Cell Capillary Electrophoresis Fingerprints Based on Comigration with Standard Proteins

In the previous paper in this Journal, we reported the use of capillary sieving electrophoresis to characterize proteins expressed by single cancer cells at specific phases in the cell cycle. Analysis of the data revealed one component with cell cycle-dependent changes in expression at the 99% confi...

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Veröffentlicht in:	Analytical chemistry (Washington) 2003-07, Vol.75 (14), p.3502-3505
Hauptverfasser:	Hu, Shen, Zhang, Le, Newitt, Richard, Aebersold, Ruedi, Kraly, James R, Jones, Megan, Dovichi, Norman J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Electrophoresis, Capillary Exact sciences and technology Fundamental and applied biological sciences. Psychology HT29 Cells Humans Indicators and Reagents Mass Spectrometry Molecular Sequence Data Other chromatographic methods Peptide Mapping Protein Hydrolysates - chemistry Proteins - chemistry Reference Standards Spectrometric and optical methods Trypsin - chemistry
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