Role of alcohol dehydrogenase E (ADHE) in the energy metabolism of Giardia lamblia

Two hammerhead ribozymes flanked by Giardia lamblia alcohol dehydrogenase E (ADHE) antisense RNA fragments, ARzS and ARzL, were designed, synthesized and found capable of cleaving an ADHE mRNA fragment at the anticipated position in vitro. The ribozymes were then electroporated into Giardia trophozo...

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Veröffentlicht in:	Molecular and biochemical parasitology 2000-06, Vol.109 (1), p.25-36
Hauptverfasser:	Dan, Meixia, Wang, Ching C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aerobiosis Alcohol Dehydrogenase - genetics Alcohol Dehydrogenase - metabolism Alcohol Dehydrogenase - physiology alcohol dehydrogenase E Anaerobiosis Animals Antisense RNA Electroporation Energy Metabolism Gene expression Genes, Protozoan Genetic Vectors Giardia lamblia Giardia lamblia - genetics Giardia lamblia - metabolism Giardia lamblia - virology Giardiavirus Giardiavirus - genetics Hammerhead ribozyme Molecular Sequence Data NAD - metabolism RNA, Antisense - genetics RNA, Catalytic - genetics RNA, Catalytic - metabolism RNA, Messenger - metabolism Transfection
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