Chromatography of isoforms of recombinant apoaequorin and method for the preparation of aequorin

Gradient elution chromatography of recombinant apoaequorin carried out in the presence of Ca 2+ revealed two isoforms of apoaequorin, reduced and oxidized, whereas in the presence of EDTA 3 isoforms were observed. In a regeneration mixture of apoaequorin, coelenterazine, EDTA, and 2-mercaptoethanol,...

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Veröffentlicht in:	Protein expression and purification 2003-10, Vol.31 (2), p.181-187
Hauptverfasser:	Masuda, Hiromi, Takenaka, Yasuhiro, Shikamoto, Yasuo, Kagawa, Masayuki, Mizuno, Hiroshi, Tsuji, Frederick I
Format:	Artikel
Sprache:	eng
Schlagworte:	Aequorin - genetics Aequorin - isolation & purification Aequorin - metabolism Animals Apoproteins - genetics Apoproteins - isolation & purification Apoproteins - metabolism Bioluminescence Blue fluorescent protein Calcium - pharmacology Calcium determination Chromatography Coelenterazine Electrophoresis, Polyacrylamide Gel Photoprotein Protein Engineering Protein Isoforms - genetics Protein Isoforms - isolation & purification Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Time Factors
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