A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana
We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination...
Gespeichert in:
| Veröffentlicht in: | Plant and cell physiology 2000-05, Vol.41 (5), p.541-547 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 547 |
|---|---|
| container_issue | 5 |
| container_start_page | 541 |
| container_title | Plant and cell physiology |
| container_volume | 41 |
| creator | Lopez-Molina, L. (Rockefeller Univ., New York (USA)) Chua, N.H |
| description | We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination-insensitivity. Here we present the isolation of the gia1 mutant as well as the identification of the gia1 gene by positional cloning and complementation studies. GIA1 is predicted to code for a bZIP transcription factor with high homology to previously characterized plant bZIP transcription factors (DPBF1, ABFs and TRAB1) known for their ability to bind ABA-responsive DNA elements. Our results provide in vivo evidence that a bZIP factor may indeed be involved in ABA signaling. Since GIA1 turned out to be identical to ABI5, we designated GIA1 as ABI5 in the present paper. |
| doi_str_mv | 10.1093/pcp/41.5.541 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71264306</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17559727</sourcerecordid><originalsourceid>FETCH-LOGICAL-c507t-3317c39697d269db9b66e4c6c7b3a9540ffed342aa03a5dc38c8068f33179af53</originalsourceid><addsrcrecordid>eNqF0E1P3DAQBmCralUW6I0rlU89NYsdf8XHFLUFhARatQVxsSaODW6zSWo7qPz7ZhWEeutcZqR5Zg4vQkeUrCnR7GS04wmna7EWnL5CK8oVLTQR7DVaEcLKgqiK7qH9lH4SMs-MvEV782GpNZMrtKlxP3Ud3k4Zchh6HHoMuLk7v8YebB4itkPvXUy4_lQXoU-uTyGHx5CfdrSO0IR2GFNIOD9AF6CHQ_TGQ5fcu-d-gL5_-fzt9Ky4vPp6flpfFlYQlQvGqLJMS63aUuq20Y2UjltpVcNAC068dy3jJQBhIFrLKlsRWfndnQYv2AH6sPwd4_B7cimbbUjWdR30bpiSUbSUnBH5X0iVEFqVaoYfF2jjkFJ03owxbCE-GUrMLmwzh204NcLMYc_8_fPfqdm69h-8pDuDYgEhZffnZQ_xl5GKKWHObu_Mj01FNzcXN2Yz--PFexgM3MeQzMV1SXYlOdfsLxTUkas</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17559727</pqid></control><display><type>article</type><title>A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana</title><source>Freely Accessible Japanese Titles - check A-Z of ejournals</source><source>MEDLINE</source><source>EZB Electronic Journals Library</source><source>Oxford Journals</source><creator>Lopez-Molina, L. (Rockefeller Univ., New York (USA)) ; Chua, N.H</creator><creatorcontrib>Lopez-Molina, L. (Rockefeller Univ., New York (USA)) ; Chua, N.H</creatorcontrib><description>We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination-insensitivity. Here we present the isolation of the gia1 mutant as well as the identification of the gia1 gene by positional cloning and complementation studies. GIA1 is predicted to code for a bZIP transcription factor with high homology to previously characterized plant bZIP transcription factors (DPBF1, ABFs and TRAB1) known for their ability to bind ABA-responsive DNA elements. Our results provide in vivo evidence that a bZIP factor may indeed be involved in ABA signaling. Since GIA1 turned out to be identical to ABI5, we designated GIA1 as ABI5 in the present paper.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/41.5.541</identifier><identifier>PMID: 10929936</identifier><language>eng</language><publisher>Japan: Plant and Cell Physiology</publisher><subject>ABA ; ABI5 gene ; Abscisic acid ; Abscisic Acid - pharmacology ; Amino Acid Sequence ; Arabidopsis ; Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - physiology ; ARABIDOPSIS THALIANA ; BIOCHEMICAL PATHWAYS ; Chromosome Mapping ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Conserved Sequence ; GENE ; GENES ; Genetic Complementation Test ; Genetics ; GIA1 gene ; Leucine Zippers ; MOLECULAR CLONING ; Molecular Sequence Data ; MUTANT ; MUTANTES ; MUTANTS ; Plant Growth Regulators - pharmacology ; Plant Proteins - genetics ; Polymerase Chain Reaction ; Positional cloning ; RESISTANCE AUX PRODUITS CHIMIQUES ; RESISTANCE TO CHEMICALS ; RESISTENCIA A PRODUCTOS QUIMICOS ; Sequence Alignment ; Sequence Homology, Amino Acid ; Signal Transduction - drug effects ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription Factors - genetics ; VIA BIOQUIMICA DEL METABOLISMO ; VOIE BIOCHIMIQUE DU METABOLISME</subject><ispartof>Plant and cell physiology, 2000-05, Vol.41 (5), p.541-547</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-3317c39697d269db9b66e4c6c7b3a9540ffed342aa03a5dc38c8068f33179af53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10929936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopez-Molina, L. (Rockefeller Univ., New York (USA))</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><title>A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination-insensitivity. Here we present the isolation of the gia1 mutant as well as the identification of the gia1 gene by positional cloning and complementation studies. GIA1 is predicted to code for a bZIP transcription factor with high homology to previously characterized plant bZIP transcription factors (DPBF1, ABFs and TRAB1) known for their ability to bind ABA-responsive DNA elements. Our results provide in vivo evidence that a bZIP factor may indeed be involved in ABA signaling. Since GIA1 turned out to be identical to ABI5, we designated GIA1 as ABI5 in the present paper.</description><subject>ABA</subject><subject>ABI5 gene</subject><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>ARABIDOPSIS THALIANA</subject><subject>BIOCHEMICAL PATHWAYS</subject><subject>Chromosome Mapping</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Conserved Sequence</subject><subject>GENE</subject><subject>GENES</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>GIA1 gene</subject><subject>Leucine Zippers</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Plant Growth Regulators - pharmacology</subject><subject>Plant Proteins - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Positional cloning</subject><subject>RESISTANCE AUX PRODUITS CHIMIQUES</subject><subject>RESISTANCE TO CHEMICALS</subject><subject>RESISTENCIA A PRODUCTOS QUIMICOS</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction - drug effects</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription Factors - genetics</subject><subject>VIA BIOQUIMICA DEL METABOLISMO</subject><subject>VOIE BIOCHIMIQUE DU METABOLISME</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1P3DAQBmCralUW6I0rlU89NYsdf8XHFLUFhARatQVxsSaODW6zSWo7qPz7ZhWEeutcZqR5Zg4vQkeUrCnR7GS04wmna7EWnL5CK8oVLTQR7DVaEcLKgqiK7qH9lH4SMs-MvEV782GpNZMrtKlxP3Ud3k4Zchh6HHoMuLk7v8YebB4itkPvXUy4_lQXoU-uTyGHx5CfdrSO0IR2GFNIOD9AF6CHQ_TGQ5fcu-d-gL5_-fzt9Ky4vPp6flpfFlYQlQvGqLJMS63aUuq20Y2UjltpVcNAC068dy3jJQBhIFrLKlsRWfndnQYv2AH6sPwd4_B7cimbbUjWdR30bpiSUbSUnBH5X0iVEFqVaoYfF2jjkFJ03owxbCE-GUrMLmwzh204NcLMYc_8_fPfqdm69h-8pDuDYgEhZffnZQ_xl5GKKWHObu_Mj01FNzcXN2Yz--PFexgM3MeQzMV1SXYlOdfsLxTUkas</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Lopez-Molina, L. (Rockefeller Univ., New York (USA))</creator><creator>Chua, N.H</creator><general>Plant and Cell Physiology</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana</title><author>Lopez-Molina, L. (Rockefeller Univ., New York (USA)) ; Chua, N.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-3317c39697d269db9b66e4c6c7b3a9540ffed342aa03a5dc38c8068f33179af53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>ABA</topic><topic>ABI5 gene</topic><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>ARABIDOPSIS THALIANA</topic><topic>BIOCHEMICAL PATHWAYS</topic><topic>Chromosome Mapping</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Conserved Sequence</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>GIA1 gene</topic><topic>Leucine Zippers</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant Proteins - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Positional cloning</topic><topic>RESISTANCE AUX PRODUITS CHIMIQUES</topic><topic>RESISTANCE TO CHEMICALS</topic><topic>RESISTENCIA A PRODUCTOS QUIMICOS</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction - drug effects</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription Factors - genetics</topic><topic>VIA BIOQUIMICA DEL METABOLISMO</topic><topic>VOIE BIOCHIMIQUE DU METABOLISME</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopez-Molina, L. (Rockefeller Univ., New York (USA))</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopez-Molina, L. (Rockefeller Univ., New York (USA))</au><au>Chua, N.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>41</volume><issue>5</issue><spage>541</spage><epage>547</epage><pages>541-547</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>We have used a modification of the classical ABA-insensitive screen (Koornneef et al. 1984) to isolate novel mutations in the ABA signal transduction pathway of Arabidopsis thaliana. In our screen, mutants were recovered on the basis of their growth-insensitivity to ABA (GIA) rather than germination-insensitivity. Here we present the isolation of the gia1 mutant as well as the identification of the gia1 gene by positional cloning and complementation studies. GIA1 is predicted to code for a bZIP transcription factor with high homology to previously characterized plant bZIP transcription factors (DPBF1, ABFs and TRAB1) known for their ability to bind ABA-responsive DNA elements. Our results provide in vivo evidence that a bZIP factor may indeed be involved in ABA signaling. Since GIA1 turned out to be identical to ABI5, we designated GIA1 as ABI5 in the present paper.</abstract><cop>Japan</cop><pub>Plant and Cell Physiology</pub><pmid>10929936</pmid><doi>10.1093/pcp/41.5.541</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0781 |
| ispartof | Plant and cell physiology, 2000-05, Vol.41 (5), p.541-547 |
| issn | 0032-0781 1471-9053 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71264306 |
| source | Freely Accessible Japanese Titles - check A-Z of ejournals; MEDLINE; EZB Electronic Journals Library; Oxford Journals |
| subjects | ABA ABI5 gene Abscisic acid Abscisic Acid - pharmacology Amino Acid Sequence Arabidopsis Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - physiology ARABIDOPSIS THALIANA BIOCHEMICAL PATHWAYS Chromosome Mapping CLONACION MOLECULAR CLONAGE MOLECULAIRE Conserved Sequence GENE GENES Genetic Complementation Test Genetics GIA1 gene Leucine Zippers MOLECULAR CLONING Molecular Sequence Data MUTANT MUTANTES MUTANTS Plant Growth Regulators - pharmacology Plant Proteins - genetics Polymerase Chain Reaction Positional cloning RESISTANCE AUX PRODUITS CHIMIQUES RESISTANCE TO CHEMICALS RESISTENCIA A PRODUCTOS QUIMICOS Sequence Alignment Sequence Homology, Amino Acid Signal Transduction - drug effects TRANSCRIPCION TRANSCRIPTION Transcription Factors - genetics VIA BIOQUIMICA DEL METABOLISMO VOIE BIOCHIMIQUE DU METABOLISME |
| title | A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T18%3A34%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20null%20mutation%20in%20a%20bZIP%20factor%20confers%20ABA-insensitivity%20in%20Arabidopsis%20thaliana&rft.jtitle=Plant%20and%20cell%20physiology&rft.au=Lopez-Molina,%20L.%20(Rockefeller%20Univ.,%20New%20York%20(USA))&rft.date=2000-05-01&rft.volume=41&rft.issue=5&rft.spage=541&rft.epage=547&rft.pages=541-547&rft.issn=0032-0781&rft.eissn=1471-9053&rft_id=info:doi/10.1093/pcp/41.5.541&rft_dat=%3Cproquest_cross%3E17559727%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17559727&rft_id=info:pmid/10929936&rfr_iscdi=true |