Sulfotransferases in a human colon carcinoma cell line, Caco-2
We investigated the expression of sulfotransferases (STs) in the human colon carcinoma cell line, Caco-2, which is widely used as a human intestine model for orally administered drugs. By reverse transcription-polymerase chain reaction (RT-PCR), we could detect mRNA expressions of phenol STs (P-ST),...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2000, Vol.23 (7), p.810-814 |
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| creator | SATOH, T MATSUI, M TAMURA, H.-O |
| description | We investigated the expression of sulfotransferases (STs) in the human colon carcinoma cell line, Caco-2, which is widely used as a human intestine model for orally administered drugs. By reverse transcription-polymerase chain reaction (RT-PCR), we could detect mRNA expressions of phenol STs (P-ST), hydroxysteroid ST (HS-ST) and estrogen ST (E-ST). Treatment with 1,25-dihydroxyvitamine D3, which induces CYP3A4 expression in Caco-2 cells, did not affect the ST expression of the cells. We detected significant P-ST activity toward 2-naphthol and dopamine in the cell extract. We also observed detectable HS-ST activity toward dehydroepiandrosterone (DHEA), but no E-ST activity toward beta-estradiol was observed in the extract. Analyses by RT-PCR and separation of P-ST activities on ion-exchange column chromatography showed that the thermolabile monoamine phenol sulfotransferase (M-PST) is dominant in Caco-2 cells. |
| doi_str_mv | 10.1248/bpb.23.810 |
| format | Article |
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By reverse transcription-polymerase chain reaction (RT-PCR), we could detect mRNA expressions of phenol STs (P-ST), hydroxysteroid ST (HS-ST) and estrogen ST (E-ST). Treatment with 1,25-dihydroxyvitamine D3, which induces CYP3A4 expression in Caco-2 cells, did not affect the ST expression of the cells. We detected significant P-ST activity toward 2-naphthol and dopamine in the cell extract. We also observed detectable HS-ST activity toward dehydroepiandrosterone (DHEA), but no E-ST activity toward beta-estradiol was observed in the extract. Analyses by RT-PCR and separation of P-ST activities on ion-exchange column chromatography showed that the thermolabile monoamine phenol sulfotransferase (M-PST) is dominant in Caco-2 cells.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.23.810</identifier><identifier>PMID: 10919357</identifier><language>eng</language><publisher>Tokyo: Maruzen</publisher><subject>Arylsulfotransferase - isolation & purification ; Biological and medical sciences ; Caco-2 Cells ; Chromatography, Ion Exchange ; Colon - cytology ; Colon - enzymology ; Colonic Neoplasms ; DEAE-Dextran - chemistry ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Humans ; Molecular and cellular biology ; Molecular genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sulfotransferases - biosynthesis</subject><ispartof>Biological & pharmaceutical bulletin, 2000, Vol.23 (7), p.810-814</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-aa6e437bf4453efaf2490f6d1f2e1ddc6e5a3601c0bdc8d079f2bfe87f107c443</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1481765$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10919357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SATOH, T</creatorcontrib><creatorcontrib>MATSUI, M</creatorcontrib><creatorcontrib>TAMURA, H.-O</creatorcontrib><title>Sulfotransferases in a human colon carcinoma cell line, Caco-2</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>We investigated the expression of sulfotransferases (STs) in the human colon carcinoma cell line, Caco-2, which is widely used as a human intestine model for orally administered drugs. By reverse transcription-polymerase chain reaction (RT-PCR), we could detect mRNA expressions of phenol STs (P-ST), hydroxysteroid ST (HS-ST) and estrogen ST (E-ST). Treatment with 1,25-dihydroxyvitamine D3, which induces CYP3A4 expression in Caco-2 cells, did not affect the ST expression of the cells. We detected significant P-ST activity toward 2-naphthol and dopamine in the cell extract. We also observed detectable HS-ST activity toward dehydroepiandrosterone (DHEA), but no E-ST activity toward beta-estradiol was observed in the extract. Analyses by RT-PCR and separation of P-ST activities on ion-exchange column chromatography showed that the thermolabile monoamine phenol sulfotransferase (M-PST) is dominant in Caco-2 cells.</description><subject>Arylsulfotransferase - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells</subject><subject>Chromatography, Ion Exchange</subject><subject>Colon - cytology</subject><subject>Colon - enzymology</subject><subject>Colonic Neoplasms</subject><subject>DEAE-Dextran - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sulfotransferases - biosynthesis</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1LxDAQhoMo7rp68QdID-JBbM0kaZNeBFn8ggUP6jlM0wQrbbMm24P_3iy7oJd5D_PwMvMQcg60ACbUbbNuCsYLBfSAzIELmZcMykMypzWovIJSzchJjF-UUkkZPyYzSJual3JO7t6m3vlNwDE6GzDamHVjhtnnNOCYGd_7NDGYbvQDZsb2fdZ3o73Jlmh8zk7JkcM-2rN9LsjH48P78jlfvT69LO9XuRESNjliZQWXjROi5NahY6KmrmrBMQttaypbIq8oGNq0RrVU1o41zirpgEojBF-Qq13vOvjvycaNHrq4vQZH66eoJTCZ_qwSeL0DTfAxBuv0OnQDhh8NVG9t6WRLM66TrQRf7FunZrDtP3SnJwGXewCjwd4lTaaLf5xQIKuS_wKKLXFj</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>SATOH, T</creator><creator>MATSUI, M</creator><creator>TAMURA, H.-O</creator><general>Maruzen</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2000</creationdate><title>Sulfotransferases in a human colon carcinoma cell line, Caco-2</title><author>SATOH, T ; MATSUI, M ; TAMURA, H.-O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-aa6e437bf4453efaf2490f6d1f2e1ddc6e5a3601c0bdc8d079f2bfe87f107c443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Arylsulfotransferase - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells</topic><topic>Chromatography, Ion Exchange</topic><topic>Colon - cytology</topic><topic>Colon - enzymology</topic><topic>Colonic Neoplasms</topic><topic>DEAE-Dextran - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sulfotransferases - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SATOH, T</creatorcontrib><creatorcontrib>MATSUI, M</creatorcontrib><creatorcontrib>TAMURA, H.-O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SATOH, T</au><au>MATSUI, M</au><au>TAMURA, H.-O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sulfotransferases in a human colon carcinoma cell line, Caco-2</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2000</date><risdate>2000</risdate><volume>23</volume><issue>7</issue><spage>810</spage><epage>814</epage><pages>810-814</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>We investigated the expression of sulfotransferases (STs) in the human colon carcinoma cell line, Caco-2, which is widely used as a human intestine model for orally administered drugs. By reverse transcription-polymerase chain reaction (RT-PCR), we could detect mRNA expressions of phenol STs (P-ST), hydroxysteroid ST (HS-ST) and estrogen ST (E-ST). Treatment with 1,25-dihydroxyvitamine D3, which induces CYP3A4 expression in Caco-2 cells, did not affect the ST expression of the cells. We detected significant P-ST activity toward 2-naphthol and dopamine in the cell extract. We also observed detectable HS-ST activity toward dehydroepiandrosterone (DHEA), but no E-ST activity toward beta-estradiol was observed in the extract. Analyses by RT-PCR and separation of P-ST activities on ion-exchange column chromatography showed that the thermolabile monoamine phenol sulfotransferase (M-PST) is dominant in Caco-2 cells.</abstract><cop>Tokyo</cop><pub>Maruzen</pub><pmid>10919357</pmid><doi>10.1248/bpb.23.810</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Arylsulfotransferase - isolation & purification Biological and medical sciences Caco-2 Cells Chromatography, Ion Exchange Colon - cytology Colon - enzymology Colonic Neoplasms DEAE-Dextran - chemistry Fundamental and applied biological sciences. Psychology Gene expression Humans Molecular and cellular biology Molecular genetics Reverse Transcriptase Polymerase Chain Reaction Sulfotransferases - biosynthesis |
| title | Sulfotransferases in a human colon carcinoma cell line, Caco-2 |
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