cDNA Cloning and mRNA Analysis of PGC-1 in Epitrochlearis Muscle in Swimming-Exercised Rats
Peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1), a cold-inducible coactivator of nuclear receptors, stimulates mitochondrial biogenesis and respiration in muscle cells. In the present study, we first cloned a rat PGC-1 gene from a brown adipose tissue cDNA library which encodes a...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-08, Vol.274 (2), p.350-354 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Goto, Masahide Terada, Shin Kato, Miyuki Katoh, Masao Yokozeki, Toshiko Tabata, Izumi Shimokawa, Teruhiko |
| description | Peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1), a cold-inducible coactivator of nuclear receptors, stimulates mitochondrial biogenesis and respiration in muscle cells. In the present study, we first cloned a rat PGC-1 gene from a brown adipose tissue cDNA library which encodes a predicted 796-amino-acid protein and exhibits respectively 98% and 95% identity with the mouse and human homologues. Next, we examined the effect of swimming exercise training on the level of expression of the PGC-1 gene in rat epitrochlearis (Epi) muscle. PGC-1 mRNA level in Epi muscle in rats that swam 2 h a day for 3 and 7 days increased dramatically by 154% and 163%, respectively, compared to the non-exercised control group. PGC-1 mRNA up-regulation was not observed in an immersion group treated at 35°C during the training program but without swimming exercise. These results demonstrate that expression of the PGC-1 gene in Epi muscle is induced not only by cold exposure but also by prolonged low-intensity physical exercise. |
| doi_str_mv | 10.1006/bbrc.2000.3134 |
| format | Article |
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In the present study, we first cloned a rat PGC-1 gene from a brown adipose tissue cDNA library which encodes a predicted 796-amino-acid protein and exhibits respectively 98% and 95% identity with the mouse and human homologues. Next, we examined the effect of swimming exercise training on the level of expression of the PGC-1 gene in rat epitrochlearis (Epi) muscle. PGC-1 mRNA level in Epi muscle in rats that swam 2 h a day for 3 and 7 days increased dramatically by 154% and 163%, respectively, compared to the non-exercised control group. PGC-1 mRNA up-regulation was not observed in an immersion group treated at 35°C during the training program but without swimming exercise. 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In the present study, we first cloned a rat PGC-1 gene from a brown adipose tissue cDNA library which encodes a predicted 796-amino-acid protein and exhibits respectively 98% and 95% identity with the mouse and human homologues. Next, we examined the effect of swimming exercise training on the level of expression of the PGC-1 gene in rat epitrochlearis (Epi) muscle. PGC-1 mRNA level in Epi muscle in rats that swam 2 h a day for 3 and 7 days increased dramatically by 154% and 163%, respectively, compared to the non-exercised control group. PGC-1 mRNA up-regulation was not observed in an immersion group treated at 35°C during the training program but without swimming exercise. These results demonstrate that expression of the PGC-1 gene in Epi muscle is induced not only by cold exposure but also by prolonged low-intensity physical exercise.</description><subject>Animals</subject><subject>Cloning, Molecular</subject><subject>cold-inducible coactivator</subject><subject>Deoxyglucose - metabolism</subject><subject>DNA, Complementary - genetics</subject><subject>epitrochlearis muscle</subject><subject>Male</subject><subject>mitochondriogenesis</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Peroxisome proliferator-activated receptor ^g coactivator 1</subject><subject>PGC-1</subject><subject>PGC-1 gene</subject><subject>Physical Conditioning, Animal - physiology</subject><subject>Physical Exertion - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sequence Homology, Amino Acid</subject><subject>Space life sciences</subject><subject>Swimming - physiology</subject><subject>swimming training</subject><subject>Temperature</subject><subject>thermogenesis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Up-Regulation - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPGzEUha0K1IS02y6rWbGb4Dv22PEyCmlACg9BkZBYWB77pnU1j2BPCvx7PAoLNojVle75zll8hPwAOgVKxUlVBTstKKVTBox_IWOgiuYFUH5Axukt8kLB_YgcxfiPUgAu1FcyShAwxosxebCnl_NsUXetb_9kpnVZc5Me89bUL9HHrNtk16tFDplvs-XW96Gzf2s0IUUXu2hrHILbJ980qZ8vnzFYH9FlN6aP38jhxtQRv7_dCbn7tfy9OMvXV6vzxXydW85knyN1QhWVEk7OKBMUGRhqWMmNdDATXDhRpJArdCVTWFonsarsRhVSitIpNiHH-91t6B53GHvd-Gixrk2L3S5qCYVkAOWnIMiyVKB4Aqd70IYuxoAbvQ2-MeFFA9WDdz1414N3PXhPhZ9vy7uqQfcO34tOwGwPYBLx32PQ0XpsLTof0Pbadf6j7VcM2485</recordid><startdate>20000802</startdate><enddate>20000802</enddate><creator>Goto, Masahide</creator><creator>Terada, Shin</creator><creator>Kato, Miyuki</creator><creator>Katoh, Masao</creator><creator>Yokozeki, Toshiko</creator><creator>Tabata, Izumi</creator><creator>Shimokawa, Teruhiko</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20000802</creationdate><title>cDNA Cloning and mRNA Analysis of PGC-1 in Epitrochlearis Muscle in Swimming-Exercised Rats</title><author>Goto, Masahide ; Terada, Shin ; Kato, Miyuki ; Katoh, Masao ; Yokozeki, Toshiko ; Tabata, Izumi ; Shimokawa, Teruhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-e0d692b96d780360e31a0a354a7d18646d6296d49ed539e5cd7ebbcf927765d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Cloning, Molecular</topic><topic>cold-inducible coactivator</topic><topic>Deoxyglucose - metabolism</topic><topic>DNA, Complementary - genetics</topic><topic>epitrochlearis muscle</topic><topic>Male</topic><topic>mitochondriogenesis</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Peroxisome proliferator-activated receptor ^g coactivator 1</topic><topic>PGC-1</topic><topic>PGC-1 gene</topic><topic>Physical Conditioning, Animal - physiology</topic><topic>Physical Exertion - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Sequence Homology, Amino Acid</topic><topic>Space life sciences</topic><topic>Swimming - physiology</topic><topic>swimming training</topic><topic>Temperature</topic><topic>thermogenesis</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Up-Regulation - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goto, Masahide</creatorcontrib><creatorcontrib>Terada, Shin</creatorcontrib><creatorcontrib>Kato, Miyuki</creatorcontrib><creatorcontrib>Katoh, Masao</creatorcontrib><creatorcontrib>Yokozeki, Toshiko</creatorcontrib><creatorcontrib>Tabata, Izumi</creatorcontrib><creatorcontrib>Shimokawa, Teruhiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goto, Masahide</au><au>Terada, Shin</au><au>Kato, Miyuki</au><au>Katoh, Masao</au><au>Yokozeki, Toshiko</au><au>Tabata, Izumi</au><au>Shimokawa, Teruhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA Cloning and mRNA Analysis of PGC-1 in Epitrochlearis Muscle in Swimming-Exercised Rats</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2000-08-02</date><risdate>2000</risdate><volume>274</volume><issue>2</issue><spage>350</spage><epage>354</epage><pages>350-354</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1), a cold-inducible coactivator of nuclear receptors, stimulates mitochondrial biogenesis and respiration in muscle cells. In the present study, we first cloned a rat PGC-1 gene from a brown adipose tissue cDNA library which encodes a predicted 796-amino-acid protein and exhibits respectively 98% and 95% identity with the mouse and human homologues. Next, we examined the effect of swimming exercise training on the level of expression of the PGC-1 gene in rat epitrochlearis (Epi) muscle. PGC-1 mRNA level in Epi muscle in rats that swam 2 h a day for 3 and 7 days increased dramatically by 154% and 163%, respectively, compared to the non-exercised control group. PGC-1 mRNA up-regulation was not observed in an immersion group treated at 35°C during the training program but without swimming exercise. These results demonstrate that expression of the PGC-1 gene in Epi muscle is induced not only by cold exposure but also by prolonged low-intensity physical exercise.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10913342</pmid><doi>10.1006/bbrc.2000.3134</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Cloning, Molecular cold-inducible coactivator Deoxyglucose - metabolism DNA, Complementary - genetics epitrochlearis muscle Male mitochondriogenesis Molecular Sequence Data Muscle, Skeletal - metabolism Peroxisome proliferator-activated receptor ^g coactivator 1 PGC-1 PGC-1 gene Physical Conditioning, Animal - physiology Physical Exertion - physiology Rats Rats, Sprague-Dawley RNA, Messenger - analysis RNA, Messenger - biosynthesis Sequence Homology, Amino Acid Space life sciences Swimming - physiology swimming training Temperature thermogenesis Transcription Factors - genetics Transcription Factors - metabolism Up-Regulation - genetics |
| title | cDNA Cloning and mRNA Analysis of PGC-1 in Epitrochlearis Muscle in Swimming-Exercised Rats |
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