Intron‐tagged epitope: a tool for facile detection and purification of proteins expressed in Agrobacterium‐transformed plant cells

Summary Epitope tagging provides a useful tool for immunological detection and cellular localization of proteins in vivo. Using T‐DNA‐mediated transformation, the detection of epitope‐tagged proteins in planta is currently feasible only in transgenic plants, because an artificial expression of cDNA...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2000-06, Vol.22 (6), p.553-560
Hauptverfasser:	Ferrando, Alejandro, Farràs, Rosa, Jásik, Ján, Schell, Jeff, Koncz, Csaba
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins Biological and medical sciences Blotting, Western DNA, Bacterial - genetics Epitopes Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genes, Reporter Genetic Vectors Glucuronidase - genetics Glucuronidase - metabolism Introns Plant Proteins - analysis Plant Proteins - genetics Plant Proteins - metabolism Rhizobium - genetics Transformation, Genetic
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