The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells
Cochlear outer hair cells (OHCs) possess a unique fast voltage‐driven motility associated with a voltage‐sensitive motor protein embedded in the basolateral membrane. This mechanism is believed to underlie the cochlear amplification in mammals. OHCs also have a Ca2+/calmodulin‐dependent mechanical p...
Gespeichert in:
| Veröffentlicht in: | The European journal of neuroscience 2001-12, Vol.14 (12), p.1947-1952 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1952 |
|---|---|
| container_issue | 12 |
| container_start_page | 1947 |
| container_title | The European journal of neuroscience |
| container_volume | 14 |
| creator | Beurg, Maryline Bouleau, Yohan Dulon, Didier |
| description | Cochlear outer hair cells (OHCs) possess a unique fast voltage‐driven motility associated with a voltage‐sensitive motor protein embedded in the basolateral membrane. This mechanism is believed to underlie the cochlear amplification in mammals. OHCs also have a Ca2+/calmodulin‐dependent mechanical pathway which involves a submembranous circumferential cytoskeleton. The purpose of this study was to compare the functional appearance of the voltage‐sensitive motor proteins with that involving the Ca2+‐sensitive cytoskeleton during postnatal development of rat OHCs. We demonstrate that whole‐cell electromotility and Ca2+‐voked mechanical responses, by ionomycin, develop concomitantly after postnatal day 5 (P5). These two mechanical properties also develop simultaneously in OHCs isolated from two‐week‐old cultures of P0‐P1 organs of Corti. This excludes the participation of neural innervation in the postnatal maturation of the OHCs' motile properties. In addition, we show that the expression of the membranous voltage‐sensitive motor protein precedes, by several days, the appearance of whole‐cell electromotility. The concomitant development of whole‐cell electromotility and Ca2+‐sensitive motility, both in vivo and in vitro, underlines the cytoskeleton as an important factor in the functional organization of the voltage‐sensitive motor proteins within the plasma membrane. |
| doi_str_mv | 10.1046/j.0953-816x.2001.01826.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71261889</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71261889</sourcerecordid><originalsourceid>FETCH-LOGICAL-i2316-5c50fa92547c8a570c462276f0e24d582df7d5b9febf83f3e41f8efcd62120c23</originalsourceid><addsrcrecordid>eNpNkU1PGzEQhi1UVFLav1D5xAXtYnvXXu-hBxTxKUpbiRbExXK8Y-KwWQfbSZN_z27DR08z0jzvaOZ9EcKU5JSU4miWk5oXmaRinTNCaE6oZCJf76ARLQXJai7kBzR6he720KcYZ4QQKUr-Ee1RKgUpZT1C8WYKeOXbpB8gi9BFl9wK8NwnH_Ai-ASuw7prcOq5sWaH_0Fmk3x8hBaS73CPNbCC1i9c94CDTth4M21BB-yXCQKeahewgbaNn9Gu1W2ELy91H_0-PbkZn2dXP84uxsdXmWMFFRk3nFhdM15WRmpeEVMKxiphCbCy4ZI1tmr4pLYwsbKwBZTUSrCmEYwyYlixjw62e_s_npYQk5q7OFygO_DLqCrKBJWy7sGvL-ByModGLYKb67BRrzb1wLct8Ne1sHmfEzXEoWZqcFoNcaghDvUvDrVWJ5fXQ9frs63exQTrN70Oj0pURcXV7fWZuvte3P68_8XUn-IZH-iPpQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71261889</pqid></control><display><type>article</type><title>The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Beurg, Maryline ; Bouleau, Yohan ; Dulon, Didier</creator><creatorcontrib>Beurg, Maryline ; Bouleau, Yohan ; Dulon, Didier</creatorcontrib><description>Cochlear outer hair cells (OHCs) possess a unique fast voltage‐driven motility associated with a voltage‐sensitive motor protein embedded in the basolateral membrane. This mechanism is believed to underlie the cochlear amplification in mammals. OHCs also have a Ca2+/calmodulin‐dependent mechanical pathway which involves a submembranous circumferential cytoskeleton. The purpose of this study was to compare the functional appearance of the voltage‐sensitive motor proteins with that involving the Ca2+‐sensitive cytoskeleton during postnatal development of rat OHCs. We demonstrate that whole‐cell electromotility and Ca2+‐voked mechanical responses, by ionomycin, develop concomitantly after postnatal day 5 (P5). These two mechanical properties also develop simultaneously in OHCs isolated from two‐week‐old cultures of P0‐P1 organs of Corti. This excludes the participation of neural innervation in the postnatal maturation of the OHCs' motile properties. In addition, we show that the expression of the membranous voltage‐sensitive motor protein precedes, by several days, the appearance of whole‐cell electromotility. The concomitant development of whole‐cell electromotility and Ca2+‐sensitive motility, both in vivo and in vitro, underlines the cytoskeleton as an important factor in the functional organization of the voltage‐sensitive motor proteins within the plasma membrane.</description><identifier>ISSN: 0953-816X</identifier><identifier>EISSN: 1460-9568</identifier><identifier>DOI: 10.1046/j.0953-816x.2001.01826.x</identifier><identifier>PMID: 11860489</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Aging - metabolism ; Animals ; Animals, Newborn ; Calcium Signaling - drug effects ; Calcium Signaling - physiology ; Cell Differentiation - physiology ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Movement - drug effects ; Cell Movement - physiology ; cochlea ; Cytoskeleton - drug effects ; Cytoskeleton - metabolism ; Electric Stimulation ; electromotility ; Hair Cells, Auditory, Outer - cytology ; Hair Cells, Auditory, Outer - growth & development ; Hair Cells, Auditory, Outer - metabolism ; Hearing - physiology ; Ion Channels - drug effects ; Ion Channels - metabolism ; ionomycin ; Ionomycin - pharmacology ; Ionophores - pharmacology ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Molecular Motor Proteins - drug effects ; Molecular Motor Proteins - metabolism ; motor protein ; Organ Culture Techniques ; prestin ; Rats ; Rats, Wistar ; Signal Transduction - drug effects ; Signal Transduction - physiology</subject><ispartof>The European journal of neuroscience, 2001-12, Vol.14 (12), p.1947-1952</ispartof><rights>Federation of European Neuroscience Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.0953-816x.2001.01826.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.0953-816x.2001.01826.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11860489$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beurg, Maryline</creatorcontrib><creatorcontrib>Bouleau, Yohan</creatorcontrib><creatorcontrib>Dulon, Didier</creatorcontrib><title>The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells</title><title>The European journal of neuroscience</title><addtitle>Eur J Neurosci</addtitle><description>Cochlear outer hair cells (OHCs) possess a unique fast voltage‐driven motility associated with a voltage‐sensitive motor protein embedded in the basolateral membrane. This mechanism is believed to underlie the cochlear amplification in mammals. OHCs also have a Ca2+/calmodulin‐dependent mechanical pathway which involves a submembranous circumferential cytoskeleton. The purpose of this study was to compare the functional appearance of the voltage‐sensitive motor proteins with that involving the Ca2+‐sensitive cytoskeleton during postnatal development of rat OHCs. We demonstrate that whole‐cell electromotility and Ca2+‐voked mechanical responses, by ionomycin, develop concomitantly after postnatal day 5 (P5). These two mechanical properties also develop simultaneously in OHCs isolated from two‐week‐old cultures of P0‐P1 organs of Corti. This excludes the participation of neural innervation in the postnatal maturation of the OHCs' motile properties. In addition, we show that the expression of the membranous voltage‐sensitive motor protein precedes, by several days, the appearance of whole‐cell electromotility. The concomitant development of whole‐cell electromotility and Ca2+‐sensitive motility, both in vivo and in vitro, underlines the cytoskeleton as an important factor in the functional organization of the voltage‐sensitive motor proteins within the plasma membrane.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Calcium Signaling - drug effects</subject><subject>Calcium Signaling - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - physiology</subject><subject>cochlea</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - metabolism</subject><subject>Electric Stimulation</subject><subject>electromotility</subject><subject>Hair Cells, Auditory, Outer - cytology</subject><subject>Hair Cells, Auditory, Outer - growth & development</subject><subject>Hair Cells, Auditory, Outer - metabolism</subject><subject>Hearing - physiology</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - metabolism</subject><subject>ionomycin</subject><subject>Ionomycin - pharmacology</subject><subject>Ionophores - pharmacology</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Molecular Motor Proteins - drug effects</subject><subject>Molecular Motor Proteins - metabolism</subject><subject>motor protein</subject><subject>Organ Culture Techniques</subject><subject>prestin</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU1PGzEQhi1UVFLav1D5xAXtYnvXXu-hBxTxKUpbiRbExXK8Y-KwWQfbSZN_z27DR08z0jzvaOZ9EcKU5JSU4miWk5oXmaRinTNCaE6oZCJf76ARLQXJai7kBzR6he720KcYZ4QQKUr-Ee1RKgUpZT1C8WYKeOXbpB8gi9BFl9wK8NwnH_Ai-ASuw7prcOq5sWaH_0Fmk3x8hBaS73CPNbCC1i9c94CDTth4M21BB-yXCQKeahewgbaNn9Gu1W2ELy91H_0-PbkZn2dXP84uxsdXmWMFFRk3nFhdM15WRmpeEVMKxiphCbCy4ZI1tmr4pLYwsbKwBZTUSrCmEYwyYlixjw62e_s_npYQk5q7OFygO_DLqCrKBJWy7sGvL-ByModGLYKb67BRrzb1wLct8Ne1sHmfEzXEoWZqcFoNcaghDvUvDrVWJ5fXQ9frs63exQTrN70Oj0pURcXV7fWZuvte3P68_8XUn-IZH-iPpQ</recordid><startdate>200112</startdate><enddate>200112</enddate><creator>Beurg, Maryline</creator><creator>Bouleau, Yohan</creator><creator>Dulon, Didier</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200112</creationdate><title>The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells</title><author>Beurg, Maryline ; Bouleau, Yohan ; Dulon, Didier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2316-5c50fa92547c8a570c462276f0e24d582df7d5b9febf83f3e41f8efcd62120c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Calcium Signaling - drug effects</topic><topic>Calcium Signaling - physiology</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - physiology</topic><topic>cochlea</topic><topic>Cytoskeleton - drug effects</topic><topic>Cytoskeleton - metabolism</topic><topic>Electric Stimulation</topic><topic>electromotility</topic><topic>Hair Cells, Auditory, Outer - cytology</topic><topic>Hair Cells, Auditory, Outer - growth & development</topic><topic>Hair Cells, Auditory, Outer - metabolism</topic><topic>Hearing - physiology</topic><topic>Ion Channels - drug effects</topic><topic>Ion Channels - metabolism</topic><topic>ionomycin</topic><topic>Ionomycin - pharmacology</topic><topic>Ionophores - pharmacology</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Molecular Motor Proteins - drug effects</topic><topic>Molecular Motor Proteins - metabolism</topic><topic>motor protein</topic><topic>Organ Culture Techniques</topic><topic>prestin</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beurg, Maryline</creatorcontrib><creatorcontrib>Bouleau, Yohan</creatorcontrib><creatorcontrib>Dulon, Didier</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beurg, Maryline</au><au>Bouleau, Yohan</au><au>Dulon, Didier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>2001-12</date><risdate>2001</risdate><volume>14</volume><issue>12</issue><spage>1947</spage><epage>1952</epage><pages>1947-1952</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>Cochlear outer hair cells (OHCs) possess a unique fast voltage‐driven motility associated with a voltage‐sensitive motor protein embedded in the basolateral membrane. This mechanism is believed to underlie the cochlear amplification in mammals. OHCs also have a Ca2+/calmodulin‐dependent mechanical pathway which involves a submembranous circumferential cytoskeleton. The purpose of this study was to compare the functional appearance of the voltage‐sensitive motor proteins with that involving the Ca2+‐sensitive cytoskeleton during postnatal development of rat OHCs. We demonstrate that whole‐cell electromotility and Ca2+‐voked mechanical responses, by ionomycin, develop concomitantly after postnatal day 5 (P5). These two mechanical properties also develop simultaneously in OHCs isolated from two‐week‐old cultures of P0‐P1 organs of Corti. This excludes the participation of neural innervation in the postnatal maturation of the OHCs' motile properties. In addition, we show that the expression of the membranous voltage‐sensitive motor protein precedes, by several days, the appearance of whole‐cell electromotility. The concomitant development of whole‐cell electromotility and Ca2+‐sensitive motility, both in vivo and in vitro, underlines the cytoskeleton as an important factor in the functional organization of the voltage‐sensitive motor proteins within the plasma membrane.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>11860489</pmid><doi>10.1046/j.0953-816x.2001.01826.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0953-816X |
| ispartof | The European journal of neuroscience, 2001-12, Vol.14 (12), p.1947-1952 |
| issn | 0953-816X 1460-9568 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71261889 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Aging - metabolism Animals Animals, Newborn Calcium Signaling - drug effects Calcium Signaling - physiology Cell Differentiation - physiology Cell Membrane - drug effects Cell Membrane - metabolism Cell Movement - drug effects Cell Movement - physiology cochlea Cytoskeleton - drug effects Cytoskeleton - metabolism Electric Stimulation electromotility Hair Cells, Auditory, Outer - cytology Hair Cells, Auditory, Outer - growth & development Hair Cells, Auditory, Outer - metabolism Hearing - physiology Ion Channels - drug effects Ion Channels - metabolism ionomycin Ionomycin - pharmacology Ionophores - pharmacology Membrane Potentials - drug effects Membrane Potentials - physiology Molecular Motor Proteins - drug effects Molecular Motor Proteins - metabolism motor protein Organ Culture Techniques prestin Rats Rats, Wistar Signal Transduction - drug effects Signal Transduction - physiology |
| title | The voltage-sensitive motor protein and the Ca2+-sensitive cytoskeleton in developing rat cochlear outer hair cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T15%3A18%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20voltage-sensitive%20motor%20protein%20and%20the%20Ca2+-sensitive%20cytoskeleton%20in%20developing%20rat%20cochlear%20outer%20hair%20cells&rft.jtitle=The%20European%20journal%20of%20neuroscience&rft.au=Beurg,%20Maryline&rft.date=2001-12&rft.volume=14&rft.issue=12&rft.spage=1947&rft.epage=1952&rft.pages=1947-1952&rft.issn=0953-816X&rft.eissn=1460-9568&rft_id=info:doi/10.1046/j.0953-816x.2001.01826.x&rft_dat=%3Cproquest_pubme%3E71261889%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71261889&rft_id=info:pmid/11860489&rfr_iscdi=true |