A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline
Results of a first successful application of a direct photo-induced affinity modification of Tet repressor (TetR(D)) protein with tetracycline within a complex of known three-dimensional structure are described. The conditions of the modification have provided suitable yields of the modified complex...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 2000-07, Vol.477 (3), p.263-267 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 267 |
|---|---|
| container_issue | 3 |
| container_start_page | 263 |
| container_title | FEBS letters |
| container_volume | 477 |
| creator | Beliakova, Maria M. Anokhina, Maria M. Spiridonova, Vera A. Dobrov, Eugeny N. Egorov, Tsezi A. Wittmann-Liebold, Brigitte Orth, Peter Saenger, Wolfram Kopylov, Alexei M. |
| description | Results of a first successful application of a direct photo-induced affinity modification of Tet repressor (TetR(D)) protein with tetracycline within a complex of known three-dimensional structure are described. The conditions of the modification have provided suitable yields of the modified complex and allowed characterization of the modified segments of the protein. The potential of tetracycline as a fine modifying reagent was established. In the complex of TetR(D) protein with tetracycline, the antibiotic modifies at least two segments, Ile59–Glu73 and Ala173–Glu183, which form a binding tunnel for the drug according to the X-ray analysis. These data open possibilities for the use of different tetracycline targets for structural studies in solution. |
| doi_str_mv | 10.1016/S0014-5793(00)01728-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71256331</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579300017282</els_id><sourcerecordid>71256331</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4262-267c8665bae652911ef45d2d0c5f79adfe9f01a58642f960e62ee6d91c355ad03</originalsourceid><addsrcrecordid>eNqNkUtv1TAQhS0EopfCTwB5hdpFqO3ETrJCpQ9aqRISlLXl2mN1UG4cbN-W--_r3FSoXcHKjznnzOgbQt5z9okzro5-MMabSrZ9fcDYIeOt6Crxgqx419ZV3ajuJVn9leyRNyn9YuXd8f412eOsZ0UnVuTPMXUYwWY63YYcKmMz3pkMjhrvccS8pevg0KM1GcNIg6cZcjR2awccgZbrmGzEaVeNMEVIKUQ6xZABR3oN-fvB6SG9x3z7zPmWvPJmSPDu8dwnP8_Prk8uqqtvXy9Pjq8q2wglKqFa2yklbwwoKXrOwTfSCces9G1vnIfeM25kpxrhe8VACQDlem5rKY1j9T75uOSWiX5vIGW9xmRhGMwIYZN0y4VUdc2LUC5CG0NKEbyeIq5N3GrO9Ixc75DrmadmTO-Qa1F8Hx4bbG7W4J64FsZFcLEI7nGA7f-l6vOzL2JXmQvz4sr3HPV5iYJC7A4h6mQRRgvLDrUL-I9pHwDMgKbN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71256331</pqid></control><display><type>article</type><title>A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Wiley Online Library Free Content</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Beliakova, Maria M. ; Anokhina, Maria M. ; Spiridonova, Vera A. ; Dobrov, Eugeny N. ; Egorov, Tsezi A. ; Wittmann-Liebold, Brigitte ; Orth, Peter ; Saenger, Wolfram ; Kopylov, Alexei M.</creator><creatorcontrib>Beliakova, Maria M. ; Anokhina, Maria M. ; Spiridonova, Vera A. ; Dobrov, Eugeny N. ; Egorov, Tsezi A. ; Wittmann-Liebold, Brigitte ; Orth, Peter ; Saenger, Wolfram ; Kopylov, Alexei M.</creatorcontrib><description>Results of a first successful application of a direct photo-induced affinity modification of Tet repressor (TetR(D)) protein with tetracycline within a complex of known three-dimensional structure are described. The conditions of the modification have provided suitable yields of the modified complex and allowed characterization of the modified segments of the protein. The potential of tetracycline as a fine modifying reagent was established. In the complex of TetR(D) protein with tetracycline, the antibiotic modifies at least two segments, Ile59–Glu73 and Ala173–Glu183, which form a binding tunnel for the drug according to the X-ray analysis. These data open possibilities for the use of different tetracycline targets for structural studies in solution.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(00)01728-2</identifier><identifier>PMID: 10908732</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Binding site ; Molecular Sequence Data ; Photo-activated affinity modification ; Photoaffinity Labels ; Protein sequencing ; Repressor Proteins - chemistry ; Tetracycline ; Tetracycline - chemistry ; Transcription repressor protein TetR(D) ; X-Ray Diffraction</subject><ispartof>FEBS letters, 2000-07, Vol.477 (3), p.263-267</ispartof><rights>2000 Federation of European Biochemical Societies</rights><rights>FEBS Letters 477 (2000) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4262-267c8665bae652911ef45d2d0c5f79adfe9f01a58642f960e62ee6d91c355ad03</citedby><cites>FETCH-LOGICAL-c4262-267c8665bae652911ef45d2d0c5f79adfe9f01a58642f960e62ee6d91c355ad03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2800%2901728-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579300017282$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27903,27904,45553,45554,46387,46811,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10908732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beliakova, Maria M.</creatorcontrib><creatorcontrib>Anokhina, Maria M.</creatorcontrib><creatorcontrib>Spiridonova, Vera A.</creatorcontrib><creatorcontrib>Dobrov, Eugeny N.</creatorcontrib><creatorcontrib>Egorov, Tsezi A.</creatorcontrib><creatorcontrib>Wittmann-Liebold, Brigitte</creatorcontrib><creatorcontrib>Orth, Peter</creatorcontrib><creatorcontrib>Saenger, Wolfram</creatorcontrib><creatorcontrib>Kopylov, Alexei M.</creatorcontrib><title>A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Results of a first successful application of a direct photo-induced affinity modification of Tet repressor (TetR(D)) protein with tetracycline within a complex of known three-dimensional structure are described. The conditions of the modification have provided suitable yields of the modified complex and allowed characterization of the modified segments of the protein. The potential of tetracycline as a fine modifying reagent was established. In the complex of TetR(D) protein with tetracycline, the antibiotic modifies at least two segments, Ile59–Glu73 and Ala173–Glu183, which form a binding tunnel for the drug according to the X-ray analysis. These data open possibilities for the use of different tetracycline targets for structural studies in solution.</description><subject>Amino Acid Sequence</subject><subject>Binding site</subject><subject>Molecular Sequence Data</subject><subject>Photo-activated affinity modification</subject><subject>Photoaffinity Labels</subject><subject>Protein sequencing</subject><subject>Repressor Proteins - chemistry</subject><subject>Tetracycline</subject><subject>Tetracycline - chemistry</subject><subject>Transcription repressor protein TetR(D)</subject><subject>X-Ray Diffraction</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1TAQhS0EopfCTwB5hdpFqO3ETrJCpQ9aqRISlLXl2mN1UG4cbN-W--_r3FSoXcHKjznnzOgbQt5z9okzro5-MMabSrZ9fcDYIeOt6Crxgqx419ZV3ajuJVn9leyRNyn9YuXd8f412eOsZ0UnVuTPMXUYwWY63YYcKmMz3pkMjhrvccS8pevg0KM1GcNIg6cZcjR2awccgZbrmGzEaVeNMEVIKUQ6xZABR3oN-fvB6SG9x3z7zPmWvPJmSPDu8dwnP8_Prk8uqqtvXy9Pjq8q2wglKqFa2yklbwwoKXrOwTfSCces9G1vnIfeM25kpxrhe8VACQDlem5rKY1j9T75uOSWiX5vIGW9xmRhGMwIYZN0y4VUdc2LUC5CG0NKEbyeIq5N3GrO9Ixc75DrmadmTO-Qa1F8Hx4bbG7W4J64FsZFcLEI7nGA7f-l6vOzL2JXmQvz4sr3HPV5iYJC7A4h6mQRRgvLDrUL-I9pHwDMgKbN</recordid><startdate>20000721</startdate><enddate>20000721</enddate><creator>Beliakova, Maria M.</creator><creator>Anokhina, Maria M.</creator><creator>Spiridonova, Vera A.</creator><creator>Dobrov, Eugeny N.</creator><creator>Egorov, Tsezi A.</creator><creator>Wittmann-Liebold, Brigitte</creator><creator>Orth, Peter</creator><creator>Saenger, Wolfram</creator><creator>Kopylov, Alexei M.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000721</creationdate><title>A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline</title><author>Beliakova, Maria M. ; Anokhina, Maria M. ; Spiridonova, Vera A. ; Dobrov, Eugeny N. ; Egorov, Tsezi A. ; Wittmann-Liebold, Brigitte ; Orth, Peter ; Saenger, Wolfram ; Kopylov, Alexei M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4262-267c8665bae652911ef45d2d0c5f79adfe9f01a58642f960e62ee6d91c355ad03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino Acid Sequence</topic><topic>Binding site</topic><topic>Molecular Sequence Data</topic><topic>Photo-activated affinity modification</topic><topic>Photoaffinity Labels</topic><topic>Protein sequencing</topic><topic>Repressor Proteins - chemistry</topic><topic>Tetracycline</topic><topic>Tetracycline - chemistry</topic><topic>Transcription repressor protein TetR(D)</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Beliakova, Maria M.</creatorcontrib><creatorcontrib>Anokhina, Maria M.</creatorcontrib><creatorcontrib>Spiridonova, Vera A.</creatorcontrib><creatorcontrib>Dobrov, Eugeny N.</creatorcontrib><creatorcontrib>Egorov, Tsezi A.</creatorcontrib><creatorcontrib>Wittmann-Liebold, Brigitte</creatorcontrib><creatorcontrib>Orth, Peter</creatorcontrib><creatorcontrib>Saenger, Wolfram</creatorcontrib><creatorcontrib>Kopylov, Alexei M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beliakova, Maria M.</au><au>Anokhina, Maria M.</au><au>Spiridonova, Vera A.</au><au>Dobrov, Eugeny N.</au><au>Egorov, Tsezi A.</au><au>Wittmann-Liebold, Brigitte</au><au>Orth, Peter</au><au>Saenger, Wolfram</au><au>Kopylov, Alexei M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2000-07-21</date><risdate>2000</risdate><volume>477</volume><issue>3</issue><spage>263</spage><epage>267</epage><pages>263-267</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Results of a first successful application of a direct photo-induced affinity modification of Tet repressor (TetR(D)) protein with tetracycline within a complex of known three-dimensional structure are described. The conditions of the modification have provided suitable yields of the modified complex and allowed characterization of the modified segments of the protein. The potential of tetracycline as a fine modifying reagent was established. In the complex of TetR(D) protein with tetracycline, the antibiotic modifies at least two segments, Ile59–Glu73 and Ala173–Glu183, which form a binding tunnel for the drug according to the X-ray analysis. These data open possibilities for the use of different tetracycline targets for structural studies in solution.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10908732</pmid><doi>10.1016/S0014-5793(00)01728-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2000-07, Vol.477 (3), p.263-267 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71256331 |
| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Free Content; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Binding site Molecular Sequence Data Photo-activated affinity modification Photoaffinity Labels Protein sequencing Repressor Proteins - chemistry Tetracycline Tetracycline - chemistry Transcription repressor protein TetR(D) X-Ray Diffraction |
| title | A direct photo-activated affinity modification of tetracycline transcription repressor protein TetR(D) with tetracycline |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T20%3A47%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20direct%20photo-activated%20affinity%20modification%20of%20tetracycline%20transcription%20repressor%20protein%20TetR(D)%20with%20tetracycline&rft.jtitle=FEBS%20letters&rft.au=Beliakova,%20Maria%20M.&rft.date=2000-07-21&rft.volume=477&rft.issue=3&rft.spage=263&rft.epage=267&rft.pages=263-267&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/S0014-5793(00)01728-2&rft_dat=%3Cproquest_cross%3E71256331%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71256331&rft_id=info:pmid/10908732&rft_els_id=S0014579300017282&rfr_iscdi=true |