Decreased CD10-positive mature granulocytes in bone marrow from patients with myelodysplastic syndrome
We retrospectively examined the maturation of the granulocytic cell lineage in bone marrow specimens from patients with myelodysplastic syndrome (MDS) by flow cytometry using both light scatter and surface marker characteristics, including CD10 and myeloid lineage-associated antigens. The 7 MDS case...
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| Veröffentlicht in: | Archives of pathology & laboratory medicine (1976) 2000-08, Vol.124 (8), p.1152-1156 |
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| container_title | Archives of pathology & laboratory medicine (1976) |
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| creator | Chang, C C Cleveland, R P |
| description | We retrospectively examined the maturation of the granulocytic cell lineage in bone marrow specimens from patients with myelodysplastic syndrome (MDS) by flow cytometry using both light scatter and surface marker characteristics, including CD10 and myeloid lineage-associated antigens.
The 7 MDS cases we studied included 2 patients with refractory anemia (RA), 3 with RA with ringed sideroblasts, 1 with RA with excess of blasts, and 1 unclassified case. Another 7 patients matched for age and sex who received bone marrow aspirates for lymphoma staging (all negative for lymphoma involvement or any other hematologic abnormalities) were selected as the control group.
The percentage of CD10(+) mature granulocytes was significantly lower in patients with MDS than in control patients. Additionally, all patients with MDS had less than 50% CD10(+) cells in the granulocytic lineage. In contrast, only 1 of the 7 control patients had less than 50% CD10(+) cells (P |
| doi_str_mv | 10.5858/2000-124-1152-DCPMGI |
| format | Article |
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The 7 MDS cases we studied included 2 patients with refractory anemia (RA), 3 with RA with ringed sideroblasts, 1 with RA with excess of blasts, and 1 unclassified case. Another 7 patients matched for age and sex who received bone marrow aspirates for lymphoma staging (all negative for lymphoma involvement or any other hematologic abnormalities) were selected as the control group.
The percentage of CD10(+) mature granulocytes was significantly lower in patients with MDS than in control patients. Additionally, all patients with MDS had less than 50% CD10(+) cells in the granulocytic lineage. In contrast, only 1 of the 7 control patients had less than 50% CD10(+) cells (P <.01).
These results suggest that flow cytometry might be a useful adjunct in the assessment of patients with suspected MDS. Further studies to correlate CD10(+) mature granulocytes from MDS cases with other benign hematologic disorders are indicated to confirm our evaluation.</description><identifier>ISSN: 0003-9985</identifier><identifier>ISSN: 1543-2165</identifier><identifier>EISSN: 1543-2165</identifier><identifier>DOI: 10.5858/2000-124-1152-DCPMGI</identifier><identifier>PMID: 10923075</identifier><language>eng</language><publisher>United States: College of American Pathologists</publisher><subject>Aged ; Aged, 80 and over ; Antigens, Differentiation - metabolism ; Bone Marrow - immunology ; Bone Marrow - pathology ; Bone Marrow Examination ; Cell Lineage ; Diagnosis ; Disease Progression ; Female ; Flow Cytometry ; Granulocytes - cytology ; Granulocytes - metabolism ; HLA-DR1 Antigen - metabolism ; Humans ; Immunophenotyping ; Male ; Middle Aged ; Myelodysplastic syndromes ; Myelodysplastic Syndromes - immunology ; Myelodysplastic Syndromes - metabolism ; Myelodysplastic Syndromes - pathology ; Neprilysin - metabolism ; Prognosis ; Retrospective Studies</subject><ispartof>Archives of pathology & laboratory medicine (1976), 2000-08, Vol.124 (8), p.1152-1156</ispartof><rights>COPYRIGHT 2000 College of American Pathologists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-ba2f66f4370757c81ba9e188bf1739949000cdb7bd6ff354c32311366ce02ed23</citedby><cites>FETCH-LOGICAL-c408t-ba2f66f4370757c81ba9e188bf1739949000cdb7bd6ff354c32311366ce02ed23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10923075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, C C</creatorcontrib><creatorcontrib>Cleveland, R P</creatorcontrib><title>Decreased CD10-positive mature granulocytes in bone marrow from patients with myelodysplastic syndrome</title><title>Archives of pathology & laboratory medicine (1976)</title><addtitle>Arch Pathol Lab Med</addtitle><description>We retrospectively examined the maturation of the granulocytic cell lineage in bone marrow specimens from patients with myelodysplastic syndrome (MDS) by flow cytometry using both light scatter and surface marker characteristics, including CD10 and myeloid lineage-associated antigens.
The 7 MDS cases we studied included 2 patients with refractory anemia (RA), 3 with RA with ringed sideroblasts, 1 with RA with excess of blasts, and 1 unclassified case. Another 7 patients matched for age and sex who received bone marrow aspirates for lymphoma staging (all negative for lymphoma involvement or any other hematologic abnormalities) were selected as the control group.
The percentage of CD10(+) mature granulocytes was significantly lower in patients with MDS than in control patients. Additionally, all patients with MDS had less than 50% CD10(+) cells in the granulocytic lineage. In contrast, only 1 of the 7 control patients had less than 50% CD10(+) cells (P <.01).
These results suggest that flow cytometry might be a useful adjunct in the assessment of patients with suspected MDS. Further studies to correlate CD10(+) mature granulocytes from MDS cases with other benign hematologic disorders are indicated to confirm our evaluation.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Bone Marrow - immunology</subject><subject>Bone Marrow - pathology</subject><subject>Bone Marrow Examination</subject><subject>Cell Lineage</subject><subject>Diagnosis</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Granulocytes - cytology</subject><subject>Granulocytes - metabolism</subject><subject>HLA-DR1 Antigen - metabolism</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Myelodysplastic syndromes</subject><subject>Myelodysplastic Syndromes - immunology</subject><subject>Myelodysplastic Syndromes - metabolism</subject><subject>Myelodysplastic Syndromes - pathology</subject><subject>Neprilysin - metabolism</subject><subject>Prognosis</subject><subject>Retrospective Studies</subject><issn>0003-9985</issn><issn>1543-2165</issn><issn>1543-2165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU-P1CAYh4nRuOPqNzCGi55EC5TSHjczum6yRg96JpS-zGBoqUB3028vk-5hTTYc-Pe8b_jxIPSWVp9EK9rPrKoqQllNKBWMHPY_v1_fPEM7KmpOGG3Ec7QrBCdd14oL9CqlP2XbMUZfogtaFrySYofsAUwEnWDA-wOtyBySy-4O8KjzEgEfo54WH8yaIWE34T5M57sYwz22MYx41tnBlBO-d_mExxV8GNY0e52yMzit01AoeI1eWO0TvHmYL9Hvr19-7b-R2x_XN_urW2Lqqs2k18w2ja25LI-TpqW97oC2bW-p5F1XdyWCGXrZD421XNSGM04pbxoDFYOB8Uv0Yes7x_B3gZTV6JIB7_UEYUlKUiaY5HUBP27gUXtQbrIhR22OMEHUvmS0rhxfNUJKVktacPIEXsYAozNP8e8f8SfQPp9S8Et2YUr_cfXGmRhSimDVHF353lXRSp01q7NmVTSrs2a1aS5l7x5iLv0Iw6OizSv_BzAPo48</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Chang, C C</creator><creator>Cleveland, R P</creator><general>College of American Pathologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Decreased CD10-positive mature granulocytes in bone marrow from patients with myelodysplastic syndrome</title><author>Chang, C C ; Cleveland, R P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-ba2f66f4370757c81ba9e188bf1739949000cdb7bd6ff354c32311366ce02ed23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, Differentiation - metabolism</topic><topic>Bone Marrow - immunology</topic><topic>Bone Marrow - pathology</topic><topic>Bone Marrow Examination</topic><topic>Cell Lineage</topic><topic>Diagnosis</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Granulocytes - cytology</topic><topic>Granulocytes - metabolism</topic><topic>HLA-DR1 Antigen - metabolism</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Myelodysplastic syndromes</topic><topic>Myelodysplastic Syndromes - immunology</topic><topic>Myelodysplastic Syndromes - metabolism</topic><topic>Myelodysplastic Syndromes - pathology</topic><topic>Neprilysin - metabolism</topic><topic>Prognosis</topic><topic>Retrospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, C C</creatorcontrib><creatorcontrib>Cleveland, R P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of pathology & laboratory medicine (1976)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, C C</au><au>Cleveland, R P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased CD10-positive mature granulocytes in bone marrow from patients with myelodysplastic syndrome</atitle><jtitle>Archives of pathology & laboratory medicine (1976)</jtitle><addtitle>Arch Pathol Lab Med</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>124</volume><issue>8</issue><spage>1152</spage><epage>1156</epage><pages>1152-1156</pages><issn>0003-9985</issn><issn>1543-2165</issn><eissn>1543-2165</eissn><abstract>We retrospectively examined the maturation of the granulocytic cell lineage in bone marrow specimens from patients with myelodysplastic syndrome (MDS) by flow cytometry using both light scatter and surface marker characteristics, including CD10 and myeloid lineage-associated antigens.
The 7 MDS cases we studied included 2 patients with refractory anemia (RA), 3 with RA with ringed sideroblasts, 1 with RA with excess of blasts, and 1 unclassified case. Another 7 patients matched for age and sex who received bone marrow aspirates for lymphoma staging (all negative for lymphoma involvement or any other hematologic abnormalities) were selected as the control group.
The percentage of CD10(+) mature granulocytes was significantly lower in patients with MDS than in control patients. Additionally, all patients with MDS had less than 50% CD10(+) cells in the granulocytic lineage. In contrast, only 1 of the 7 control patients had less than 50% CD10(+) cells (P <.01).
These results suggest that flow cytometry might be a useful adjunct in the assessment of patients with suspected MDS. Further studies to correlate CD10(+) mature granulocytes from MDS cases with other benign hematologic disorders are indicated to confirm our evaluation.</abstract><cop>United States</cop><pub>College of American Pathologists</pub><pmid>10923075</pmid><doi>10.5858/2000-124-1152-DCPMGI</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Allen Press Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Aged Aged, 80 and over Antigens, Differentiation - metabolism Bone Marrow - immunology Bone Marrow - pathology Bone Marrow Examination Cell Lineage Diagnosis Disease Progression Female Flow Cytometry Granulocytes - cytology Granulocytes - metabolism HLA-DR1 Antigen - metabolism Humans Immunophenotyping Male Middle Aged Myelodysplastic syndromes Myelodysplastic Syndromes - immunology Myelodysplastic Syndromes - metabolism Myelodysplastic Syndromes - pathology Neprilysin - metabolism Prognosis Retrospective Studies |
| title | Decreased CD10-positive mature granulocytes in bone marrow from patients with myelodysplastic syndrome |
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