Blue light regeneration of bacteriorhodopsin bleached by continuous light
Photobleaching of bacteriorhodopsin (BR) by continuous light has recently been demonstrated. This bleaching consists of at least two subsequent product states. One of them is absorbing maximally in the blue spectral region. Our present study shows that upon illumination of the bleached sample with b...
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| Veröffentlicht in: | FEBS letters 2000-07, Vol.476 (3), p.171-173 |
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| container_title | FEBS letters |
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| creator | Dancsházy, Zsolt Tokaji, Zsolt |
| description | Photobleaching of bacteriorhodopsin (BR) by continuous light has recently been demonstrated. This bleaching consists of at least two subsequent product states. One of them is absorbing maximally in the blue spectral region. Our present study shows that upon illumination of the bleached sample with blue light a back photoprocess appears, resulting in regeneration of the original BR state. From a technical point of view, the observed phenomenon is similar to the reverting effect of blue light on the photocycle. An important difference is that the photobleached state of BR is much more stable than any of the photocycle intermediates, and may provide an advantage for several technical applications. |
| doi_str_mv | 10.1016/S0014-5793(00)01714-2 |
| format | Article |
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This bleaching consists of at least two subsequent product states. One of them is absorbing maximally in the blue spectral region. Our present study shows that upon illumination of the bleached sample with blue light a back photoprocess appears, resulting in regeneration of the original BR state. From a technical point of view, the observed phenomenon is similar to the reverting effect of blue light on the photocycle. 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This bleaching consists of at least two subsequent product states. One of them is absorbing maximally in the blue spectral region. Our present study shows that upon illumination of the bleached sample with blue light a back photoprocess appears, resulting in regeneration of the original BR state. From a technical point of view, the observed phenomenon is similar to the reverting effect of blue light on the photocycle. An important difference is that the photobleached state of BR is much more stable than any of the photocycle intermediates, and may provide an advantage for several technical applications.</description><subject>Bacteriorhodopsins - chemistry</subject><subject>Bacteriorhodopsins - radiation effects</subject><subject>Blue light effect</subject><subject>Light</subject><subject>Photochemistry</subject><subject>Photoreaction</subject><subject>Spectrophotometry</subject><subject>Stability in vivo</subject><subject>Technical application of bacteriorhodopsin</subject><subject>Thermal denaturation</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkTFPwzAQhS0EoqXwE0CZEAwB20nqeEK0ammlSgzAbDn2tTVK42InoP57nKZCbDBZ5_ve3dM7hC4JviOYDO9fMCZpnDGe3GB8iwkLFT1CfZKzJE7SYX6M-j9ID515_45DnRN-inoEc5IMMeuj-ahsICrNal1HDlZQgZO1sVVkl1EhVQ3OWLe22m69qaKiBKnWoKNiFylb1aZqbOM7-Tk6WcrSw8XhHaC36eR1PIsXz0_z8eMiVlnCabyEgnBdQJppDUxrppMsZVLTBEDJ4CstOAmISjnFOlVSgeScBgC0lrxIBui6m7t19qMBX4uN8QrKUlYQzAhGKCM5zgOYdaBy1nsHS7F1ZiPdThAs2gzFPkPRBiQwFvsMBQ26q8OCptiA_qXqQgvArAO-TAm7_00V08mI7jtto71E-G53PXSjICT2acAJrwxUCrRxoGqhrfnD7Tcsvpaa</recordid><startdate>20000707</startdate><enddate>20000707</enddate><creator>Dancsházy, Zsolt</creator><creator>Tokaji, Zsolt</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000707</creationdate><title>Blue light regeneration of bacteriorhodopsin bleached by continuous light</title><author>Dancsházy, Zsolt ; Tokaji, Zsolt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5392-feb19dbe45dde7dd7d3547ad23eeca0914b91b19c4920d4cacea992ad2edda9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Bacteriorhodopsins - chemistry</topic><topic>Bacteriorhodopsins - radiation effects</topic><topic>Blue light effect</topic><topic>Light</topic><topic>Photochemistry</topic><topic>Photoreaction</topic><topic>Spectrophotometry</topic><topic>Stability in vivo</topic><topic>Technical application of bacteriorhodopsin</topic><topic>Thermal denaturation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dancsházy, Zsolt</creatorcontrib><creatorcontrib>Tokaji, Zsolt</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dancsházy, Zsolt</au><au>Tokaji, Zsolt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Blue light regeneration of bacteriorhodopsin bleached by continuous light</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-07-07</date><risdate>2000</risdate><volume>476</volume><issue>3</issue><spage>171</spage><epage>173</epage><pages>171-173</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Photobleaching of bacteriorhodopsin (BR) by continuous light has recently been demonstrated. This bleaching consists of at least two subsequent product states. One of them is absorbing maximally in the blue spectral region. Our present study shows that upon illumination of the bleached sample with blue light a back photoprocess appears, resulting in regeneration of the original BR state. From a technical point of view, the observed phenomenon is similar to the reverting effect of blue light on the photocycle. An important difference is that the photobleached state of BR is much more stable than any of the photocycle intermediates, and may provide an advantage for several technical applications.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10913607</pmid><doi>10.1016/S0014-5793(00)01714-2</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2000-07, Vol.476 (3), p.171-173 |
| issn | 0014-5793 1873-3468 |
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| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bacteriorhodopsins - chemistry Bacteriorhodopsins - radiation effects Blue light effect Light Photochemistry Photoreaction Spectrophotometry Stability in vivo Technical application of bacteriorhodopsin Thermal denaturation |
| title | Blue light regeneration of bacteriorhodopsin bleached by continuous light |
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