Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species: Structure–activity relationship of cloned LPA receptors
We examined the structure–activity relationship of cloned lysophosphatidic acid (LPA) receptors (endothelial cell differentiation gene (EDG) 2, EDG4, and EDG7) by measuring [Ca 2+] i in Sf9 insect cells expressing each receptor using LPA with various acyl chains bound at either the sn-1 or the sn-2...
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| Veröffentlicht in: | FEBS letters 2000-07, Vol.478 (1), p.159-165 |
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| creator | Bandoh, Koji Aoki, Junken Taira, Akitsu Tsujimoto, Masafumi Arai, Hiroyuki Inoue, Keizo |
| description | We examined the structure–activity relationship of cloned lysophosphatidic acid (LPA) receptors (endothelial cell differentiation gene (EDG) 2, EDG4, and EDG7) by measuring [Ca
2+]
i in Sf9 insect cells expressing each receptor using LPA with various acyl chains bound at either the
sn-1 or the
sn-2 position of the glycerol backbone. For EDG7 the highest reactivity was observed with LPA with Δ9-unsaturated fatty acid (oleic (18:1), linoleic (18:2), and linolenic (18:3)) at
sn-2 followed by 2-palmitoleoyl (16:1) and 2-arachidonoyl (20:4) LPA. In contrast, EDG2 and EDG4 showed broad ligand specificities, although EDG2 and EDG4 discriminated between 14:0 (myristoyl) and 16:0 (palmitoyl), and 12:0 (lauroyl) and 14:0 LPAs, respectively. EDG7 recognizes the
cis double bond at the Δ9 position of octadecanoyl residues, since 2-elaidoyl (18:1,
trans) and 2-petroselinoyl (18:1,
cis-Δ12) LPA were poor ligands for EDG7. In conclusion, the present study demonstrates that each LPA receptor can be activated differentially by the LPA species. |
| doi_str_mv | 10.1016/S0014-5793(00)01827-5 |
| format | Article |
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2+]
i in Sf9 insect cells expressing each receptor using LPA with various acyl chains bound at either the
sn-1 or the
sn-2 position of the glycerol backbone. For EDG7 the highest reactivity was observed with LPA with Δ9-unsaturated fatty acid (oleic (18:1), linoleic (18:2), and linolenic (18:3)) at
sn-2 followed by 2-palmitoleoyl (16:1) and 2-arachidonoyl (20:4) LPA. In contrast, EDG2 and EDG4 showed broad ligand specificities, although EDG2 and EDG4 discriminated between 14:0 (myristoyl) and 16:0 (palmitoyl), and 12:0 (lauroyl) and 14:0 LPAs, respectively. EDG7 recognizes the
cis double bond at the Δ9 position of octadecanoyl residues, since 2-elaidoyl (18:1,
trans) and 2-petroselinoyl (18:1,
cis-Δ12) LPA were poor ligands for EDG7. In conclusion, the present study demonstrates that each LPA receptor can be activated differentially by the LPA species.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(00)01827-5</identifier><identifier>PMID: 10922489</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Calcium - metabolism ; Calcium Signaling - drug effects ; Cell Line ; Dose-Response Relationship, Drug ; EDG2 ; EDG4 ; EDG7 ; Endothelial cell differentiation gene family ; Humans ; Ligands ; Lysophosphatidic acid ; Lysophospholipids - chemistry ; Lysophospholipids - pharmacology ; Molecular Sequence Data ; Receptors, Cell Surface - chemistry ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Receptors, G-Protein-Coupled ; Receptors, Lysophosphatidic Acid ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Spodoptera ; Structure-Activity Relationship ; Substrate Specificity ; Transfection</subject><ispartof>FEBS letters, 2000-07, Vol.478 (1), p.159-165</ispartof><rights>2000 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c309t-3c73c52c3f9dd77d6e506e285a0a001ba8013257a78f63003b001d0e342fa37b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(00)01827-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10922489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bandoh, Koji</creatorcontrib><creatorcontrib>Aoki, Junken</creatorcontrib><creatorcontrib>Taira, Akitsu</creatorcontrib><creatorcontrib>Tsujimoto, Masafumi</creatorcontrib><creatorcontrib>Arai, Hiroyuki</creatorcontrib><creatorcontrib>Inoue, Keizo</creatorcontrib><title>Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species: Structure–activity relationship of cloned LPA receptors</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We examined the structure–activity relationship of cloned lysophosphatidic acid (LPA) receptors (endothelial cell differentiation gene (EDG) 2, EDG4, and EDG7) by measuring [Ca
2+]
i in Sf9 insect cells expressing each receptor using LPA with various acyl chains bound at either the
sn-1 or the
sn-2 position of the glycerol backbone. For EDG7 the highest reactivity was observed with LPA with Δ9-unsaturated fatty acid (oleic (18:1), linoleic (18:2), and linolenic (18:3)) at
sn-2 followed by 2-palmitoleoyl (16:1) and 2-arachidonoyl (20:4) LPA. In contrast, EDG2 and EDG4 showed broad ligand specificities, although EDG2 and EDG4 discriminated between 14:0 (myristoyl) and 16:0 (palmitoyl), and 12:0 (lauroyl) and 14:0 LPAs, respectively. EDG7 recognizes the
cis double bond at the Δ9 position of octadecanoyl residues, since 2-elaidoyl (18:1,
trans) and 2-petroselinoyl (18:1,
cis-Δ12) LPA were poor ligands for EDG7. In conclusion, the present study demonstrates that each LPA receptor can be activated differentially by the LPA species.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling - drug effects</subject><subject>Cell Line</subject><subject>Dose-Response Relationship, Drug</subject><subject>EDG2</subject><subject>EDG4</subject><subject>EDG7</subject><subject>Endothelial cell differentiation gene family</subject><subject>Humans</subject><subject>Ligands</subject><subject>Lysophosphatidic acid</subject><subject>Lysophospholipids - chemistry</subject><subject>Lysophospholipids - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Receptors, Cell Surface - chemistry</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Receptors, Lysophosphatidic Acid</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Spodoptera</subject><subject>Structure-Activity Relationship</subject><subject>Substrate Specificity</subject><subject>Transfection</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURS1ERYfCJ4C8Qu0i7XM8iRM2VVVKQRqpSIW15djPGqNMHGynUnb8A3s-ji_BmakqdqwsP537rt69hLxhcM6A1Rf3AGxdVKLlpwBnwJpSFNUzsmKN4AVf181zsnpCjsnLGL9D_jesfUGOGbRluW7aFfm9maMftz6OW5WccZoq7Qw93Xy5OqMBNY7Jh0i9pWmL9ObDLbVq5_qZqoDUOGsx4JCc6peRTu5BJTS0m2leQOOI2mF8T-9TmHSaAv75-WtPuTTn7X229EPcunEx0L0fsnYRPhm_IkdW9RFfP74n5NvHm6_Xn4rN3e3n66tNoTm0qeBacF2VmtvWGCFMjRXUWDaVApWP7lQDjJeVUKKxNQfgXZ4aQL4ureKi4yfk3WHvGPyPCWOSOxc19r0a0E9RClbWgteQweoA6uBjDGjlGNxOhVkykEsxcl-MXFKXAHJfjKyy7u2jwdTt0PyjOjSRgcsDgPnMB4dBxpzdoNG4nEaSxrv_WPwFAz-f1Q</recordid><startdate>20000728</startdate><enddate>20000728</enddate><creator>Bandoh, Koji</creator><creator>Aoki, Junken</creator><creator>Taira, Akitsu</creator><creator>Tsujimoto, Masafumi</creator><creator>Arai, Hiroyuki</creator><creator>Inoue, Keizo</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000728</creationdate><title>Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species: Structure–activity relationship of cloned LPA receptors</title><author>Bandoh, Koji ; Aoki, Junken ; Taira, Akitsu ; Tsujimoto, Masafumi ; Arai, Hiroyuki ; Inoue, Keizo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-3c73c52c3f9dd77d6e506e285a0a001ba8013257a78f63003b001d0e342fa37b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling - drug effects</topic><topic>Cell Line</topic><topic>Dose-Response Relationship, Drug</topic><topic>EDG2</topic><topic>EDG4</topic><topic>EDG7</topic><topic>Endothelial cell differentiation gene family</topic><topic>Humans</topic><topic>Ligands</topic><topic>Lysophosphatidic acid</topic><topic>Lysophospholipids - chemistry</topic><topic>Lysophospholipids - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Receptors, Cell Surface - chemistry</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, G-Protein-Coupled</topic><topic>Receptors, Lysophosphatidic Acid</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Spodoptera</topic><topic>Structure-Activity Relationship</topic><topic>Substrate Specificity</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bandoh, Koji</creatorcontrib><creatorcontrib>Aoki, Junken</creatorcontrib><creatorcontrib>Taira, Akitsu</creatorcontrib><creatorcontrib>Tsujimoto, Masafumi</creatorcontrib><creatorcontrib>Arai, Hiroyuki</creatorcontrib><creatorcontrib>Inoue, Keizo</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bandoh, Koji</au><au>Aoki, Junken</au><au>Taira, Akitsu</au><au>Tsujimoto, Masafumi</au><au>Arai, Hiroyuki</au><au>Inoue, Keizo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species: Structure–activity relationship of cloned LPA receptors</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-07-28</date><risdate>2000</risdate><volume>478</volume><issue>1</issue><spage>159</spage><epage>165</epage><pages>159-165</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>We examined the structure–activity relationship of cloned lysophosphatidic acid (LPA) receptors (endothelial cell differentiation gene (EDG) 2, EDG4, and EDG7) by measuring [Ca
2+]
i in Sf9 insect cells expressing each receptor using LPA with various acyl chains bound at either the
sn-1 or the
sn-2 position of the glycerol backbone. For EDG7 the highest reactivity was observed with LPA with Δ9-unsaturated fatty acid (oleic (18:1), linoleic (18:2), and linolenic (18:3)) at
sn-2 followed by 2-palmitoleoyl (16:1) and 2-arachidonoyl (20:4) LPA. In contrast, EDG2 and EDG4 showed broad ligand specificities, although EDG2 and EDG4 discriminated between 14:0 (myristoyl) and 16:0 (palmitoyl), and 12:0 (lauroyl) and 14:0 LPAs, respectively. EDG7 recognizes the
cis double bond at the Δ9 position of octadecanoyl residues, since 2-elaidoyl (18:1,
trans) and 2-petroselinoyl (18:1,
cis-Δ12) LPA were poor ligands for EDG7. In conclusion, the present study demonstrates that each LPA receptor can be activated differentially by the LPA species.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10922489</pmid><doi>10.1016/S0014-5793(00)01827-5</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2000-07, Vol.478 (1), p.159-165 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71267360 |
| source | Electronic Journals Library; MEDLINE; Wiley Journals; Elsevier ScienceDirect Journals Complete; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Calcium - metabolism Calcium Signaling - drug effects Cell Line Dose-Response Relationship, Drug EDG2 EDG4 EDG7 Endothelial cell differentiation gene family Humans Ligands Lysophosphatidic acid Lysophospholipids - chemistry Lysophospholipids - pharmacology Molecular Sequence Data Receptors, Cell Surface - chemistry Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Receptors, G-Protein-Coupled Receptors, Lysophosphatidic Acid Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Spodoptera Structure-Activity Relationship Substrate Specificity Transfection |
| title | Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species: Structure–activity relationship of cloned LPA receptors |
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