A 15-s Protein Separation Employing Hydrodynamic Force on a Microchip

We report here a novel pressurization technique for microchip electrophoresis that enables 15-s separation of protein mixtures extracted from biological samples. Although pressure-driven flow is usually parabolic flow, pressurization prior to electrophoresis separation produced a plug flow and achie...

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Veröffentlicht in:	Analytical chemistry (Washington) 2003-08, Vol.75 (15), p.3799-3805
Hauptverfasser:	Tabuchi, Mari, Kuramitsu, Yasuhiro, Nakamura, Kazuyuki, Baba, Yoshinobu
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Computer Simulation Electrophoresis, Gel, Two-Dimensional - methods Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation methods Humans Isoelectric Focusing - methods Jurkat Cells microchips Other chromatographic methods Pressure Protein Array Analysis - methods Proteins Proteins - chemistry Semiconductors Temperature Time Factors
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