Pax4 Represses Pancreatic Glucagon Gene Expression
The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon g...
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| Veröffentlicht in: | Molecular cell biology research communications 2000-04, Vol.3 (4), p.249-254 |
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| container_end_page | 254 |
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| container_title | Molecular cell biology research communications |
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| creator | Petersen, Helle V. Jørgensen, Mette C. Andersen, Frank G. Jensen, Jan F-Nielsen, Tove Jørgensen, Ragna Madsen, Ole D. Serup, Palle |
| description | The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6. |
| doi_str_mv | 10.1006/mcbr.2000.0220 |
| format | Article |
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In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. 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In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.</description><subject>Animals</subject><subject>Cell Line</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Eye Proteins</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation</subject><subject>glucagon</subject><subject>Glucagon - genetics</subject><subject>Glucagon - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>In Situ Hybridization</subject><subject>insulin</subject><subject>Insulin - genetics</subject><subject>Islets of Langerhans - metabolism</subject><subject>Mice</subject><subject>Paired Box Transcription Factors</subject><subject>Pax4</subject><subject>Pax6</subject><subject>PAX6 Transcription Factor</subject><subject>Pdx1</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Rats</subject><subject>repression</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>1522-4724</issn><issn>1522-4732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LxDAQhoMorq5ePUpP3lqTNN00R1nWVVhwET2HJJ1IpB9r0sr6703tIl48ZQjPvDPzIHRFcEYwXtw2RvuMYowzTCk-QmekoDRlPKfHvzVlM3QewnukFoTwUzQjuBSEYXyG6FbtWfIMOw8hQEi2qjUeVO9Msq4Ho966NllDC8lq_4O4rr1AJ1bVAS4P7xy93q9elg_p5mn9uLzbpIYWRZ8ybZS1tuTWAq6UMphrwYEpoY1mcdsFKF2ZvCwFy2luBcNVwXWlbR7_uM7n6GbK3fnuY4DQy8YFA3WtWuiGIDmJcwQTEcwm0PguBA9W7rxrlP-SBMvRkhwtydGSHC3FhutD8qAbqP7gk5YIlBMA8b5PB14G46A1UDkPppdV5_7L_gZF3HYK</recordid><startdate>200004</startdate><enddate>200004</enddate><creator>Petersen, Helle V.</creator><creator>Jørgensen, Mette C.</creator><creator>Andersen, Frank G.</creator><creator>Jensen, Jan</creator><creator>F-Nielsen, Tove</creator><creator>Jørgensen, Ragna</creator><creator>Madsen, Ole D.</creator><creator>Serup, Palle</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200004</creationdate><title>Pax4 Represses Pancreatic Glucagon Gene Expression</title><author>Petersen, Helle V. ; Jørgensen, Mette C. ; Andersen, Frank G. ; Jensen, Jan ; F-Nielsen, Tove ; Jørgensen, Ragna ; Madsen, Ole D. ; Serup, Palle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c255t-4bcafff87ffe0daac07b97e4a9bcb40226eabdc38894323f940d57bdbf33887b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Eye Proteins</topic><topic>Fluorescent Antibody Technique</topic><topic>Gene Expression Regulation</topic><topic>glucagon</topic><topic>Glucagon - genetics</topic><topic>Glucagon - metabolism</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>In Situ Hybridization</topic><topic>insulin</topic><topic>Insulin - genetics</topic><topic>Islets of Langerhans - metabolism</topic><topic>Mice</topic><topic>Paired Box Transcription Factors</topic><topic>Pax4</topic><topic>Pax6</topic><topic>PAX6 Transcription Factor</topic><topic>Pdx1</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Rats</topic><topic>repression</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petersen, Helle V.</creatorcontrib><creatorcontrib>Jørgensen, Mette C.</creatorcontrib><creatorcontrib>Andersen, Frank G.</creatorcontrib><creatorcontrib>Jensen, Jan</creatorcontrib><creatorcontrib>F-Nielsen, Tove</creatorcontrib><creatorcontrib>Jørgensen, Ragna</creatorcontrib><creatorcontrib>Madsen, Ole D.</creatorcontrib><creatorcontrib>Serup, Palle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cell biology research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petersen, Helle V.</au><au>Jørgensen, Mette C.</au><au>Andersen, Frank G.</au><au>Jensen, Jan</au><au>F-Nielsen, Tove</au><au>Jørgensen, Ragna</au><au>Madsen, Ole D.</au><au>Serup, Palle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pax4 Represses Pancreatic Glucagon Gene Expression</atitle><jtitle>Molecular cell biology research communications</jtitle><addtitle>Mol Cell Biol Res Commun</addtitle><date>2000-04</date><risdate>2000</risdate><volume>3</volume><issue>4</issue><spage>249</spage><epage>254</epage><pages>249-254</pages><issn>1522-4724</issn><eissn>1522-4732</eissn><abstract>The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10891400</pmid><doi>10.1006/mcbr.2000.0220</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Cell Line DNA-Binding Proteins - physiology Eye Proteins Fluorescent Antibody Technique Gene Expression Regulation glucagon Glucagon - genetics Glucagon - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism In Situ Hybridization insulin Insulin - genetics Islets of Langerhans - metabolism Mice Paired Box Transcription Factors Pax4 Pax6 PAX6 Transcription Factor Pdx1 Promoter Regions, Genetic - genetics Rats repression Repressor Proteins - genetics Repressor Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transfection Tumor Cells, Cultured |
| title | Pax4 Represses Pancreatic Glucagon Gene Expression |
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