Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers
Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD‐modified silk matrix was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrate...
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| Veröffentlicht in: | Journal of biomedical materials research 2003-11, Vol.67A (2), p.559-570 |
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| container_title | Journal of biomedical materials research |
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| creator | Chen, Jingsong Altman, Gregory H. Karageorgiou, Vassilis Horan, Rebecca Collette, Adam Volloch, Vladimir Colabro, Tara Kaplan, David L. |
| description | Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD‐modified silk matrix was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non‐RGD‐modified matrices. Collagen type I transcript levels (at day 7) and content (at day 14) was significantly higher on the RGD‐modified substrate than on the nonmodified group. The ability of RGD‐coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 559–570, 2003 |
| doi_str_mv | 10.1002/jbm.a.10120 |
| format | Article |
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Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non‐RGD‐modified matrices. Collagen type I transcript levels (at day 7) and content (at day 14) was significantly higher on the RGD‐modified substrate than on the nonmodified group. The ability of RGD‐coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 559–570, 2003</description><identifier>ISSN: 1549-3296</identifier><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1552-4965</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/jbm.a.10120</identifier><identifier>PMID: 14566798</identifier><identifier>CODEN: JBMRBG</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biocompatible Materials ; Biological and medical sciences ; biomaterial ; Bombyx ; Bone Marrow Cells - metabolism ; bone marrow stromal cells ; Cell Adhesion ; Fibroblasts - metabolism ; Humans ; Insect Proteins ; ligament tissue engineering ; Ligaments - metabolism ; Medical sciences ; Microscopy, Electron, Scanning ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; RGD ; Silk ; Stromal Cells - metabolism ; Technology. Biomaterials. Equipments. Material. Instrumentation ; Tissue Engineering</subject><ispartof>Journal of biomedical materials research, 2003-11, Vol.67A (2), p.559-570</ispartof><rights>Copyright © 2003 Wiley Periodicals, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2003 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4920-6cdca5f20e0dd992a18c90be2555a016575363e21783ecc7c000213db00a857d3</citedby><cites>FETCH-LOGICAL-c4920-6cdca5f20e0dd992a18c90be2555a016575363e21783ecc7c000213db00a857d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.10120$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.10120$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15246955$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14566798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Jingsong</creatorcontrib><creatorcontrib>Altman, Gregory H.</creatorcontrib><creatorcontrib>Karageorgiou, Vassilis</creatorcontrib><creatorcontrib>Horan, Rebecca</creatorcontrib><creatorcontrib>Collette, Adam</creatorcontrib><creatorcontrib>Volloch, Vladimir</creatorcontrib><creatorcontrib>Colabro, Tara</creatorcontrib><creatorcontrib>Kaplan, David L.</creatorcontrib><title>Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers</title><title>Journal of biomedical materials research</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD‐modified silk matrix was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non‐RGD‐modified matrices. Collagen type I transcript levels (at day 7) and content (at day 14) was significantly higher on the RGD‐modified substrate than on the nonmodified group. The ability of RGD‐coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 559–570, 2003</description><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Biological and medical sciences</subject><subject>biomaterial</subject><subject>Bombyx</subject><subject>Bone Marrow Cells - metabolism</subject><subject>bone marrow stromal cells</subject><subject>Cell Adhesion</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Insect Proteins</subject><subject>ligament tissue engineering</subject><subject>Ligaments - metabolism</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>RGD</subject><subject>Silk</subject><subject>Stromal Cells - metabolism</subject><subject>Technology. Biomaterials. Equipments. Material. 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Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>RGD</topic><topic>Silk</topic><topic>Stromal Cells - metabolism</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Jingsong</creatorcontrib><creatorcontrib>Altman, Gregory H.</creatorcontrib><creatorcontrib>Karageorgiou, Vassilis</creatorcontrib><creatorcontrib>Horan, Rebecca</creatorcontrib><creatorcontrib>Collette, Adam</creatorcontrib><creatorcontrib>Volloch, Vladimir</creatorcontrib><creatorcontrib>Colabro, Tara</creatorcontrib><creatorcontrib>Kaplan, David L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Jingsong</au><au>Altman, Gregory H.</au><au>Karageorgiou, Vassilis</au><au>Horan, Rebecca</au><au>Collette, Adam</au><au>Volloch, Vladimir</au><au>Colabro, Tara</au><au>Kaplan, David L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers</atitle><jtitle>Journal of biomedical materials research</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>67A</volume><issue>2</issue><spage>559</spage><epage>570</epage><pages>559-570</pages><issn>1549-3296</issn><issn>0021-9304</issn><eissn>1552-4965</eissn><eissn>1097-4636</eissn><coden>JBMRBG</coden><abstract>Adhesion, spreading, proliferation, and collagen matrix production of human bone marrow stromal cells (BMSCs) on an RGD‐modified silk matrix was studied. Anterior cruciate ligament fibroblasts (ACLFs) were used as a control cell source. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the modified silk matrices support improved BMSC and ACLF attachment and show higher cell density over 14 days in culture when compared with the non‐RGD‐modified matrices. Collagen type I transcript levels (at day 7) and content (at day 14) was significantly higher on the RGD‐modified substrate than on the nonmodified group. The ability of RGD‐coupled silk matrices to support BMSC attachment, which leads to higher cell density and collagen matrix production in vitro, combined with mechanical, fatigue, and biocompatibility properties of the silk protein matrix, suggest potential for use of this biomaterial for tissue engineering. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 559–570, 2003</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>14566798</pmid><doi>10.1002/jbm.a.10120</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Biocompatible Materials Biological and medical sciences biomaterial Bombyx Bone Marrow Cells - metabolism bone marrow stromal cells Cell Adhesion Fibroblasts - metabolism Humans Insect Proteins ligament tissue engineering Ligaments - metabolism Medical sciences Microscopy, Electron, Scanning Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) RGD Silk Stromal Cells - metabolism Technology. Biomaterials. Equipments. Material. Instrumentation Tissue Engineering |
| title | Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers |
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