Structure of the Bordetella pertussis 1414 endotoxin

The endotoxin (lipopolysaccharide) of Bordetella pertussis, the agent of whooping cough, consists of a lipid A linked to a highly branched dodecasaccharide containing several acid and amino sugars. The elucidation of the polysaccharide structure was accomplished by first analyzing the structures of...

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Veröffentlicht in:	FEBS letters 2000-07, Vol.477 (1), p.8-14
Hauptverfasser:	Caroff, Martine, Brisson, Jean-Robert, Martin, Adèle, Karibian, Doris
Format:	Artikel
Sprache:	eng
Schlagworte:	Amination Amines - metabolism Bordetella pertussis Bordetella pertussis - chemistry Carbohydrate Conformation Carbohydrate Sequence COSY, correlated spectroscopy d-ManH, dehydromannitol Endotoxin Fuc2NAc4NMe, 2-acetamido-4-N-methyl-2,4,6-deoxy-galactose FucNAcMe, 2-acetamido-2-N-methyl-2,6-deoxy-galactose Glc, glucose GlcA, glucuronic acid GlcNAc, N-acetyl glucosamine Hep, heptose HMBC, heteronuclear multiple bond connectivity HSQC, heteronuclear single quantum coherence Hydrolysis Kdo, 3-deoxy-D-manno-octulopyranosonic acid Lipid A - analysis Lipid A - metabolism Lipopolysaccharides - chemistry Lipopolysaccharides - metabolism LPS, lipopolysaccharide Magnetic Resonance Spectroscopy MALDI, matrix-assisted laser desorption/ionization mass spectrometry Man2NAc3NAcA, 2-acetamido-3-acetamido-2,3-dideoxy-mannuronic acid Matrix-assisted laser desorption/ionization mass spectrometry Molecular Sequence Data NOESY, nuclear Overhauser enhancement spectroscopy Nuclear magnetic resonance Oligosaccharides - analysis Oligosaccharides - chemistry Oligosaccharides - metabolism PPEA, pyrophosphorylethanolamine Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Structure TOCSY, total correlated spectroscopy
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