The use of competitive PCR mimic to evaluate a Limulus lambda phage genomic DNA library

1. A lambda phage genomic DNA library for Limulus (L.) polyphemus brain was constructed using the AGEM-12 vector and the host strain KW251. 2. The primary library contained approximately 1.275 x 10(6) independent clones, increasing upon amplfication to 6.66 x 10(9) pfu/ml in a total volume of 58 ml....

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Veröffentlicht in:	Cellular and molecular neurobiology 2000-08, Vol.20 (4), p.509-520
Hauptverfasser:	Wang, Y, Cao, Z, Reid, E A, Newkirk, R F, Ivy, M T, Townsel, J G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriophage lambda - genetics Brain Chemistry - genetics DNA - analysis DNA libraries Genomic Library Horseshoe Crabs - genetics Horseshoe Crabs - metabolism Lambda Phage Limulus polyphemus Polymerase Chain Reaction
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